Torulaspora cukson, Silva, M. R., Paraíso, F., Al-Oboudi, J., Abegg, M., Aires, A., Barros, K. O., Brito, P. H., Jarzyna, M., Sylvester, K., Langdon, Q. K., Opulente, D. A., Carriconde, F., Fell, J. W., Hofmann, T. A., Lachance, M. - A., Legras, J. - L., Libkind, D., Pontes, A., Gonçalves, P., Rosa, C. A., Groenewald, M., Hittinger, C. T. & Sampaio, J. P., 2025

Silva, M. R., Paraíso, F., Al-Oboudi, J., Abegg, M., Aires, A., Barros, K. O., Brito, P. H., Jarzyna, M., Sylvester, K., Langdon, Q. K., Opulente, D. A., Carriconde, F., Fell, J. W., Hofmann, T. A., Lachance, M. - A., Legras, J. - L., Libkind, D., Pontes, A., Gonçalves, P., Rosa, C. A., Groenewald, M., Hittinger, C. T. & Sampaio, J. P., 2025, A taxogenomic view of the genus Torulaspora: an expansion from ten to twenty-two species, Persoonia 54 (1), pp. 265-283 : 275

Torulaspora cukson f.a. M. Silva, F. Paraíso, M.-A. Lachance & J.P. Sampaio, sp. nov. MB 853718 . Fig. 4D .

Etymology: Torulaspora cukson . cuk’son, N.L. app. n. cukson, from Cuk Son, the designation in Uto-Aztecan Oʼodham language for Tucson, the locality in which this species was originally found.

Typus: USA, Arizona, Scaptodrosophila brooksae on Populus fremontii , 1985, M.A. Lachance (holotype PYCC 9174H, ex-holotype cultures PYCC 9174, CBS 18510). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ/ENA/ GenBank under the accession GCA_964263435. The version described in this paper is v. 1.

Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are ellipsoidal (3–5 × 2–3 µm) and occur singly or in pairs, and proliferation is by multilateral budding ( Fig. 4D ). On Dalmau plates after 2 wk at 25 °C, pseudohyphae and true hyphae are not formed. Sexual reproduction was not observed on acetate agar or corn meal agar after prolonged incubation (3 mo) at 18 °C. The physiological and biochemical profile of the species are shown in Table 1 and Table S5.

Habitat and distribution: This species was found only once, in an insect ( Scaptodrosophila brooksae ), Arisona, USA.

Figures

Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.