Holocentropus varangensis Mey, 1987

Wiberg-Larsen, Peter, Andersen, Trond, Elven, Hallvard & Salokannel, Juha, 2024, Holocentropus varangensis Mey, 1987, a probable hybrid between Holocentropus insignis Martynov, 1924 and H. picicornis (Stephens, 1836) (Trichoptera, Polycentropodidae), Norwegian Journal of Entomology 1987 (1), pp. 1-7 : 2-4

publication ID

https://doi.org/10.61698/nje/72-1-001

persistent identifier

https://treatment.plazi.org/id/03DDA24C-FFD6-BB7F-FD3C-FD77FF390CE7

treatment provided by

Felipe

scientific name

Holocentropus varangensis Mey, 1987
status

 

Holocentropus varangensis Mey, 1987 View in CoL

NORWAY, FI, Sør-Varanger : Varanger Peninsula, lake inlet at tributary 3 of Gjøkvatn, 10 July 1969, 1♂ (holotype), leg. W. Tobias (the genitalia of the holotype are shown in Figure 3A– C View FIGURES 1–3 ) .

Notes: The holotype is housed in the Senckenberg Museum of Natural History, Görlitz, Germany - not in the Museum of Natural History, Berlin, as stated in Mey (1987). The specimen was originally labelled “ H. insignis ” by Wolfgang Tobias. The abdomen including the genitalia was already cleared in KOH by Mey. The holotype was made available for our study by the Senckenberg Museum of Natural History , Görlitz .

DNA sequencing

With permission from Senckenberg Museum, we attempted to obtain a DNA barcode from one leg of the holotype of H. varangensis . The leg was processed non-destructively, i.e. securing conservation of the exoskeleton. The aim was to obtain a barcode sequence of the mitochondrial cytochrome oxidase subunit I gene ( COI) to be compared with similar available sequences for H. insignis and H. picicornis (see Boonstra et al. 2018). As the mitochondrial DNA is maternally inherited, the barcode of H. varangensis - if this is a hybrid - should be identical to that of one the potential mothers, either H. insignis or H. picicornis . This would strongly support the hybridization hypothesis.

The extraction was carried out by courtesy of the Ecology and Genetics Research Unit, Oulu. The sample was then analyzed (28 April 2022) at the Canadian Centre for DNA Barcoding ( CCDB) (Plate: CCDB-40752; Process-id: LEFIJ28025-22 ). It was then subjected to the “ NGSFT ” protocol as explained in Prosser et al. (2016) .

COI

University of Coimbra Botany Department

CCDB

Crustacean Collection of the Department of Biology

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