Dactylaria hwasunensis H. Liu & H. Sang, 2025

Liu, Haifeng, Choi, Hyeongju, Paul, Narayan Chandra, Ariyawansa, Hiran A. & Sang, Hyunkyu, 2025, Discovering fungal communities in roots of Zoysia japonica and characterising novel species and their antifungal activities, IMA Fungus 16, pp. e 138479-e 138479 : e138479-

Dactylaria hwasunensis H. Liu & H. Sang sp. nov.

Fig. 10

Etymology.

Name refers to Hwasun County in Korea, where it was isolated.

Description from living culture CMML 20-35 .

Sexual morph: undetermined. Asexual morph: Sporulation abundant on MEA. Conidiophore erect, mironematous to macronematous, aseptate or septate, hyaline, 6–35 μm in length, 2.2–2.8 μm in width. Conidiogenous cells terminal, integrated, hyaline 2–2.8 μm wide. Conidia clavate, hyaline, blunt end, 1–5 septate, 10–60 × 2.2–2.8 μm (Fig. 10 b – h ).

Type.

Korea • South Jeolla Province, Hwasun , isolated from roots of Zoysia japonica , October 2020, H. Liu and H. Sang, holotype CMML 20-35 H (permanently preserved in a metabolically inactive state), ex-holotype CMML 20-35 , ex-isotype CMML 20-88 .

Culture characteristics.

Colony reaching 31.81 mm diam. after 7 days in darkness at 25 ° C on PDA, white to yellowish, surface smooth, cracked (Fig. 10 a ).

Notes.

In phylogenetic analysis of genus Dactylaria using sequence data of LSU, the strains used in the present study CMML 20-35 and CMML 20-88 formed a distinct clade sister to clade containing representative strain of D. fragilis (P 057) and ex-type strain of D. acaciae ( CPC 29771 ) with a high statistical support (84 % / 0.95) (Fig. 11 ). Based on nucleotide sequences, ex-holotype strain of D. hwasunensis ( CMML 20-35 ) differed from ex-type strain of D. acaciae ( CPC 29771 ): LSU sequence identities = 806 / 818 (98.53 %). D. hwasunensis ( CMML 20-35 ) also differed from representative strain of D. fragilis (P 057): LSU sequence identities = 844 / 857 (98.48 %). Morphologically, conidial dimensions of D. hwasunensis are larger than D. acaciae (16–37 × 2–2.5 μm; Crous et al. (2016)) and D. fragilis (18–26 × 1.5 µm; De Hoog (1985)). Therefore, Dactylaria hwasunensis sp. nov. was introduced in this study to accommodate CMML 20-35 and CMML 20-88 in the genus Dactylaria .

Figures

Figure 10. Dactylaria hwasunensis sp. nov. (CMML 20-35) a front and reverse sides of colony on PDA b – g conidiophores and conidia h conidia. Scale bars: 10 μm.

Figure 11. Maximum Likelihood phylogenetic tree, based on LSU sequences from Dactylaria species. Bootstrap values (BS) and Bayesian posterior probabilities (PP) are given at the nodes (BS / PP). Strains obtained from this study are in bold blue. Ex-type isolates are marked with T. Ramularia endophylla (CBS 113265) was used as the outgroup taxon.