Torulaspora mapucheana M. Silva, F. Paraíso, D. Libkind & J.P. Sampaio, 2025

Silva, M. R., Paraíso, F., Al-Oboudi, J., Abegg, M., Aires, A., Barros, K. O., Brito, P. H., Jarzyna, M., Sylvester, K., Langdon, Q. K., Opulente, D. A., Carriconde, F., Fell, J. W., Hofmann, T. A., Lachance, M. - A., Legras, J. - L., Libkind, D., Pontes, A., Gonçalves, P., Rosa, C. A., Groenewald, M., Hittinger, C. T. & Sampaio, J. P., 2025, A taxogenomic view of the genus Torulaspora: an expansion from ten to twenty-two species, Persoonia 54 (1), pp. 265-283 : 276-277

Torulaspora mapucheana M. Silva, F. Paraíso, D. Libkind & J.P. Sampaio , sp. nov. MB 853980 . Fig. 4P–R .

Etymology: Torulaspora mapucheana . ma.pu’che.a.na, N.L. fem. adj. mapucheana, referring to Mapuche, the group of indigenous inhabitants of present-day south-central Chile and southwestern Argentina.

Typus: Argentina, Lanin National Park , Yuco, bark of Nothofagus obliqua , 2009, D. Libkind (holotype PYCC 9893 H, ex-holotype cultures PYCC 9893 , CBS 18512 ). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/GenBank under the accession GCA_964263445. The version described in this paper is v. 1 .

Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are globose (1.5–4 µm) to sub-globose (4–5 × 2–3 µm) and occur singly or in pairs, and proliferation is by multilateral budding ( Fig. 4P ). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction is observed on acetate agar after 15 d at 20 °C and the cultures appear to be homothallic. Cellular extensions that resemble conjugation tubes are frequent and usually are not involved in conjugation ( Fig. 4.Q ). Asci are persistent and normally are formed after conjugation involving a cell and its bud. Asci produce one to two smooth and spherical ascospores, measuring 2–3 µm diam. ( Fig. 4R ). The physiological and biochemical profile of the species in shown in Table 1 and Table S5.

Habitat and distribution: This species was found in the arboreal niche, in association with Nothofagus spp. Cultures were isolated from the bark of Nothofagus obliqua and the fruiting body of Cyttaria harioti on N. antarctica . Currently known from collections in Patagonia, Argentina (Yuco and Los Rápidos, Nahuel Huapi natural park).

Additional cultures examined: PYCC 9894, PYCC 9057 (see Table S1 for details).

Figures

Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.