Phyllozyma camelliae Z. W. Xi & F. L. Hui, 2025

Xi, Zhi-Wen, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Phylogeny of Phyllozyma (Spiculogloeaceae, Spiculogloeales) with description of four new species from China, MycoKeys 123, pp. 235-251 : 235-251

Phyllozyma camelliae Z. W. Xi & F. L. Hui sp. nov.

Fig. 4

Etymology.

The specific epithet “ camelliae ” refers to Camellia, the plant genus from which the type strain was isolated.

Typus.

China • Hainan Prov.: Qiongzhong Li and Miao Autonomous County, Diaoluo Mountain , in the phylloplane of Camellia oleifera , 15 Jul 2023, X. M. Han, NYNU 23731 ( holotype CICC 33625 T preserved as a metabolically inactive state, culture ex-type PYCC 9991 ) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is pale-yellow, mucoid, smooth, and glistening, with an entire margin. After 3 days in YM broth at 20 ° C, cells are cylindrical, 1.5–2.4 × 5.3–8.5 μm and single, budding is polar. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA, or V 8 agar. Ballistoconidia are ellipsoidal, 2.0–2.7 × 2.7–4.3 μm. Glucose fermentation is absent. Glucose, inulin (delayed and weak), galactose (delayed), trehalose, D-arabinose (delayed), glycerol (delayed), D-mannitol, D-glucitol (delayed), DL-lactate (delayed and weak), and succinate (delayed and weak) are assimilated as sole carbon sources. Sucrose, raffinose, melibiose, lactose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, 5 - keto-D-gluconate, D-ribose, methanol, ethanol, erythritol, ribitol, galactitol, myo-inositol, citrate, D-gluconate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone are not assimilated. Nitrate, nitrite (delayed), ethylamine (delayed), and L-lysine are assimilated as sole nitrogen sources. Cadaverine is not assimilated. Maximum growth temperature is 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Hainan Prov.: Wuzhishan City, Wuzhi Mountain , in the phylloplane of Symplocos adenophylla , 12 Aug 2024, Y. F. Lu & F. L. Hui, NYNU 24899 .

GenBank accession numbers.

Holotype CICC 33625 T ( ITS: PP 033661, D 1 / D 2: PP 033657, RPB 1: PX 353019, TEF 1: PV 654542); additional strain NYNU 24899 ( ITS: PP 239073, D 1 / D 2: PP 239062, RPB 1: PX 353020, TEF 1: PV 654543).

Note.

See the “ Notes ” of the previous species.

Figures

Figure 4. Morphological characteristics of P. camelliae sp. nov. NYNU 23731 T. A. The streak culture grown on YM agar after 7 d at 20 ° C; B. Budding cells grown in YM broth for 3 d at 20 ° C; C. Ballistoconidia produced on CMA after 7 d at 20 ° C. Scale bars: 10 μm.