Phyllozyma aucubae Z. W. Xi & F. L. Hui, 2025

Xi, Zhi-Wen, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Phylogeny of Phyllozyma (Spiculogloeaceae, Spiculogloeales) with description of four new species from China, MycoKeys 123, pp. 235-251 : 235-251

Phyllozyma aucubae Z. W. Xi & F. L. Hui sp. nov.

Fig. 3

Etymology.

The specific epithet “ aucubae ” refers to Aucuba, the plant genus from which the type strain was isolated.

Typus.

China • Guizhou Prov.: Guiyang City, East Mountain Park , in the phylloplane of Aucuba japonica , 15 Sept 2023, D. Lu, NYNU 239180 ( holotype CICC 33627 T preserved as a metabolically inactive state, culture ex-type PYCC 9993 ) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is cream, mucoid, smooth, and glistening, with an entire margin. After 3 days in YM broth at 20 ° C, cells are cylindrical, 2.1–2.2 × 6.4–11.9 μm and single, budding is polar. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, simple pseudohyphae and formed. Sexual structures are not observed on PDA, CMA, or V 8 agar. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, inulin (weak), D-arabinose (delayed), glycerol (delayed), D-mannitol, D-glucitol (delayed), DL-lactate (delayed and weak), and succinate (delayed) are assimilated as sole carbon sources. Sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, 5 - keto-D-gluconate, D-ribose, methanol, ethanol, erythritol, ribitol, galactitol, myo-inositol, citrate, D-gluconate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone are not assimilated. Nitrate (delayed and weak), nitrite (delayed and weak), ethylamine (delayed), and L-lysine are assimilated as sole nitrogen sources. Cadaverine is not assimilated. Maximum growth temperature is 25 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Guizhou Prov.: Guiyang City, East Mountain Park , in the phylloplane of Aucuba japonica , 15 Sept 2023, D. Lu, NYNU 239198 .

GenBank accession numbers.

Holotype CICC 33627 T ( ITS: OR 961460, D 1 / D 2: OR 958754, RPB 1: PX 353021, TEF 1: PV 654547); additional strain NYNU 239198 ( ITS: PP 660918, D 1 / D 2: PP 660917, TEF 1: PV 654548).

Note.

Physiologically, P. aucubae sp. nov. differs from its closely related species, P. camelliae sp. nov., described in this study, by its inability to assimilate galactose and trehalose, as well as its ability to grow at 30 ° C (Table 2 ).

Figures

Figure 3. Morphological characteristics of P. aucubae sp. nov. NYNU 239180 T. A. The streak culture grown on YM agar after 7 d at 20 ° C; B. Budding cells grown in YM broth for 3 d at 20 ° C; C. Simple pseudohyphae produced on CMA after 7 d at 20 ° C. Scale bars: 10 μm.