Diplodoma giulioregenii La Cava & Scalercio, 2025

Cava, Sara La, Rijllo, Giuseppe, Zucco, Giada & Scalercio, Stefano, 2025, Revisiting the genus Diplodoma Zeller, 1852 in Europe: DNA barcoding reveals the presence of an undescribed species from forested habitats of southern Italy (Lepidoptera: Psychidae), Zootaxa 5583 (2), pp. 371-382 : 373-376

publication ID	https://doi.org/10.11646/zootaxa.5583.2.8
publication LSID	lsid:zoobank.org:pub:46B86E84-20B9-49BD-B241-D2159025F8A2
DOI	https://doi.org/10.5281/zenodo.14812933
persistent identifier	https://treatment.plazi.org/id/03C0BA2C-2064-3D15-3BD3-CF95FE25FE18
treatment provided by	Plazi
scientific name	Diplodoma giulioregenii La Cava & Scalercio
status	sp. nov.

Treatment

Diplodoma giulioregenii La Cava & Scalercio , sp. nov.

http://zoobank.org/ urn:lsid:zoobank.org:pub:46B86E84-20B9-49BD-B241-D2159025F8A2

( Figs. 1–19 View FIGURE 1 View FIGURES 2–4 View FIGURES 5–9 View FIGURES 10–17 View FIGURE 18 View FIGURE 19 )

Type material. Holotype. [ ITALIA] ♂, CALABRIA,— CC _ C2 , V.ne Argentino, Montalto Uff., 565 m — 1.VI.2016, 39.4082°, 16.1209°, Scalercio & Infusino leg. [DNA barcode specimen ID LEP-SS-04527] . Paratypes. [ ITALIA] 1♂, CALABRIA SL_AF, Vallone Tasso, Spezzano Sila ( CS), 1402 m — 9.VII.2018, 39.332823°, 16.414273°, Scalercio S. leg. [DNA barcode specimen ID LEP-SS-04552]; Gen. prep. CREA —0279, Stefano Scalercio ; 1♂, ITALIA — CALABRIA SL_ AM, Vallone Tasso, Spezzano Sila ( CS), 1376 m — 16.VII.2018, 39.332393°, 16.418499°, Scalercio S. leg. [DNA barcode specimen ID LEP-SS-04553], Gen. prep. CREA —0266, Stefano Scalercio .

Diagnosis. Male antenna composed by about 35 antennomers as in D. taurica , while in D. laichartingella is composed by no more than 30 antennomers. Intercalary cell presents as in D. laichartingella , lacking in D. taurica . In male genitalia ( Figs. 5 View FIGURES 5–9 , 10 View FIGURES 10–17 ) the length-to-width ratio (L-W ratio) of clasper is significantly higher in D. giulioregenii (N=2; L-W ratio ranging from 1.8 to 2.5) than in available dissected males of D. laichartingella (N=9; L-W ratio min=0.7; mean=1.0±0.2; max=1.3) ( Figs. 11–17 View FIGURES 10–17 ) and is similar to the picture of the original description of D. taurica (LW ratio =1.9). Tendon 35 degrees angled upward at about half of its length in D. giulioregenii ( Fig.18a View FIGURE 18 ), straight in D. taurica , ( Fig. 18b View FIGURE 18 ) and mild curved upward in D. laichartingella ( Fig.18c View FIGURE 18 ). Despite the worn appearance of types, D. giulioregenii does not seem to be significantly different from D. laichartingella in terms of wing pattern and wingspan, whereas D. taurica is significantly smaller ( Arnscheid & Weidlich, 2017).

Descriptions

External characters ( Figs. 1–4 View FIGURE 1 View FIGURES 2–4 ).Adult male, holotype ( Fig. 1 View FIGURE 1 ): wingspan 12 mm; colouration and vestiture: head ( Fig. 2 View FIGURES 2–4 ) covered in light-brown, shiny, short hairs on frons, with longer light-brown hairs posterior to antennal sockets; labial palpi ( Fig. 3 View FIGURES 2–4 , left side (a) and top view (b)) elongated and densely covered with long scales. Round compound eye about 320µm heigh; distance between eyes about 450µm; a well-developed ocellus present immediately above. The antenna ( Fig. 4 View FIGURES 2–4 ) thread-like, approximately half-length of the costa, composed by about 35–38 antennomers; dorsally covered by scales and ventrally ciliated with cilia in average 115µm long. The thorax is covered with shiny light-brown hairs. The forewings are elongated and darkish, with shiny light spots that are visible on coastal margin and with shiny yellowish scales visible in the inner margin. Cloaking scales of forewings belong to IV class ( Sauter, 1956). The fringe scales are short and compact, with shiny light spots. Venation with 10 veins from discal cell; intercalary and accessory cells present. The hindwings are uniformly brownish, with a few shiny light spots on the proximal margin of the wings. Venation with 6 veins from discal cell. The fringe scales are longer near the proximal margin of the hindwings and shorter toward the distal margin.

