Ruta museocanariensis Marrero Rodr., Vidal Matutano, Delgado Darias & Jaén Molina, 2023

Marrero-Rodríguez, Águedo, Vidal-Matutano, Paloma, Delgado-Darias, Teresa, Jaén-Molina, Ruth, Morales-Mateos, Jacob, Alberto-Barroso, Verónica & Velasco-Vázquez, Javier, 2023, Can material of a putatively extinct new species of Ruta (Rutaceae), preserved with mummies, provide new knowledge about evolution in the Canary Islands flora?, Willdenowia 53 (1), pp. 5-23 : 11-18

Ruta museocanariensis Marrero Rodr., Vidal Matutano, Delgado Darias & Jaén Molina , sp. nov. – Fig. 2F , 3F , 5 .

Holotype: from the shroud of infant mummy no. 49991 [ Fig. 1A ] in El Museo Canario, originating according to available data from sepulchral cave archaeological sites of Acusa ( Spain, Canary Islands, Gran Canaria, Artenara ), plant material extracted in February and March 2021 and March 2022 by T. Delgado, P. Vidal and Á. Marrero ( LPA 39791 [ Fig. 4A ]; isotype: LPA 39790 ) . – Paratypes: from the shroud of adult mummy no. 10 [ Fig. 1B ] in El Museo Canario, originating according to available data from sepulchral cave archaeological sites of Barranco de Guayadeque ( Spain, Canary Islands, Gran Canaria, Ingenio/ Agüimes ), plant material extracted in February and March 2021 by T. Delgado, P. Vidal and Á. Marrero ( LPA 39792 , LPA 39793 [ Fig. 4B ]) .

Typification — The four type specimens are physically deposited in the archaeological collection of El Museo Canario ( Las Palmas de Gran Canaria , Canary Islands, Spain) but are linked to and registered in the LPA herbarium (of the Jardín Botánico Canario Viera y Clavijo, Unidad Asociada al CSIC) by means of scanned copies or photographs and entry records. These are the oldest specimens among the collections of the LPA herbarium.

The material was extracted avoiding any additional damage to the mummies and their shrouds. Leafy branches and remains of infructescences with capsules were extracted from the shroud of the infant mummy EMC no. 49991 to assemble the holotype and isotype. The branches and leaves are very fragile and fragmented, but the capsules are well preserved (plant fruiting at the time of gathering) and offer more taxonomically valuable data. Leafy branches with inflorescences were extracted from the shroud of the adult mummy EMC no. 10 to assemble the two paratypes. This material is better preserved and less fragmented, but for taxonomic purposes it is less relevant because the inflorescences (plant flowering at the time of gathering) have deteriorated and do not include the most delicate parts such as petals or stamens. All the material was presumably originally collected from living plants by the indigenous population expressly for the preparation of the mummies’ shrouds.

Description — Shrubs; branches bearing subepidermal glands in form of black dots throughout plant; major branches erect-ascending, 22–40 cm long, 1.6–4(– 5) mm in visible basal diam.; terminal branches slender, leafy, 7–15.5 cm long, with (6–)8–15 alternate leaves. Petiole 1.7–4.1 cm long, 0.5–0.7 mm in diam., terete; leaf blade simple or 1-pinnatisect with a terminal pinna; middle leaves with 2(or 3) pairs of pinnae; basal cauline leaves simple or with 1 or 2 lateral pinnae, shorter than middle leaves; middle leaves long petiolate, 7.9–10.5 cm long including petiole, 4.6– 5.2(–7.3) cm wide, slightly fleshy, glabrous; upper leaves progressively smaller and simple; lateral pinnae generally opposite but occasionally alternate, linear-filiform, almost indistinguishable from rachis or petiole but flattened, 1.1–4.7 cm long, 0.5–1.6 mm wide; terminal pinna linear-filiform, 1.9–5.5 cm long, slightly longer than lateral pinnae; all conspicuously dotted with sub-crateriform dark glands, midvein always visible abaxially, somewhat concave adaxially, base terete, somewhat attenuate, apex rounded or more frequently pointed. Inflorescence terminal, paniculate-corymbose, 9–12 cm long, with 12–15 flowers; basal bracts like leaves but smaller and usually simple, linear-filiform, 1–2.5(–4) cm long, upper ones minute, subulate or triangular-subulate, 1–4 mm long; bracteoles triangular-subulate, 0.7–1.6 mm long, gland-dotted and with minute, stipitate glands toward base; peduncles variable, (1.2–)3.5–10.2(–20) mm long; pedicels 1.3–4.8 mm long, somewhat widening distally. Sepals with persistent, thickened, triangular or triangular-subulate lobes, 0.6–1.1 mm long, somewhat verrucose-glandular and with minute, stipitate glands. Petals and stamens unknown. Disk narrow, 0.4–0.9 mm long. Ovary globose-ovoid, c. 1 × 1 mm, verrucose, glabrous, with 4 deeply incised carpels. Fruits minute, dehiscent capsules, 4–5.5 mm in diam., opening along inner sutures, with 4 divaricate lobes forming a star shape, verrucose externally, glabrous. Seeds unknown.

