Dasyspora nitidae Beenken

Gomes, Gardênia Figueiredo, Sotão, Helen Maria Pontes, Dealmeida, Layse Barreto, França, Isadora Fernandes De & Monteiro, Josiane Santana, 2025, Dasyspora (Pucciniales) on Xylopia in the Amazon, with new distribution records for Brazil, Phytotaxa 697 (1), pp. 68-82 : 77-78

Dasyspora nitidae Beenken , in Beenken, Zoller & Berndt, Mycologia 104(3): 675 (2012). Figs. 7 , 9B

Holotype: FRENCH GUIANA: on X. nitida Dunal , 28 July 2009, Roura canton, road to Kaw (D6), side road to Fougasier, Montagne de Kaw, 4u309 N, 52u109 W, 100 m, L. Beenken & R. Berndt No. 28.07.09/6 (in PC). Mycobank number: 519389.

Spermogonia subepidermal, group VI, type 5. Aecia and uredinia unknown. Telia abaxial on leaves, erumpent, dark brown, solitary or aggregate in yellowish spots arranged in more or less spread circles (3–5 × 2–7 mm). Paraphyses rare, peripheral, clavate at the tips. Teliospores 2-celled, ellipsoid (28–40 × 19.5–27 µm), septum transverse, wall ornamented with areolate warts (0.5–1 µm); apical and basal rod-like projections branched at the tip (3–10 × 1–1.5 µm); pedicel fragile, hyaline, usually brittle.

Specimens examined:— BRAZIL. AMAPÁ: Mazagão, Camaipi , on X. benthamii , 15 November 1987, J. F. Hennen & Sotão 87-129 ( MG173301 ) ; PARÁ: Floresta Nacional de Caxiuanã , on X. nitida , 4 October 2003, O. Cardoso et al. 1227 ( MG171765 ). Tucuruí, January 1981, P. Lisboa et al. ( MG96443 ) .

Host and distribution in South America:— On leaves of X. nitida in French Guiana ( Beenken et al. 2012). In the present work, we present the first record of the species in Brazil (Amapá and Pará) and X. benthamii as a new host for this species.

Comments:— Dasyspora nitidae differs from other species in the clade Dasyspora winteri by the ornamentation of the teliospore wall, which is similar in the lateral region and close to the septum, in addition to the apical and basal projections that are the longest observed in the clade, reaching up to 10 µm. Dasyspora ferruginae was also found growing on X. nitida , but it differs from D. nitidae mainly in the color and arrangement of the warts on the wall of the spores, which are differentiated in the septum region and the sides of the spores. Beenken et al. (2012) also recorded D. guianensis on X. benthamii in French Guiana. Dasyspora guianensis differs from D. nitidae in its smaller teliospores (29–32 × 21.5–24 µm) and smaller apical and basal projections (up to 5 × 2 µm), while D. nitidae has larger teliospores (28–40 × 19.5–27 µm) and longer apical and basal projections (up to 10 × 1.5 µm).

Beenken et al. (2012) mentioned that in addition to the occurrence on X. nitida , there were two plant specimens remained as uncertain ( Xylopia cf. nitida ); in the type specimen of D. nitidae ( X. nitida ), white hairs were observed on the abaxial surface of the leaf, while in the uncertain specimens ( Xylopia cf. nitida ), the hairs were brown on the adaxial surface of the leaf. The specimens considered “uncertain” due to the presence of brown hairs may belong to X. benthamii , as described in the botanical taxonomic works by Ribeiro et al. (1999) and Maas et al. (2007). A taxonomic revision of the host specimens mentioned by Beenken et al. (2012) is, therefore, necessary.

Identification key for Dasyspora species on Xylopia in South America (adapted from Beenken et al. 2012)

1. Teliospore wall tuberculate to areolate with hemispherical to conical warts, warts in the septum region do not form distinct rows. ............................................................................................................................................................................................................2

- Teliospore wall polygonal, sub-polygonal to areolate with flattened warts, warts in the septum region usually form two distinct rows ....................................................................................................................................................................................................4

2. Lateral walls with hemispherical warts, with apical and basal projections measuring 3–13 ×1–2 µm .............................................3

- Lateral walls with conical (echinulate) warts, with apical and basal projections measuring 1.7–7 × 1–2 µm .... Dasyspora echinata

3. Smooth region around the septum well differentiated, forming a smooth band, 3–5 µm thick.......................... Dasyspora gregaria

- Smooth region around the septum smaller, forming a smooth band, 1–2 µm thick......................................... Dasyspora segregaria

4. Telia not forming spots, on leaves of X. emarginata ..................................................................................... Dasyspora emarginatae

- Telia in yellowish spots on other Xylopia species..............................................................................................................................5

5. Warts in the lateral wall and septum region with a similar rounded pattern, slightly sub-polygonal in the septum region, measuring 1–2 µm in the lateral wall and septum region ........................................................................................................ Dasyspora nitidae

- Warts in the lateral wall and septum region polygonal to sub-polygonal, with different dimensions, warts in the lateral wall 0.5–2 µm and warts in the septum region measuring 0.5–4 µm ........................................................................................................6

6. Ornamentation densely arranged in the septum region, eventually forming plaques ........................................................................7

- Ornamentation in the septum region more spaced and not forming plaques .....................................................................................8

7. Spore wall and ornamentations dark brown in lactoglycerol, teliospores 30–36 × 21–24 µm ....................... Dasyspora ferrugineae

- Spore wall and ornamentations light brown in lactoglycerol, teliospores 15–30 × 12–21 µm........................ Dasyspora amazonica

8. Apical and basal projections up to 5 × 2 µm, teliospores 29–32 × 21.5–24 µm................................................. asyspora guianensis

- Apical and basal projections up to 6–8 × 1–1.5 µm, teliospores 28–39 × 18–27 µm........................................................................9

9. Teliospores not constricted or slightly constricted in the septum, lateral warts usually elongated transversely (equatorially), apical and basal projections up to 8 × 1.5 µm, teliospores 28–38 × 18–25 µm......................................................... Dasyspora frutescentis

- Teliospores constricted in the septum, lateral warts generally elongated longitudinally, apical and basal projections up to 7 × 1 µm, teliospores 30–39 × 20–27 µm ............................................................................................................................... Dasyspora winteri

Figures

FIGURE 7. Dasyspora nitidae. A–B. Telia on Xylopia nitida. A. Telia in stereomicroscope; B. Telia in SEM; C. Teliospores in OM; D. Teliospores in SEM. Bars: A= 0.5 mm, B= 50 µm, C, D= 10 µm.

FIGURE 9. Teliospores from specimens of the Dasyspora winteri clade from the Amazon in OM and SEM. A) D. amazonica (MG 180446), B) D. nitidae (MG 173301), C) D. ferrugineae (MG 173304). Bars: 10µm.