Rhizoctonia bulbillifaciens (Diederich & Lawrey) O’Donnell, R. P., Linde, C. C. & May, T. W., 2025

O’Donnell, R. P., Linde, C. C. & May, T. W., 2025, Rise up, Rhizoctonia: moving to one fungus, one name in the Ceratobasidiaceae (Agaricomycetes, Cantharellales), Persoonia 54 (1), pp. 285-325 : 302

Rhizoctonia bulbillifaciens (Diederich & Lawrey) R.P. O’Donnell, C.C. Linde & T.W. May, comb. nov. MB 852038 .

Basionym: Ceratobasidium bulbillifaciens Diederich & Lawrey, Lichenologist 46 (3): 345. 2014.

Type citation: ‘Type: Germany, Hessen, Giessen, Parkplatz bei Sporthalle im süden von Heuchelheim, alt. 155 m, on Acer platanoides , on unidentified, dying lichenized crust, also on young thalli of Physcia tenella , 6 September 2010, R. Cezanne & M. Eichler 8193 ( BR – holotype; hb. Diederich– isotype). Ex-type culture: CBS 129339. ‘[‘Type: Germany, Hessen, Giessen. Parking lot at the sport hall in the south of Heuchelheim...’].

ITS barcode: OR471309 ( T); KC336072 ( R).

UNITE 1.5 % SH: SH0961369.10FU.

Alternative markers: LSU = KC336071 (R); RPB2 OR473843 (R); TEF 1 = OR490360 (R).

Ex-type culture: CBS 129339. Notes: In the protologue for Cb. bulbillifaciens, CBS 129339 is cited as the ex-type culture for the collection “Eichler-Cezanne 8193”; however, there was no ITS sequence from this culture generated by the authors in their published phylogeny as “another sequence (Eichler-Cezanne 8193) proved to be too short to be reliably placed phylogenetically” (p. 338). Instead, the authors incorporated a single ITS sequence from CBS 132236 which represents the collection “Eichler-Cezanne 8067”, listed as an additional specimen examined. Diederich et al. (2014) inferred a separate LSU tree which did incorporate sequences from both CBS 129339 and CBS 132236, which were clustered together. Sequences of the ITS region from the ex-type culture (CBS 129339) were later generated by Swenie et al. (2024). When BLASTed, the ITS sequence from CBS 132236 published by Diederich et al. (2014) was found to share> 99 % similarity to ITS sequences generated from the ex-type culture by Swenie et al. (2024). Sequences from both accessions are clustered within the UNITE 1.5 % SH SH0961369.10FU. On this basis, CBS 129339 and CBS 132236 should indeed be considered conspecific. There are sequences of the RPB2 and TEF 1 regions for Cb. bulbillifaciens available on GenBank; however, they have been sequenced from CBS 132236. As sequences for the ITS, LSU, RPB2, and TEF 1 regions were available for CBS 132236 and not CBS 129339, sequences representing CBS 132236 were selected for phylogenetic analyses in this study to ensure all regions incorporated were from the same collection. These sequences are cited here as representative sequences in addition to the type ITS barcode.