Distribution — Ruta museocanariensis is known only from mummy shrouds from the sepulchral cave sites of Acusa (Artenara) and Barranco de Guayadeque (Ingenio/ Agüimes). Given the pre-Hispanic population’s lack of seafaring mobility between different islands of the archipelago ( Morales-Mateos 2010), and even less with the African mainland, there is no doubt that the species was collected on the Island of Gran Canaria, probably in the vicinity of the funerary sites. Moreover, there are several toponyms ( Fig. 6 ) in the surroundings of Acusa alluding to “ruda” (meaning Ruta ). The place names at Artenara (in the Barranco Hondo) are “La Ruda”, “Montaña la Ruda ” and “ Cañada de la Ruda”; in Tejeda (near Timagada and El Juncal) they are “Cuesta de la Ruda” and “Degollada de la Ruda”; and in El Carrizal (also in Tejeda) they are “La Ruda”, “Caidero de la Ruda” and “Barranquillo de la Ruda”. However, no wild populations of Ruta species have been observed so far in these areas. Although it is highly likely that these place names allude to or are related to the species described here, it is not possible to prove this. Otherwise, in the area of the Barranco de Guayadeque there are no references to wild Ruta or related toponyms.

Etymology — The specific epithet refers to the scientific entity of El Museo Canario, the institution where the mummies, branch bundle and type specimens are deposited.

Potential habitat and probable ecology

The locations of different Ruta -related place names are to the NE, SE and S of Acusa ( Fig. 6 ) and sit on rocky outcrops whose origin dates back to the Middle Miocene. According to radiometric dating by McDougall & Schmincke (1976), these formations date from 13.4 million years ago (mya). From a geological perspective, the rocks of these areas are predominantly plutonic (alkaline and peralkaline syenites) and ignimbritic tuffs, as well as peralkaline rhyolitic-trachytic lavas of the trachyrhyolitic formation of the Intracaldera Domain, Cycle I, of the volcanological history of Gran Canaria (Balcells & Barrera 1990; Balcells & al. 1990a, 1990b). The current enclave names offering clues as to the presence of Ruta are located in the C and lower area of the “cone-sheet” ( Schmincke 1967; Hernán 1976), a geological structure that clearly defines the geomorphology of the entire area.

Since their formation, the areas of Artenara and Acusa have suffered great devastation by powerful erosive processes and consequent depressions later filled with basalt, tephrite and breccias from the Roque Nublo cycle, during the Pliocene (from 5 to 3 mya). Subsequent erosion produced the current fluvial network. Such is the history of the older half of Gran Canaria, called “Palaeocanaria”, and which the volcanologists Bourcart & Jérémine (1937) defined as “Tamaran”. The subsequent history of the area is marked by a very prolonged erosive phase that modelled the landscapes into mainly rocky outcrops and imposing escarpments (lithosol-like soils) ( Balcells & al. 1990a, 1990b).

