Phyllozyma diaoluoensis Z. W. Xi & F. L. Hui, 2025

Xi, Zhi-Wen, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Phylogeny of Phyllozyma (Spiculogloeaceae, Spiculogloeales) with description of four new species from China, MycoKeys 123, pp. 235-251 : 235-251

Phyllozyma diaoluoensis Z. W. Xi & F. L. Hui sp. nov.

Fig. 5

Etymology.

The specific epithet “ diaoluoensis ” refers to the geographic origin of the type strain, Diaoluo Mountain, Qiongzhong Li and Miao Autonomous County, Hainan Province.

Typus.

China • Hainan Prov.: Qiongzhong Li and Miao Autonomous County, Diaoluo Mountain , in the phylloplane of Zanthoxylum avicennae , 15 Jul 2023, X. M. Han, NYNU 2377 ( holotype CICC 33620 T preserved as a metabolically inactive state, culture ex-type PYCC 9983 ) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is pale-yellow, tough, and somewhat wrinkled, with an entire margin. After 3 days in YM broth at 20 ° C, cells are cylindrical, 2.0–2.8 × 4.3–6.8 μm and single, budding is polar. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA, or V 8 agar. Ballistoconidia are falcate or cylindrical, 2.5–4.9 × 8.7–12.6 μm. Glucose fermentation is absent. Glucose, inulin, sucrose, raffinose (delayed), galactose, trehalose (delayed), D-arabinose (weak), glycerol, ribitol (weak), D-mannitol (weak), D-glucitol, succinate (weak), citrate (weak), and D-glucuronate (weak) are assimilated as sole carbon sources. Melibiose, lactose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, 5 - keto-D-gluconate, D-ribose, methanol, ethanol, erythritol, galactitol, myo-inositol, DL-lactate, D-gluconate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate, and D-glucono- 1, 5 - lactone are not assimilated. Nitrate (delayed) and nitrite (delayed) are assimilated as sole nitrogen sources. Ethylamine, L-lysine, and cadaverine are not assimilated. Maximum growth temperature is 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Hainan Prov.: Qiongzhong Li and Miao Autonomous County, Diaoluo Mountain , in the phylloplane of Gordonia hainanensis , 15 Jul 2023, X. M. Han, NYNU 23732 ; in the phylloplane of Altingia obovata , 15 Jul 2023, X. M. Han, NYNU 23718 .

GenBank accession numbers.

Holotype CICC 33620 T ( ITS: OR 526726, D 1 / D 2: OR 511464, RPB 1: PX 353016, TEF 1: PV 654544); additional strains NYNU 23732 ( ITS: OR 961462, D 1 / D 2: OR 958779, RPB 1: PX 353017, TEF 1: PV 654545) and NYNU 23718 ( ITS: OR 958777, D 1 / D 2: OR 958778, RPB 1: PX 353018, TEF 1: PV 654546).

Note.

Physiologically, P. diaoluoensis sp. nov. differs from its closely related species P. jiayinensis in its ability to assimilate inulin, sucrose, raffinose, galactose, trehalose, D-arabinose, glycerol, ribitol, succinate, and citrate and its inability to assimilate DL-Lactate. Furthermore, P. diaoluoensis sp. nov. can grow at 30 ° C, while P. jiayinensis cannot (Table 2 ).

Figures

Figure 5. Morphological characteristics of P. diaoluoensis sp. nov. NYNU 2377 T. A. The streak culture grown on YM agar after 7 d at 20 ° C; B. Budding cells grown in YM broth for 3 d at 20 ° C; C. Ballistoconidia produced on CMA after 7 d at 20 ° C. Scale bars: 10 μm.