Dna extraction

Asgari, Mohsen, Mokhtari, Somayeh, Eskandari, Ali & Peña-Santiago, Reyes, 2025, Description and molecular characterisation of Aquatides caelibis sp. n. (Dorylaimida: Nygolaimidae) from Iran, with updated taxonomy of the genus and new insights into its evolutionary relationships, Journal of Natural History 59 (25 - 28), pp. 1877-1893 : 1878

Sampling, extraction and morphological identification of nematodes

Several moss samples were collected from a few habitats and localities of Siahkal forests, located in Gilan province, northern Iran. The samples were cut into smaller pieces for nematode extraction. Nematodes were extracted using the tray method (Whitehead and Hemming 1965), handpicked under a stereomicroscope, killed by adding hot FPG (4:1:1 formaldehyde: propionic acid:glycerine) solution, transferred to anhydrous glycerine according to De Grisse (1969), and mounted on permanent glass slides to be observed under light microscopy.

Specimens were examined using a Nikon Eclipse 80i light microscope provided with differential interference contrast ( DIC) optics, a Nikon Digital Sight DS-U1 camera and a drawing tube . Raw photographs were edited using Adobe ® Photoshop ® CS . Morphometrics include Demanian indices and other measurements and ratios; the most relevant of them are presented in a separate table, while others appear to form part of the literal description of the species. The female holotype and paratypes are deposited in the Nematode Collections of Universidad de Jaén, Spain, and University of Zanjan, Iran . Please refer to the ‘Type material’ section for more details.