The Guayadeque area, on the contrary, is a spectacular ravine marked by almost vertical walls, with level differences of up to 300 or 400 m. The ravine was carved in lava flows (basanitic-nephelinitic lavas) and interspersed with basic pyroclasts (tephra cones and pyroclasts). These geological formations belong to the lower or middle cycle of the Post-Roque Nublo Cycle dating to the Upper Pliocene and the Lower and Middle Pleistocene (2.8 and 1.62 mya) ( Lietz & Schmincke 1975; McDougall & Schmincke 1976). The funerary sites of the area are found in outcrops of tephra cones and tuffs or pyroclasts, which are more suited to the installation of granaries as well as domestic and funerary caves.

From a biotic point of view, these processes (trachirhyolite salic formations, development of the cone sheet and the later Roque Nublo stratovolcano episode and its dismantling) must have played the role of intense evolutionary stressors for the plants of Gran Canaria, as well as being behind significant episodes of extinction ( Marrero-Rodríguez & Francisco-Ortega 2001; Marrero-Rodríguez 2004). As a consequence of this, the current vegetation, to which Ruta museocanariensis would presumably belong, is an amalgam of plant communities with a high richness of endemic species, many of them endangered, and with a fragmented distribution, as can be currently observed.

In addition to the aforementioned reasons that justify its potential distribution area (i.e. site location and the association of toponymy and geology), it is also possible to characterize the potential habitat of Ruta museocanariensis based the other plant remains in the mummy shrouds or even from the intrinsic morphological characteristics of the new species, such as being woody, more or less compact bushes with pinnatisect leaves and filiform, somewhat fleshy pinnae typical of species from a xerothermophilic environment, which broadly coincides with the surroundings of the funerary sites where they were discovered (del Arco & al. 2002).

In both analysed mummy shrouds, Ruta museocanariensis is accompanied by other species well known from these environments, such as Lavandula minutolii Bolle , Micromeria tenuis Benth. and the more or less frequent needles of Pinus canariensis . This suggests that the new Ruta could have also been collected near the indigenous settlements, at least in the Acusa area. The gathering of plants in the vicinity of the necropolis has also been verified for the site of Arteara, in the ravine of Fataga ( Jorge-Blanco 1989), where among the identified species was R. oreojasme , which grows on nearby cliffs.

Both Acusa and the Barranco de Guayadeque fall within the range of the thermomediterranean thermotype and between lower-dry and upper-semi-arid ombrotype, characterized by the presence of plant communities of the climatophilous series of Pistacio lentisci-Oleo cerasiformis sigmetum, which includes woody stands of thermophilous Juniperus canariensis , Olea cerasiformis Rivas Mart. & del Arco ( O. europaea subsp. guanchica P. Vargas & al.) and Pistacia atlantica Desf. or P. lentiscus L. ( Rivas-Martínez & al. 1993, 2002; del Arco & al. 2002). In the area of Acusa and its surroundings are thermo-sclerophyllous communities below pine forests, i.e. woodland of J. canariensis and P. atlantica , in which are found species such as Allagopappus viscosissimus Bolle , Chrysojasminum odoratissimum ( L.) Banfi ( Jasminum odoratissimum L.), Chrysoprenanthes pendula (Sch. Bip.) Bramwell ( Sonchus pendulus (Sch. Bip.) Sennikov ), Dendriopoterium pulidoi Svent. , Dracaena tamaranae Marrero Rodr. & al., Marcetella moquiniana (Webb & Berthel.) Svent. , Parolinia filifolia G. Kunkel and Teline rosmarinifolia Webb & Berthel. ( Cytisus rosmarinifolius (Webb & Berthel.) Masf. ). The surroundings of Guayadeque are made up of woodland known as “acebuchal”, in which the dominant element is Olea cerasiformis , along the lower edges of pine forests and on the edges of the E end of Monteverde. Other plants that grow in such communities include Malva acerifolia (Cav.) Alef. ( Lavatera acerifolia Cav. ), Marcetella moquiniana and Parolinia platypetala G. Kunkel.

