Lecanoropsis subintricata (Nyl.) Ivanovich & Printzen, 2025

Ivanovich, Cristóbal, Weber, Lilith, Palice, Zdeněk, Hollinger, Jason, Otte, Volker, Sohrabi, Mohammad, Sheehy, Steve & Printzen, Christian, 2025, A taxonomic revision of the lichen genus Lecanoropsis (Lecanoraceae), Phytotaxa 695 (1), pp. 1-56 : 45-47

Lecanoropsis subintricata (Nyl.) Ivanovich & Printzen , comb. nov. Fig. 14A & B , 17D & E .

MycoBank nº: 847157

Basionym: Lecanora varia var. subintricata Nyl. , Flora 51: 478 (1868).

Type: — RUSSIA: Kola, super corticem Betulae, 1863, Fellman, Lich. Arct. Coll. 133) (H-NYL 26661= H 9506877!— holotype).

Synonyms: Lecanora subintricata (Nyl.) Th. Fries (1871: 265) .

Lecanoropsis subintricata (Nyl.) M. Choisy (1949: 143) , nom. illeg. (see Notes under Lecanoropsis in p. 14).

Thallus often endosubstratal, rarely episubstratal. When episubstratal, thallus effuse and amorphous, whitish grey to grey with or without a yellow hue, rarely brown or dark brown. Photobiont a chlorococcoid/trebouxoid alga. Apothecia rounded, usually single or in small groups, appressed to sessile, (0.24–)0.34–0.61(–0.63) mm in diameter. Apothecial disc weakly concave to flat, rarely weakly convex, typically pale grey to greyish, more rarely pale yellow to beige or ochre, darkening into dark grey, matte to rarely glossy in older apothecia, epruinose to occasionally finely to coarsely white pruinose. Apothecial margin prominent, usually persistent but sometimes disappearing in older, convex apothecia, (0.03–)0.05–0.08 (–0.1) mm wide. Pale yellow to yellowish beige. Amphithecium rarely with golden-dark brown granules, which are mostly restricted to the algal layer or the outer edge of the cortex. Amphithecial cortex thick, more or less even, heavily gelatinized (19–)25–50(–58) µm wide laterally, (19–)29–48(–59) µm wide basally, hyaline. Parathecium indistinct. Epihymenium hyaline or pale to golden-brown or brown, some specimens with greenish spots, due to a mixture of Cinereorufa green and Superba brown, sometimes with an epipsamma of golden-brown granules, rarely streaking into the hymenium. Hymenium hyaline, (30–)35–45(–50) µm high. Subhymenial layers hyaline, (35–)50–145(–175) µm high. Paraphyses simple, rarely branching and anastomosing, ca. 1.5–2 µm wide, apices weakly capitate, ca. 2–3.5 µm wide, apical gel sheath ca. 2.5–3.5 µm wide. Spores ellipsoid, simple, hyaline, (5.0–)6.3–9.6(– 12.0) × (2.5–)3.2–4.2(–6.0) µm. Conidia : Microconidia long-bacilliform, 6–7 × 1–1.5 µm; mesoconidia ellipsoid, short-cylindrical, 2.8–3.3 × 2–2.5 µm ( van den Boom & Brand, 2008); macroconidia and leptoconidia conidia were not found.

Chemistry: Usnic acid. Brialmontin 1 was reported by van den Boom & Brand (2008), and isousnic acid by Printzen (2001). Only usnic acid was detected in our samples by TLC.

Substrate: Usually on wood (rarely bark) of Picea , Quercus and Betula . Also, on worked wood such as fence posts.

Ecology: In subalpine to montane forest. Also found in subarctic biomes between 740–2300 m alt. Printzen (2001) reports specimens collected at elevations up to 3500 m alt. in Arizona and New Mexico, U.S.A.

Distribution: Circumboreal, found in central Europe, Fennoscandia, the Caucasus region and Japan. Also present in the western U.S.A.

Notes: Lecanoropsis subintricata is characterized by its uniformly rounded, usually flat apothecia with prominent, smooth, yellowish margins and the presence of usnic acid. Extreme phenotypes of L. subintricata , however, may possess a thin and discontinuous margin, and a weakly convex ochre to brownish disc. These morphs may be confused with L. sarcopidoides , which sometimes grows intermixed with L. subintricata , but L. sarcopidoides produces pseudoplacodiolic acid, while L. subintricata produces usnic acid. In addition, L. sarcopidoides has a whitish rather than yellowish margin that is mostly poorly developed, of ragged appearance and often disappearing with age. Apothecial discs of L. subintricata have a finely granulose pruina or are epruinose, whereas those of L. sarcopidoides usually have a coarse whitish pruina if the apothecia are not too old.

