Chionosphaera foliicola C. Y. Chai & F. L. Hui, 2025

Wang, Peng, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Two new species of Chionosphaera and Kurtzmanomyces (Chionosphaeraceae, Agaricostilbomycetes) isolated from China, MycoKeys 125, pp. 81-94 : 81-94

Chionosphaera foliicola C. Y. Chai & F. L. Hui sp. nov.

Fig. 2

Etymology.

The specific epithet foliicola refers to the ex-type strain isolated from a leaf.

Typus.

China • Hainan Prov.: Wuzhishan City, Wuzhi Mountain , in the phylloplane of Symplocos adenophylla , August 2024, S. L. Lv, NYNU 248111 ( holotype GDMCC 2.531 T preserved as a metabolically inactive state, culture ex-type PYCC 10141 ) .

Description.

On YM agar after 7 days at 25 ° C, the streak culture is pale orange tough, smooth and glossy with an entire margin. In YM broth after 3 days at 25 ° C, cells are ellipsoidal to cylindrical (2.7–6.0 × 4.1–10.5 µm) and occur singly or in pairs. Budding is polar through minute stalks, normally one to two per cell. After a month in YM broth at 20 ° C, a sediment is formed, but no ring and pellicle are observed. After 2 weeks in Dalmau plate culture on CMA at 20 ° C, hyphae were observed. Sexual structures are not observed on PDA, CMA or V 8 agar at 17 ° C. Ballistoconidia are not produced. Sugar fermentation is absent. The following carbon sources are assimilated: glucose, inulin (delayed), sucrose, D-galactose (delayed), lactose (weak and delayed), trehalose, maltose, melezitose, methyl α-D-glucoside (delayed), cellobiose, salicin (weak), L-sorbose (weak and delayed), L-rhamnose (weak and delayed), D-xylose (weak and delayed), L-arabinose (weak and delayed), D-arabinose (weak), 5 - keto-D-gluconate, ethanol (weak), glycerol (weak and delayed), ribitol (weak), galactitol, D-mannitol, D-glucitol (weak and delayed), DL-lactate (weak and delayed), succinate, citrate (weak and delayed), and d-glucono- 1, 5 - lactone. Raffinose, melibiose, d-ribose, methanol, erythritol, myo - inositol, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-d-gluconate, and D-glucuronate are not assimilated. L-Lysine is assimilated as sole nitrogen sources. Nitrate, nitrite, ethylamine, and cadaverine are not assimilated. Growth is observed at 30 ° C, but not at 35 ° C. Growth in the presence of 0.01 % cycloheximide, 10 % NaCl plus 5 % glucose as well as 1 % acetic acid is negative. Growth in the vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. There is no hydrolysis of urea and no starch formation. The DBB reaction is positive.

Additional strain examined.

China • Hainan Prov.: Wuzhishan City, Wuzhi Mountain , in the phylloplane of Symplocos adenophylla , August 2024, S. L. Lv, NYNU 248157 .

GenBank accession numbers.

Holotype GDMCC 2.531 T ( ITS: PV 770167 , LSU: PQ 571723 ; tef 1 α: PX 046189 ); additional strain NYNU 248157 ( ITS: PV 774782 , LSU: PV 774781 ; tef 1 α: PX 046190 ).

Note.

Physiologically, C. foliicola sp. nov. differs from the closely related species, C. apobasidialis , C. cuniculicola , and C. pinicorticola , in its ability to assimilate sucrose and lactose (Table 2 ).

Figures

Figure 2. Morphology of Chionosphaera foliicola (NYNU 248111). A. Colony on YM agar after 7 days at 20 ° C; B. Budding cells in YM broth after 3 days at 20 ° C.