State of the population

According to IUCN criteria and sub-criteria for assessing threatened flora ( IUCN 2012), Ruta museocanariensis is today extinct ( EX). The criterion of “extinct in the wild” ( EW) is not applicable in this case because the species is not known in cultivation or as naturalized outside its natural habitats. However, the IUCN definition of an extinct species is very tight: “ A taxon is presumed Extinct when exhaustive surveys in known and/ or expected habitat, at appropriate times (diurnal, seasonal, annual), throughout its historic range have failed to record an individual.”

The only evidence of the existence of Ruta museocanariensis consists of the material remains (branches, leaves and capsules) preserved in the mummy shrouds. It is possible to infer tentatively its previous distribution based on the location of the archaeological sites and, above all, on the survival of toponyms likely alluding to this plant. Those place-names including the word “ruda” lead to the speculation that the species did not disappear due to its use in indigenous traditions, and that it survived until after the Spanish conquest of the Canary Islands. This is based on the fact that the word “ruda” is not indigenous (Amazigh/Berber or related language), but the most widespread name bestowed on Ruta in mainland Spain (Font Quer 1990; San Miguel 2015). With the disappearance of R. museocanariensis from its natural habitat, we may be witnessing one of the consequences, poorly documented and not taken into account, of the acculturation processes carried out by the Castilian conquerors.

Phylogenetic trends in the genus Ruta

All Ruta species restricted to the Canary Islands bear 1-pinnatisect leaves, i.e. with simple pinnae, whereas their continental counterparts have 2- or 3-pinnatisect leaves ( Fig. 2 ). This falls in line with the results in previous molecular analyses mainly based on plastid DNA markers that supported the monophyly of all Canarian endemic Ruta . According to previous studies by Salvo & al. (2008, 2010) to reconstruct the phylogenetic relationships of Ruteae at a broader taxonomic and geographical level, the mainland R. montana was the closest congener of the Canarian taxa. Ruta montana is, in fact, the species whose distribution approaches the closest to the Canary Islands, as it grows throughout NW Africa down to the Anti-Atlas, where it is part of plant communities of the Macaronesian-African enclave. The molecular analyses carried out so far have failed to resolve whether the islands were colonized by a single ancestral species (unsampled species probably extinct) related to R. montana or if there were at least two colonization events ( Soto & al. 2022): one that gave rise to R. oreojasme , a species clearly differentiated both molecularly and morphologically from the others, and a second one that gave rise to the rest of the extant endemic Ruta of the islands. Ruta montana is the only species that may have leaves with linear-filiform pinnules like the pinnae of R. museocanariensis , but they are 2- or 3-pinnatisect, while the morphologically closest Canarian species, R. nanocarpa , has leaves with clearly narrowly oblanceolate pinnae, so an evolutionary sequence can be hypothesized at the morphological level, from leaves with narrow pinnae to those of increasingly broader pinnae in the W Canary Island Ruta species.

Furthermore, all continental-Mediterranean Ruta as well as R. museocanariensis and R. oreojasme on Gran Canaria have deeply lobed carpels that are connate at their base, which allows the dehiscence of the fruit. This differs from the Ruta species of the W Canary Islands, which have indehiscent fruits. In general, a deep incision of the lobes between the carpels does not always imply the dehiscence of the fruits, but in the genus Ruta it is correlated, as in all continental-Mediterranean species and in the two species of Gran Canaria. The fact that Ruta museocanariensis and R. oreojasme of Gran Canaria bear dehiscent fruits allows us to hypothesize that the evolution toward indehiscent forms has been a post-colonizing process on the islands, and marks a change from a mainly autochorous dispersal mechanism (although seeds from fruits in capsules can also be dispersed by zoochory), to one that is probably endozoochorous ( Fig. 3 ).

This putative evolutionary process from dehiscent to indehiscent fruits may also have important repercussions in the evolution of the island biota in terms of plantanimal mutualism, as has been documented in Canarian plant species such as Cneorum pulverulentum Vent. ( Neochamaelea pulverulenta (Vent.) Erdtm. ) ( Rutaceae or Cneoraceae ), Plocama pendula Aiton and Rubia fruticosa Aiton (both Rubiaceae ), and their relationship with birds or lizards ( Valido & Nogales 1994; Nogales & al. 1999; Olesen & Valido 2003, 2004; Valido & al. 2003; Traveset & Santamaría 2004).

