Aspergillus brunneoviolaceus Bat. & H. Maia

Harikrishnan, Kuniyil, Rajeshkumar, Kunhiraman C., Patil, Ravindra M., Jeewon, Rajesh & Visagie, Cobus M., 2025, Aspergillus dhakephalkarii and A. patriciawiltshireae spp. nov., two new species in Aspergillus sect. Nigri ser. Japonici (Eurotiales, Aspergillaceae) from India, Phytotaxa 695 (1), pp. 57-79 : 74

Aspergillus brunneoviolaceus Bat. & H. Maia , Anais Soc. Biol. Pernambuco 13: 91 (1955) ( Figure 8 )

MycoBank No. MB292838

Classification: Aspergillus subgen. Circumdati sect. Nigri ser. Japonici .

Description: Colony diam (25 ºC, 7 d, in mm): CREA 26–30; CYA>70; CYA 30˚C>70; CYA 37˚C 12–26; CY20S 64–68; CZ 55–57; DG18 26–30; MEA 60–65; MEA 30˚C 66–68; MEA 37˚C 2–3; MEAbl 50–55; M20S;>70; OA 60; YES>70.

Colony characters (25 ˚C 7 d): CYA: Colony surface velutinous; margin irregular; mycelium white (1A1); sporulation dense, brown (6E4); exudates and soluble pigments absent; reverse pale yellow (4A3) at centre, white (1A1) at margin. CYA20S: Colony surface velutinous; margin irregular; mycelium white (1A1); sporulation dense, brown (7E4); exudates and soluble pigments absent; reverse white (1A1). CZ: Colony surface velutinous, mycelium white (1A1); sporulation absent; exudates and soluble pigments absent; reverse hyaline. DG18: Colony surface velutinous, mycelium white (1A1); sporulation absent; exudates and soluble pigments absent; reverse hyaline. MEA : Colony surface velutinous; margin irregular; mycelium white (1A1); sporulation high, brown (7E4); exudates and soluble pigments absent; reverse yellowish white (4A2). MEAbl: Colony surface velutinous; margin irregular; mycelium white (1A1); sporulation high, brown (7E4); exudates and soluble pigments absent; reverse yellowish white (4A2). M20S: Colony surface velutinous; margin irregular; mycelium white (1A1); sporulation high, greyish brown (7E4); exudates and soluble pigments absent; reverse yellowish white (4A2). OA: Colony surface velutinous, margin irregular; mycelium white (1A1); sporulation moderate, dark brown (8F4); reverse white (1A1). YES: Colony surface velutinous, irregularly sulcate, margin irregular; mycelium white (1A1); sporulation moderate, brown (5F4); reverse orange-white (5A2). CREA : Colonies have weak growth, and acid production is absent.

Conidiophores radiate, splitting into poorly defined columns; Stipes (192–)200–557 × 10–14 µm, smooth, hyaline. Vesicles 28–46 µm diam globose to ellipsoidal, uniseriate. Phialides 6–9 × 3.5– 4 µm flask-shaped and cover the entire surface of the vesicle, covering entire vesicle. Conidia 3.5–5 × 3–4 µm, globose to ellipsoidal, echinulate surface.

Material examined: INDIA, Maharashtra, Velhe-Mangaon, 18.325833°N, 73.521667°E, from soil, 10 August 2023, Harikrishnan K, Ravi Patil & Rajeshkumar KC ( AMH 10693, NFCCI 5752)

Notes: Phylogenetic analyses resolved our strain with others of A. brunneoviolaceus . Jurjević et al. (2012) observed acid production in A. brunneoviolaceus , but acid production on CREA is absent in NFCCI 5752.

Figures

FIGURE 8. Aspergillus brunneoviolaceous (NFCCI 5752): A. Colonies on CYA after 3 days at 25± 2 °C, B. Colonies on MEA after 3 days at 25± 2 °C, C. CREA obverse, D. CZ obverse, E. DG18 obverse, F. CYA20S obverse, G. OA (natural) obverse, H. YES obverse, I–L. Conidial heads (MEA), M. Conidiophore, N. Foot cell, O, P. Vesicle, Q. Conidia. Scale bars: I = 1mm. J, L = 200 µm. K = 100 µm. M–O = 20 µm. P, Q = 10 µm.