Papiliotrema mussaendae C. Y. Chai & F. L. Hui, 2025

Gao, Wan-Li, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Two new species of Papiliotrema (Rhynchogastremataceae, Tremellales) from China, MycoKeys 126, pp. 171-186 : 171-186

Papiliotrema mussaendae C. Y. Chai & F. L. Hui sp. nov.

Fig. 3

Etymology.

The specific epithet mussaendae refers to Mussaenda, the plant genus from which the type strain was isolated.

Typus.

China • Guizhou, Pingtang County, Sifangjing Village , 25°7'N, 107°2'E, on the phylloplane of Mussaenda pubescens , Feb 2023, D. Lu, NYNU 23248 ( holotype CICC 33595 , preserved in a metabolically inactive state, metabolically inactive ex-type culture PYCC 9975 ), GenBank Accession No.: OQ 851892 ( ITS), OQ 851890 ( LSU) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is white-cream, mucoid, smooth and shiny, with an entire margin. After 3 days in YM broth at 20 ° C, cells are ovoid and ellipsoidal, 3.1–4.7 × 3.4–5.7 μm and single, budding is polar. After 1 month at 20 ° C, a ring and a sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol (weak), glycerol (delayed), erythritol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, citrate, D-gluconate, D-glucosamine, N-acetyl-D-glucosamine (delayed), 2 - keto-D-gluconate, D-glucuronate and glucono- 1, 5 - lactone are assimilated as carbon sources. Methanol is not assimilated. Nitrate, nitrite, ethylamine, L-lysine and cadaverine are assimilated as nitrogen sources. Growth is observed at 20 ° C and 25 ° C, but not at 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is positive. Vitamins are not necessary for growth. Starch-like compounds are produced. Diazonium blue B colour and urease reaction are positive.

Additional strain examined.

China, Guizhou, Pingtang County, Sifangjing Village , 25°7'N, 107°2'E, on the phylloplane of Mussaenda pubescens , Feb 2023, D. Lu, NYNU 232142 and NYNU 23229 , GenBank Accession No.: PV 823294 and PV 823295 ( ITS), PV 823293 and PV 823292 ( LSU) .

Note.

Phylogenetic analyses indicate P. mussaendae is most closely related to P. wisconsinensis . Both differ in their ability to assimilate L-sorbose and nitrate and its ability to grow at 30 ° C from P. wisconsinensis (Table 2). Morphologically differs from P. wisconsinensis by forming white-cream colonies and smaller cells. Morphologically differs from P. wisconsinensis by forming white-cream colonies and smaller cells.

Figures

Figure 3. Morphology of Papiliotrema mussaendae (NYNU 23248). A. Colony on YM agar after 3 days at 20 ° C; B. Budding cells in YM broth after 3 days at 20 ° C.