Nemania primolutea Y. M. Ju, H. M. Hsieh and J. D. Rogers

Absalan, Sahar, Armand, Alireza, Jayawardena, Ruvishika S., Suwannarach, Nakarin, Monkai, Jutamart, Jungkhun, Nootjarin, Lumyong, Saisamorn & Hyde, Kevin D., 2025, Endophytic xylariaceous fungi from rice in northern Thailand: discovery of novel species and new host records, MycoKeys 120, pp. 1-34 : 1-34

Nemania primolutea Y. M. Ju, H. M. Hsieh and J. D. Rogers , Mycologia 97: 567 (2005)

Fig. 8

Description.

Endophytic from healthy panicle of Oryza sativa . Sexual morph: Not observed. Asexual morph: Hyphomycetous. Conidiophores 1.5–4 µm wide, unbranched or sometimes dichotomously branched, smooth-walled, pale brown to hyaline. Conidiogenous cells 1.5–2.5 µm wide, cylindrical, geniculate, proliferating unclearly, smooth-walled, hyaline. Conidia 2.4–4.5 × 5.5–8.4 µm (x ̄ = 3.5 × 6.4 µm, n = 30), ellipsoid to obovoid, smooth, hyaline.

Culture characteristics.

Colonies on PDA reaching 69–73 mm in diameter after 7 days at 28 ° C, white, circular, velvety, slightly raised, with crenate margins; reverse yellowish white.

Material examined.

Thailand • Chiang Rai Province, Thoeng District, San Sai Ngam Sub-District , from healthy tissue part of rice panicle, 9 November 2021, Nootjarin Jungkhun (NS 39-1 a = MFLU 25-0026 ) (living culture MFLUCC 24-0507 ) .

GenBank numbers.

MFLUCC 24-0507 : ITS = PV 241498, LSU = PV 235235, rpb 2 = PV 275681, tub 2 = PV 275685.

Notes.

The holotype strain of Nemania primolutea ( HAST 91102001 ) was isolated from a dead trunk of Artocarpus communis ( Ju et al. 2005) . According to the phylogram (Fig. 9 ), our strain was identified as Nemania primolutea with 94 % ML and 1.00 PP support. The isolate MFLUCC 24-0507 shares a similar asexual morph with HAST 91102001 . However, our strain differs from HAST 91102001 in having larger conidia (2.4–4.5 µm × 5.5–8.4 µm in MFLUCC 24-0507 vs. 2.5–3 × 4.5–6.5 in HAST 91102001 ). This species has also been reported from Lagerstroemia sp. in Louisiana, USA ( Garcia-Aroca et al. 2021), and Ramalina peruviana (lichen) in the Philippines ( Galinato et al. 2021). However, a lack of micro-morphology and insufficient molecular data were provided to verify the species identification. In this study, we illustrated Nemania primolutea on rice as a new host record.

Figures

Figure 8. Nemania primolutea (MFLUCC 24-0507, new host record). a. Front and reverse of the colony on PDA; b, c. Conidiophores and conidia; d. Branched conidiophores; e. Conidia. Scale bars: 20 μm (b, c); 10 μm (d, e).

Figure 9. Phylogram of ML analysis based on combined ITS, LSU, rpb 2, and tub 2 sequence data. ML bootstrap support values equal to or higher than 60 % and Bayesian probability values (PP) equal to or above 0.80 are given at the nodes (ML / PP). The tree is rooted to Rosellinia britannica (MFLU 17-302). The isolate from the current study is highlighted in red, and type strains are indicated in bold black.