Erioscyphella tengyueica L. Luo, K. D. Hyde & C. J. Y. Li, 2025

Luo, Le, Thilini Chethana, Kandawatte W., Zhao, Qi, Su, Hong-Li, Li, Cui-Jin-Yi, Thiyagaraja, Vinodhini, Al-Otibi, Fatimah & Hyde, Kevin D., 2025, Four new species of Erioscyphella (Leotiomycetes, Helotiales) from southwestern China, MycoKeys 114, pp. 29-48 : 29-48

Erioscyphella tengyueica L. Luo, K. D. Hyde & C. J. Y. Li sp. nov.

Fig. 5

Etymology.

The epithet refers to the collection site of the type specimen.

Holotype.

HKAS 135688 .

Description.

Saprobic on the dead twigs. Sexual morph: Apothecia superficial, scattered to partly gregarious, 0.16–0.68 mm in diameter, 0.3–0.7 mm high when dry, discoid to cupulate, shortly stipitate, externally covered with short, white hairs. Discs concave, surface slightly rough, white. Margin slightly involute, white, covered with white hairs. Receptacle cupulate, concolorous, clothed entirely with short, white hairs. Stipe 0.09–0.23 mm in diameter, 0.18–0.4 mm long when dry, cylindrical, solitary, concolorous with the receptacle, clothed with white hairs. Hairs 45–95 × 2.8–6.9 µm (x ̄ = 68 × 4.8 µm, n = 10), clavate to cylindrical, straight or curved, septate, hyaline, thin-walled, less covered with hyaline granules, obtuse apex. Hymenium 65–115 µm (x ̄ = 87 µm, n = 12), concave, surface slightly rough, light white in dry. Medullary excipulum 18–33 µm (x ̄ = 26 µm, n = 18), thick, comprising hyaline, thin-walled, poorly developed cells of textura globulosa, 1.4–3.5 µm (x ̄ = 2.4 µm, n = 50) in diameter. Ectal excipulum 11–30 µm (x ̄ = 21 µm, n = 18) thick, comprising thick-walled, smooth, light yellowish cells of textura oblita to textura porrecta, 1.2–3.8 µm (x ̄ = 2.2 µm, n = 60). Paraphyses 65–97 × 1.8–3.0 µm (x ̄ = 86 × 2.5 µm, n = 25), longer than asci, filiform, straight, aseptate, hyaline, thin-walled, narrow lanceolate, smooth, less covered with hyaline granules, with slightly obtuse apex. Asci 60–80 × 6.0–9.3 µm (x ̄ = 70 × 7.6 µm, n = 34), 8 - spored, clavate, straight to slightly curved, inoperculate, hyaline, unitunicate, slightly smooth, with an apical, amyloid pore and rounded ends, croziers absent at the basal septum, J + in MLZ. Ascospores (80 / 6 / 2) 25–31.5 × 1.6–5.5 µm, (x ̄ = 27.7 × 3.3 µm, n = 80), overlapping biseriate, filiform, aseptate, thin-walled, hyaline, rough with tapering towards obtuse ends, filled with oil guttules or with 1–2 - large oil guttules. Asexual morph: Not observed.

Material examined.

China • Yunnan Province, Tengchong City, Tengyue Street , altitude 1983.3 m, on the decayed unidentified twig, 21 August 2022, Cuijinyi Li, LCJY 1171 ( HKAS 135688 , holotype) ; • ibid., altitude 1774 m, on the decayed unidentified twig, 18 August 2022, Le Luo, Ly 255 ( HKAS 135693 , paratype) .

Notes.

Our specimens, HKAS 135688 and HKAS 135693 , were grouped into a distinct clade, separated from E. papillaris ( TNS-F-81272 ) by 91 % MLBS and 0.98 BIPP (Fig. 1 ). Erioscyphella tengyueica differs from E. papillaris by having aseptate ascospores and aseptate paraphyses, while the latter has septate paraphyses and aseptate or one-septate (rarely two-septate) ascospores. Our species differs from E. otanii by having longer asci (60–80 µm vs. 34–38.8 µm), longer ascospores (34–38.8 µm vs. 12.3–14.6 µm) and aseptate paraphyses, in contrast to the septate paraphyses of E. otanii ( Tochihara and Hosoya 2022) . Therefore, E. tengyueica is introduced here as a new species.

Figures

Figure 1. The Maximum Likelihood tree based on the combined LSU, ITS, mtSSU and RPB 2 sequence data for Erioscyphella. Capitotricha bicolor (TNS-F- 65670), C. rubi (TNS-F- 65752), Lachnellula calyciformis (TNS-F- 81248), L. suecica (TNS-F- 16529) and Neodasyscypha cerina (TNS-F- 65625) are used as the outgroup taxa. The MLBP ≥ 70 % and BPP ≥ 0.90 are shown at the nodes as MLBP / BPP. MLBS < 70 % and BPP < 0.90 are expressed as a hyphen (“ - ”). Names with (T) indicate type specimens. Names in red indicate new species.

Figure 5. Erioscyphella tengyueica (HKAS 135688, holotype) a – c dried ascomata on the host d a vertical section of an ascoma e excipulum f paraphyses g – j asci (i, j asci in MLZ) k ascospores. Scale bars: 100 µm (d); 50 µm (e, f); 20 µm (g – j); 10 µm (k) .