The species from La Gomera, Ruta nanocarpa , links the two groups: although its ovaries have carpels that are lobed up to almost half of the fruit, the final dehiscence does not occur and the mature fruits remain closed ( Mesa-Coello & al. 2023). Therefore, the probable evolution from dehiscent to indehiscent fruits, as well as the shape of the leaf, suggests a stepping-stone model of inter-island colonization, which fits the age of the islands and to some extent with their distances from the mainland.

This contradicts the suggestions of Salvo & al. (2010) as to the reconstruction of ancestral areas suggesting that La Gomera was the first island to be colonized from Africa. Soto & al. (2022) based their molecular analyses on a much more extensive geographical and populational sampling of Canarian Ruta than did Salvo & al. (2010), and the results point to Gran Canaria as the most likely dispersal centre for the genus Ruta in the archipelago. This hypothesis is supported both by the high diversity of the haplotypes detected for R. oreojasme when compared with the taxa of La Gomera and by Gran Canaria’s greater age (14.5 mya vs 9.4 mya) and proximity to the continent. Moreover, both the dated phylogeny and the haplotype network obtained by Soto & al. (2022) is compatible with a stepping-stone colonization model from the continent to Gran Canaria, where R. oreojasme was the oldest taxon among the endemic Ruta analysed so far with a divergence age of approximately 5.03 mya.

Another aspect that remains unresolved is whether the W Canary Islands were colonized by the same ancestor that gave rise to Ruta oreojasme or if there were multiple colonization events from two or more ancestors, followed by speciation in different ecological habitats. The inclusion of R. museocanariensis in new molecular analyses could help determine: (1) when it arrived in the archipelago; (2) whether it is more closely related to R. nanocarpa than to R. oreojasme ; and (3) if it is the ancestral or sister species to all endemic Ruta of the W Canary Islands.

From a bioclimatic perspective, Salvo & al. (2010) commented that the genus Ruta emerged in the forests of the Mediterranean area (humid) before the current Mediterranean climate arose (drier). As this process unfolded along with various geological events (temporary terrestrial connections and fragmentations, the emergence and disappearance of continental islands), Ruta populations diversified as they were selected by the new climatic conditions to the point that they are now normally associated with characteristic elements of the current Mediterranean plant communities of Pistacia , Quercus , etc. ( Bonet 1992; Salvo & al. 2010). In the Canary Islands, although all Ruta species appear to be linked to thermo-sclerophyllous communities (of Mediterranean climate), there seems to be an inverse process to that which occurred in the Tertiary Mediterranean, leading from the most xeric Ruta species (from the W and SE parts of La Gomera, W and SW slopes in Tenerife and W and S slopes in Gran Canaria) to the most leafy Ruta species (from the E part of La Palma, N and NE slopes of La Gomera and N and E slopes of Tenerife), where Ruta is found in a diversity of plant communities, from the most xeric thermo-sclerophyllous formations to the wetter edges of the laurel forest, known as “monteverde”. The species of Gran Canaria are those that grow in more xeric environments, and although Ruta oreojasme has leaves with broad pinnae, the plant is a small, woody, stumpy and strictly rupicolous shrub with glaucous leaves.

Final remarks

The Canary Islands constitute a very abrupt volcanic archipelago marked by an extremely complex orography ( Marrero-Rodríguez & Francisco-Ortega 2001). Experiences in the field of botany serve as warnings that biodiversity lists and inventories are far from complete. New field surveys, whether casual or increasingly exhaustive, still offer remarkable surprises including, for example, the discoveries of Argyrolobium armindae Marrero Rodr. , Helianthemum inaguae Marrero Rodr. & al. and Sideritis amagroi Marrero Rodr. & B. Navarro in Gran Canaria; Helianthemum bramwelliorum Marrero Rodr. in Lanzarote; Limonium relicticum R. Mesa & A. Santos in La Gomera; and Lolium saxatile H. Scholz & S. Scholz and Ononis catalinae Reyes-Bet. & S. Scholz in Lanzarote and Fuerteventura; ( Marrero-Rodríguez 1992; Marrero-Rodríguez & al. 1995; Mesa-Coello & Santos 2001; Marrero-Rodríguez & Navarro 2003; Scholz & Scholz 2005; Marrero-Rodríguez 2008; Reyes-Betancort & Scholz 2008).

