Tiania quercicola Ning Jiang, 2025

Jiang, Ning, Xue, Han & Li, Yong, 2025, Novel genus and species of Diaporthostomataceae (Diaporthales) in China, IMA Fungus 16, pp. e 145422-e 145422 : e145422-

Tiania quercicola Ning Jiang sp. nov.

Fig. 6

Etymology.

Named after the host genus Quercus and “ - cola ” = “ inhabiting ”.

Diagnosis.

Distinct from T. chinensis and T. lithocarpicola by conidia that are constricted at the middle.

Typus.

CHINA • Hainan Province, Changjiang Li Autonomous County, Bawangling National Forest Park, on diseased leaves of Quercus macrocalyx , 30 March 2019, Yong Li (holotype CAF 800035 ; ex-holotype culture CFCC 54435 ) .

Description.

Conidiomata in culture sporodochial, aggregated, erumpent, pulvinate, light orange, 150–700 μm diam., exuding light orange conidial masses. Conidiophores hyaline, smooth, cylindrical, branched, usually reduced to conidiogenous cells. Conidiogenous cells hyaline, smooth, cylindrical to ampulliform, attenuate towards the apex, phialidic, 10.5–21.5 × 1–2.5 μm. Conidia aseptate, hyaline, smooth, multi-guttulate, cylindrical, constricted at the middle, straight or slightly curved, base truncate, 5.5–7 (– 8) × 2–2.5 μm (n = 50), L / W = 1.6–2.7.

Culture characteristics.

Colonies on PDA flat, spreading, with abundant flocculent aerial mycelium and even margin, initially white, becoming umber after 1 wk, reaching 90 mm diam after 2 wk at 25 ° C. Colonies on MEA flat, spreading, with abundant flocculent aerial mycelium and undulating margin, white to smoke grey, reaching 90 mm diam after 2 wk at 25 ° C. Colonies on SNA flat, spreading, with sparse flocculent aerial mycelium and feathery margin, white, reaching 90 mm diam after 3 wk at 25 ° C.

Additional material examined.

CHINA • Hainan Province, Changjiang Li Autonomous County, Bawangling National Forest Park, from leaf spots of Quercus macrocalyx , 30 March 2019, Yong Li (living culture CFCC 55885 ) .

Distribution.

China, Hainan Province.

Ecology.

Associated with leaf spot disease with Quercus macrocalyx .

Notes.

Tiania quercicola , isolated from Quercus macrocalyx , is phylogenetically closely related to T. chinensis from Quercus semecarpifolia and Q. spinosa , and T. lithocarpicola from Lithocarpus elaeagnifolius (Fig. 1 ). However, T. quercicola can be distinguished from these two species by its conidia, which are constricted at the middle.

Figures

Figure 1. Phylogram of Diaporthales resulting from a maximum likelihood analysis based on the ITS, LSU, rpb 2 and tef 1 gene sequence. Numbers above the branches indicate ML bootstrap values (left, ML BS ≥ 50 %) and Bayesian posterior probabilities (right, BPP ≥ 0.90). The tree is rooted with Nakataea oryzae (CBS 243.76) and Pyricularia grisea (Ina 168). Isolates from the present study are marked in blue.

Figure 6. Tiania quercicola sp. nov. from Quercus macrocalyx (CAF 800035). A Colonies on PDA, MEA and SNA after 10 d at 25 ° C; B conidiomata formed on PDA; C conidiogenous cells; D – G conidia. Scale bars: 300 μm (B); 10 μm (C – G).