Gilmaniella multiporosa Moustafa & Ezz-Eldin., Mycol.

Sánchez, Romina M. & Bianchinotti, M. Virginia, 2023, New records of mitosporic ascomycetes on Nothofagaceae from Patagonia, Argentina, Boletin de la Sociedad Argentina de Botanica 58 (4), pp. 491-503 : 495-497

4. Gilmaniella multiporosa Moustafa & Ezz-Eldin., Mycol. Res . 92: 502. 1989. Fig. 2 E-I.

Colonies sporodochial, punctiform, irregular, gregarious, sometimes confluent, dark brown, bright. Mycelium immerse, ramified, pale brown, individual cells cylindrical to reniform, smooth, 4-10 × 3-4 µm (M = 6.9 × 3.3). Conidiophores macronematous, cylindrical, septate, pale brown, 33-93 × 2-3 µm (M = 60.4 × 2.9). Conidiogenous cells mono or polyblastic, clavate, discrete, terminal or intercalar, smooth, pale brown, 6-18 × 3-7 µm (M = 9.9 × 4.7). Conidia holoblastic, in chains of up to 8 conidia, subspherical, smooth, pale brown, 6-9 × 4-7 µm (M = 7.7 × 6.3; n = 23), surface with many scars or pores, from which new lateral chains may be produced; terminal conidia globose, smooth, dark brown, 9-17 × 9-14 (M = 13.8 × 12; n = 31).

Distribution and habitat. Growing on bark of N. antarctica in Argentina and in saline soil in Egypt ( Moustafa & Ezz-Eldin, 1989).

Comments. The specimen found in Patagonia could not be isolated, however diagnostic morphological features such as shape and size of conidiogenous cells and conidia fit well with those materials isolated from the soil of a salt marsh at north of Sinai ( Egypt). The patagonian collection differs only in possessing conidiophores and having longer conidial chains (up to 8 conidia) than in Egyptian specimens (2-3 conidia) ( Moustafa & Ezz-Eldin, 1989).

Material examined. ARGENTINA. Neuquén Prov.: Parque Nacional Nahuel Huapi, National Route 40, going from Villa La Angostura to Villa Traful , 1 km before to the provincial access Route 65 (40° 37’ 1.358” S, 71° 38’ 48.098” W), 16-V-2007, on bark of N. Antarctica, Bianchinotti and Sánchez 564 ( BBB) .

Figures

Fig. 2. Dwiroopa ramya. A: Stromatic conidioma on decorticated wood. B: Two microconidia surrounded by macroconidia. C: View of the longitudinal germ slits of macroconidia. D: Conidium germinating through the longitudinal slit. Gilmaniella multiporosa. E: Sporodochial conidioma on substrate. F: Conidiophore. G: Conidiogenous cell. H: Conidia in chains. I: Terminal conidia showing multiple scars. Monodictys paradoxa. J: Conidioma on substrate. K: Conidium detached from the conidiogenous cell which narrows towards the apex. L: Conidium showing the lighter basal cells and a rest of the conidiogenous cell. M: Germinating conidium and formation of a new one. N: Different shapes and sizes of conidia. Pycnopeziza quisquiliaris. O: Vertical section of a conidioma. P: Chain of two conidia (phloxine stain). Q: Conidia attached to conidiogenous cells (phloxine stain). R: Conidiophore.Abbreviations= cg: conidiogenous cell; cp: conidiophore; mc: microconidia; s: scars; sl: germ slits. Scale bars= A: 1 mm; B–I, L–N, P: 15 µm; K, Q–R: 10 µm; O: 50 µm.