Ruta museocanariensis is possibly extinct in the wild. Several excursions and prospections carried out in near Acusa (Barranquillo de La Ruda and Cascada de La Ruda in El Carrizal, Tejeda, and in Cañada de La Ruda, Artenara) have so far been unsuccessful. However, we do not rule out that some isolated part of this island’s complex geography may still provide a surprise.

Figures

Fig. 2. Leaves of Ruta;A–D: species from N Africa and Mediterranean Europe; E–I: endemic species of the Canary Islands. – A: R. graveolens, Gran Canaria, Valleseco (LPA 2844); B: R. chalepensis, El Hierro, San Andrés (LPA 40201); C: R. angustifolia, Castellón, Benicarló (LPA 24834); D: R. montana, Morocco, between Tanalt and Tidli (LPA 34069); E: R. oreojasme, Gran Canaria, San Bartolomé de Tirajana,Amurga, Barranco del Cañizo (Arteara) (LPA 29007); F: R. museocanariensis, Gran Canaria, El Museo Canario (LPA 39792); G: R. nanocarpa, La Gomera, Roque Sombrero (LPA 34071); H: R. microcarpa, La Gomera, Alojera (LPA 36031); I: R. pinnata, Tenerife, El Tanque (LPA 34695). – Scale bar applies to A–I. – Photographs by Á. Marrero.

Fig. 3. A1–I1: fruits of Ruta species from the Canary Islands, N Africa and Mediterranean Europe. – A1: R. graveolens; B1: R. chalepensis; C1: R. angustifolia; D1: R. montana; E1: R. oreojasme; F1: R. museocanariensis; G1: R. nanocarpa; H1: R. microcarpa; I1: R. pinnata. – A2–I2: fruit primordia of same species and in same order as A1–I1, illustrating how degree of carpel fusing varies from dehiscent fruits (A2–F2), to non-dehiscent, fleshy fruits (G2–I2). – Drawn by Á. Marrero.

Fig. 5. Ruta museocanariensis – A: habit, terminal branch; B: pinna on one side of rachis, adaxial view; C: pedicel, calyx and ovary; D: terminal infructescence twig with open, star-shaped fruit, persistent calyces, bracteoles and immature fruit still closed; E: mature capsules showing dehiscence of valves, basal (left) and apical (right) views. – Image reconstructed from various twigs and fruits in different maturation stages extracted from infant mummy EMC no. 49991 (holotype and isotype) and branches and leaves extracted from adult mummy EMC no. 10 (paratypes). – Drawn by Á. Marrero.

Fig. 1. A: infant mummy EMC no. 49991, from which holotype and isotype specimens of Ruta museocanariensis were extracted; B: adult mummy EMC no. 10, from the shroud (wrapped in layers of leather) of which the two paratype specimens were extracted; C: branch bundle EMC no. 46558. – Photographs by T. Delgado, El Museo Canario.

Fig. 4A. Holotype of Ruta museocanariensis (LPA 39791). – Material extracted from infant mummy EMC no. 49991, originating, according to available data, from sepulchral archaeological sites of Acusa (Gran Canaria,Artenara), dated to 1290 BP ± 30. Leaves reveal markedly erect arrangement, maintaining rigidity of original shroud.

Fig. 6. Map of Gran Canaria indicating places (green triangles) with names that contain the word ruda (meaning Ruta) in the surroundings of Mesa de Acusa; and the spread of R. oreo- jasme (orange dots) according to BIOTA (2022). Funerary sites mentioned are indicated in the text box. – UTM coordinates, 1 × 1 km grid, GRAFCAN cartographic base, https://visor. grafcan.es/visorweb/, January 2022.