Apiospora mukdahanensis Monkai and Phookamsak

Absalan, Sahar, Armand, Alireza, Jayawardena, Ruvishika S., Suwannarach, Nakarin, Monkai, Jutamart, Jungkhun, Nootjarin, Lumyong, Saisamorn & Hyde, Kevin D., 2025, Endophytic xylariaceous fungi from rice in northern Thailand: discovery of novel species and new host records, MycoKeys 120, pp. 1-34 : 1-34

Apiospora mukdahanensis Monkai and Phookamsak , Diversity 14 (no. 918): 11 (2022)

Fig. 3

Description.

Endophytic from healthy panicle of Oryza sativa . Sexual morph: not observed. Asexual morph: Conidiophores 2.5–4 μm wide, basauxic, cylindrical, septate, straight or flexuous, sometimes reduced to conidiogenous cells, hyaline. Conidiogenous cells 7–21.5 × 4.5–6 μm (x ̄ = 13 × 5 µm, n = 10), cylindrical to subcylindrical, lageniform or ampulliform, pale brown to hyaline. Conidia 5–7 × 4–5.5 μm (x ̄ = 6.5 × 5 µm, n = 25), globose to subglobose, lenticular to side view with a pale longitudinal germ slit, thick-walled, brown to dark brown.

Culture characteristics.

Colonies on PDA reaching 46–48 mm in diameter after 7 days at 28 ° C, white, becoming pale orange with age, circular, floccose to cottony; reverse dull white.

Material examined.

Thailand • Chiang Rai Province, Mueang Chiang Rai District, Huai Sak Subdistrict , from healthy tissue part of rice panicle, 17 December 2021, Sahar Absalan (HS 88-2 b = MFLU 25-0030 ) (living culture MFLUCC 24-0511 ) .

GenBank numbers.

MFLUCC 24-0511 : ITS = PV 235259, tef 1 - α = PV 275673, tub 2 = N / A.

Notes.

Based on the morphological and molecular data, the isolate MFLUCC 24-0511 was identified as a representative of Apiospora mukdahanensis with 100 % ML and 1.00 PP bootstrap support (Fig. 2 ). Apiospora mukdahanensis was described by Monkai et al. (2022) and isolated from dead leaves of bamboo in Mukdahan Province, Thailand. Morphologically, our strain closely resembles the type strain MFLUCC 22-0056 . However, our observations revealed the presence of sterile cells, which were not reported in their collection ( Monkai et al. 2022). This study describes this species as a new host record from rice.

Figures

Figure 2. Phylogram of ML analysis based on combined ITS, tef 1 - α, and tub 2 sequence data. ML bootstrap support values equal to or higher than 60 % and Bayesian probability values (PP) equal to or above 0.80 are given at the nodes (ML / PP). The tree is rooted to Nigrospora gorlenkoana (CBS 480.73) and N. camelliae-sinensis (CGMCC 3.18125). The isolate from the current study is highlighted in red, and type strains are indicated in bold black.

Figure 3. Apiospora mukdahanensis (MFLUCC 24-0511, new host record). a. Front and reverse of the colony on PDA; b, c. Conidiomata formed on PDA; d. Conidiophores, conidiogenous cells, and conidia; e, f. Conidiogenous cells g. Sterile cell; h. Conidia. Scale bars: 20 μm (d); 10 μm (e – f); 5 μm (g – h).