Carlosrosaea camelliae W. L. Gao & F. L. Hui, 2025

Gao, Wan-Li, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Three new species of Carlosrosaea (Trimorphomycetaceae, Tremellales) from China, MycoKeys 119, pp. 123-135 : 123-135

Carlosrosaea camelliae W. L. Gao & F. L. Hui sp. nov.

Fig. 2 A, B

Etymology.

The specific epithet camelliae refers to Camellia , the name of the genus of the plant from which the type species was collected.

Type.

China • Fujian Prov.: Quanzhou City, Qingyuan Mountain , 25°7'N, 118°44'E, in the phylloplane of Camellia sp. , March 2022, W. T. Hu and S. B. Chu, NYNU 223230 (holotype CICC 33566 T, preserved in a metabolically inactive state; culture ex-type PYCC 9958 , preserved in a viable metabolically inactive state; GenBank: OP 278681 , OP 278682 ) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is white to pale-yellow, butyrous, smooth, and glossy, with an entire margin (Fig. 2 A ). After 3 days in YM broth at 20 ° C, cells are ovoid, 2.1–4.0 × 2.6–4.9 μm, and single; budding is polar (Fig. 2 B ). After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA, or V 8 agar. Ballistoconidia are not produced. Glucose fermentation is absent. The following compounds are assimilated as sole carbon sources: glucose, sucrose, raffinose, melibiose, galactose, trehalose (weak), maltose (weak), melezitose, methyl-α-D-glucoside, cellobiose (weak), salicin (delayed and weak), L-sorbose, L-rhamnose (weak), D-xylose, L-arabinose (weak), D-arabinose, 5 - keto-D-gluconate, D-ribose, glycerol (weak and delayed), erythritol, ribitol (delayed), galactitol, D-mannitol, D-glucitol, DL-lactate (delayed), succinate, citrate, D-gluconate, D-glucosamine (weak), N-acetyl-D-glucosamine, 2 - keto-D-gluconate (weak), D-glucuronate (weak), and glucono- 1, 5 - lactone. Inulin, lactose, methanol, ethanol, and myo-inositol are not assimilated. Nitrate, nitrite, ethylamine, L-lysine, and cadaverine are not assimilated as sole nitrogen sources. Maximum growth temperature is 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Fujian Prov.: Quanzhou City, Qingyuan Mountain , 25°7'N, 118°44'E, in the phylloplane of Camellia sp. , March 2022, W. T. Hu and S. B. Chu, NYNU 223212 .

Note.

C. camelliae sp. nov. is phylogenetically closely related to C. glechomae sp. nov., which is also described in this study, but they exhibit clear morphological and physiological differences (Table 2 ). Colonies of C. camelliae sp. nov. are white to pale yellow on YM agar, whereas those of C. glechomae sp. nov. are white to cream-colored. C. camelliae sp. nov. produces ovoid cells, while C. glechomae sp. nov. forms cylindrical cells. In addition, the cells of C. camelliae sp. nov. are shorter (2.6–4.9 μm) compared to those of C. glechomae sp. nov. (3.3–15 μm). Physiologically, C. camelliae sp. nov. differs from C. glechomae sp. nov. by its inability to assimilate inulin, lactose, myo-inositol, nitrate, and L-lysine, and its ability to assimilate glycerol and erythritol.

Figures

Figure 2. Carlosrosaea camelliae sp. nov. (NYNU 223230 T). A Culture on YM agar at 20 ° C after 7 d; B. Budding cells grown in YM broth at 20 ° C after 3 d. Carlosrosaea glechomae sp. nov. (NYNU 2311170 T); C. Culture on YM agar at 20 ° C after 7 d; D. Budding cells in YM broth at 20 ° C after 3 d. Carlosrosaea wuzhiensis sp. nov. (NYNU 24841 T); E. Culture on YM agar at 20 ° C after 7 d; F. Budding cells grown in YM broth at 20 ° C after 3 d. Scale bars: 10 μm.