Adult female. unknown.

Male genitalia ( Figs. 5–9 View FIGURES 5–9 ). Valva short and distinctly sclerotized, narrower distally with rounded apex, densely covered with short hairs ( Fig. 5 View FIGURES 5–9 ). The tendon is short and angled upward by 35 degrees at half of its ventral length. Sacculus long about two-thirds of valva length, roundish caudally with clasper narrower distally, distinctly pointed, and sclerotized. Vinculum and tegumen fused. Pointed saccus 295µm long and 56µm wide at half of its length ( Fig. 6 View FIGURES 5–9 ). Slightly oval tegumen, distally with two hump shaped appendages ( Fig. 7 View FIGURES 5–9 ). Phallus 708µm long, thin slightly curved and broader caudally ( Fig. 8 View FIGURES 5–9 ), bearing distally 4 tooths that become smaller proximally ( Fig. 9 View FIGURES 5–9 ).

Case. unknown.

Variation. we cannot observe variation in wings pattern because our specimens are worn due to the collecting method. The only observable variation is in wingspan, which ranges between 12 mm and 13 mm (n=3) ( Fig. 1 View FIGURE 1 , 19 View FIGURE 19 ), and in phallus length ranging 684 and 708µm in paratypes.

Genetic data. the maximum intra-BIN difference is 0.8% with the two paratype identical to each other. The intra-BIN average distance is 0.54% (n=3). The distance from the nearest BIN (BOLD:AAF5091) is 6.25%, and it is composed by European sequences of Diplodoma laichartingella . The p-distance from the Nearest Member calculated by the BOLD Identification System is 6.76% for the holotype and 6.45% for the paratypes. The Nearest Member is a specimen from Norway (Sample ID: NHMO-DAR-12513). In the neighbor-joining tree ( Fig. 20 View FIGURE 20 ) resulting from the analyses of available DNA barcoding sequences belonging to the genus Diplodoma , eight distinct BINs were present across the European continent and one in Far East Asia. Phylogenetic analysis showed that the cluster of Diplodoma giulioregenii separates earlier than the branches containing D. laichartingella and D. adspersella .

Diagnostic SNPs. we report the 679 bp long DNA barcoding sequence of the holotype compared with the Diplodoma sequences utilised in this paper, with the 23 diagnostic SNPs (Single Nucleotide Polymorphism) evidenced in bold:

TTTATATTTTATTTTAGG T ATTTGA T C G GGAATAATTGGAACATCTTTAAGATT A TTAATTCGAGTAGAAT TAGG G ATTCCTAATTCATTTCTTGGAAGAGATCAAATTTATAATACTATTGTAAC T GCTCATGCCCTTATT ATAATTTTTTTTATAGTTATACCTATTATAATTGG G GGATTTGGAAATTGATTAGTACCTTTAATATTGGGG GCCCCTGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGACTTCTTCC A CCTTCTTTAATAATTTT AATTATAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAAC A ATTTA T CCTCC CC T T TCTTCTA ATTTAACCCATTCAGGAAGTTCAGTTGATTTAGCAATTTTTTCTTTACATTTAGCAGGAATTTCATCTAT TTTAGGGGCAGTAAATTTTATTACAACAATTATTAATATACGACCATTTAA C ATATC A TTAGATCAAATAC C C TTATTTGTATG G TCTGTGGCTATTACTGCAGTA C T T TTACTTTTATCTTTACCAGTTTTAGCTGGAGC AATTACTATGTTATTAAC C GATCGAAATTTAAATACATC G TTTTTTGATCCTGCTGGAGGTGGAGA C CCT ATTTTATTCCAACATTTATTTTGATTTTTTGGTCACCCTGAAGTT.

Biology: unknown.

Distribution: endemic of South Italy, with type specimens collected from two different mountainous areas, the Catena Costiera Paolana and the Sila Massif, at a distance of 27 km.

Habitat: all specimens were found in forested habitat, the holotype in a dense chestnut woodlot and the paratypes in a pure beech forest and in a mixed beech-Calabrian black pine forest.

Derivatio nominis: in memory of a young Italian researcher murdered by all the evil in the world, waiting for the truth for Giulio Regeni.