Selected specimens studied: AUSTRIA. Salzburg: Tamsweg , ca. 1900 m alt., 47°12.0148’ N 13°51.73442’ E, 01 September 2019, C. Ivanovich & L. Weber LUN19 (FR-0362786), Lecanomics Code: 505 ; ca. 1700 m alt., 47° 10.1670’ N 13°54.8289’ E, 02 September 2019, C. Ivanovich & L. Weber LUN26 (FR-0362787), Lecanomics Code: 507 ; CZECH REPUBLIC. Jihočeský kraj: Prachatice , 1248 m, 48°46.4784’ N 13°51.4217’ E, 07 July 2017, Z. Palice 21287 (FR-0362788; PRA), Lecanomics Code: 236 ; Stožec, Černý Kříž , 740 m alt., 48°51.6647’ N 13°51.5071’ E, 08 August 2017, Z. Palice 14449, 14450 & C. Printzen (FR-0261121, FR-0261122; PRA), Lecanomics Code: 237, 11 ; 740 m alt., 48°51.6681’ N 13°51.5168’ E, 14 July 2016, Z. Palice 21295 (FR-0362780; PRA), Lecanomics Code: 243 ; FINLAND. Enontekiö: Kilpisjärvi , 550 m alt., 69º 3.8602 N, 20º 45.1013 E, L. Weber 13–2021 01.08.2021 ( H) Lecanomics code 1496 ; GERMANY. Bayern: Freyung-Grafenau, Nationalpark Bayerischer Wald , 1091 m alt., 48°56.78’ N 13°28.0506’ E, 26 August 2017, S. Kern TS3-7 - 7 (FR-0279002) Lecanomics Code: 201 ; 1078 m alt., 48°56.7421’ N 13°28.1429’ E, 08 December 2017, S. Kern TS3-38 - 6 (FR-0362790), Lecanomics Code: 209 ; 1103 m alt., 48°56.7652’ N 13°28.1377’ E, 08 December 2017, S. Kern TS3-52 - 6 (FR-0362791), Lecanomics Code: 216 ; 1096 m alt., 48°56.7611’ N 13°28.1754’ E, 08 December 2017, S. Kern TS3-47 - 14 (FR-0362792), Lecanomics Code: 210 ; 1089 m alt., 48°56.7605’ N 13°28.1591’ E, 02 September 2017, S. Kern TS3-55 - 6 (FR-0362793), Lecanomics Code: 218 ; 1092 m alt., 48°56.803’ N 13° 28.1030’ E, 28 August 2017, S. Kern TS4-24 - 5 (FR-0362794), Lecanomics Code: 229 ; 1028 m alt., 48°56.351’ N 13º 28.6590’E, 08 September 2017, S. Kern TS4-51 - 6 (FR-0362795), Lecanomics Code: 230 ; JAPAN. Gunma Prefecture: Katashina-mura, Nikko National Park , 1760 m alt., 36°49.2521’ N 139°22.5740’ E, 02 October 2019, C. Printzen 15562 (FR-0267116), Lecanomics Code: 604 ; Tochigi Prefecture: Nikko City administrative region, Nikko National Park , 2300 m alt., 36°46.2149’ N 139°29.6080’ E, 01 October 2019, C. Printzen 15515 (FR-0267112), Lecanomics Code: 600 ; RUSSIA. Krasnodar Krai: Maykopsky District , 2070 m alt., 43°58.3498’ N 40°28.1836’ E, 13 September 2018, V. Otte 54753 ( GLM) Lecanomics Code: 292 ; 43° 58.3498’ N 40° 28.1836’ E, 13 September 2018, V. Otte 54754 ( GLM) Lecanomics Code: 293 ; SWEDEN. Dalarna: Särna , 900 m alt., 62°2.0102’ N 13°12.7076’ E, 16 October 2015, J. Hermansson 19599 ( UPS L-767683), Lecanomics Code: 258 ; 920 m alt., 62°2.046’ N 13°12.54’ E, 16 October 2015, J. Hermansson 19606 ( UPS L-767688), Lecanomics Code: 263 ; U.S.A. Nevada: Lincoln Co., 2200 m alt., 37°33.0’ N 115°45.6’ W, 26 May 2016, J. Hollinger 12126a (FR-0362798), Lecanomics Code: 976 ; 2325 m alt., 37°54.18’ N 114°33.6’ W, 02 June 2016, J. Hollinger 12721 (FR-0362799), Lecanomics Code: 980 ; 2210 m alt., 37°27.402’ N 114°26.814’ W, 03 June 2018, J. Hollinger 21739 (FR-0362801), Lecanomics Code: 999 ; 2540 m alt., 37°58.74’ N 115°6.24’ W, 03 August 2019, J. Hollinger 22817 (FR-0362802), Lecanomics Code: 1002 ; Utah: Garfield Co., 2650 m alt., 37°49.44’ N 111°53.94’ W, 05 May 2017, J. Hollinger 17296 (FR-0362803), Lecanomics Code: 989 .

Figures

FIGURE 14. Lecanoropsis subintricata (A & B), L. sarcopidoides (C & D) and L. subcinctula (E & F).A, Germany, Bayern, S. Kern TS3- 7-7 (FR-0279002; L201), habitus. B, Czech Republic, Jihočeský kraj, Z. Palice 21295 (FR-0362780; L243), apothecial section in LCB. C, Czech Republic, Jihočeský kraj, Z. Palice 27894 (FR-0362781; L574). D, Slovakia, Banskobystrický kraj, A. Guttová, J. Halda & Z. Palice 27751 (FR-0362785; L565), apothecial section in LCB. E, Japan, Shizuoka Prefecture, E.Almquist s.n. (H-NYL 26656=H9506878, lectotype), habitus. F, Japan, Tochigi Prefecture, C. Printzen 15192 (FR-0267107; L595), apothecial section in LCB. Scales: A, C & E = 500 µm; B, D & F = 20 µm.

FIGURE 17. Leptoconidia (A & B), Microconidia (C & D) and Mesoconidia (E). Lecanoropsis albellula (A), L. saligna (B). L. pseudosarcopidoides (C) and L. subintricata (D & E). A, Austria, Salzburg, C. Ivanovich & L. Weber LUN25 (FR-0362754; L506). B, Germany, Bayern, S. Kern TS3-12-22 (FR-0183022; L203). C, Italy, Trentino, P. van den Boom 10793 (B600187274, holotype). D, Austria, Salzburg, C. Ivanovich & L. Weber LUN26 (FR-0362787; L507). E, same specimen as D. All conidia were stained with Phloxine B. Scales: A–F = 10 µm.