Vishniacozyma pingtangensis C. Y. Chai & F. L. Hui, 2025

Liu, Shan, Cai, Dan-Yang, Chai, Chun-Yue & Hui, Feng-Li, 2025, Five new epiphytic species of Vishniacozyma (Bulleribasidiaceae, Tremellales) from China, MycoKeys 113, pp. 321-336 : 321-336

Vishniacozyma pingtangensis C. Y. Chai & F. L. Hui sp. nov.

Fig. 2 C

Etymology.

The specific epithet pingtangensis refers to the geographic origin of the type strain, Pingtang County, Guizhou Province.

Typus.

China. • Guizhou Prov.: Pingtang Co., Sifangjing Vil. (25 ° 7 ' 45 ' N, 107°2'54"E), Feb 2023, in the phylloplane of Acer saccharum , D. Lu, NYNU 23281 (holotype CICC 33596 T, GenBank: OQ 851896 , OQ 851894 ); culture ex-type PYCC 9976 .

Description.

On YM agar after seven days at 20 ° C, the streak culture is white-cream, mucoid, glistening, and smooth, with an entire margin. After three days in YM broth at 20 ° C, cells are ellipsoida, 2.4–3.7 × 3.7–6.4 μm, and single; budding is polar. After one month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. On corn meal agar, ballistoconidia are not produced. Glucose fermentation is absent. Glucose, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose (weak), L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol (weak), glycerol (delayed and weak), ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate (delayed), succinate (weak), D-gluconate, D-glucosamine (weak), N-acetyl-D-glucosamine, 2 - keto-D-gluconate, D-glucuronate (weak), and glucono- 1, 5 - lactone are assimilated as sole carbon sources. Inulin, methanol, and erythritol are not assimilated. Nitrate, nitrite (weak), ethylamine, L-lysine (weak), and cadaverine (delayed) are assimilated as sole nitrogen sources. Maximum growth temperature is 25 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China. • Henan Prov.: Songxian Co., Tianchi mountain (34°32'27"N, 112°16'39"E), Jun 2023, in the phylloplane of Morus alba , J. Z. Li, NYNU 236258 (GenBank: PQ 496708 , PQ 496707 ) .

Note.

In the phylogenetic analyses, V. pingtangensis was closely related to V. eriobotryae ; both differ by 102 nucleotides (52 / 487 in ITS and 50 / 600 in LSU). Physiologically, they can be differentiated because V. eriobotryae has the ability to assimilate glycerol and the inability to assimilate inulin and erythritol, in addition to being able to grow at 30 ° C, while V. pingtangensis cannot.

Figures

Figure 2. Budding cells of A Vishniacozyma diospyri sp. nov. NYNU 221044 T B V. guiyangensis sp. nov. NYNU 22831 T C V. pingtangensis sp. nov. NYNU 23281 T D V. eriobotryae sp. nov. NYNU 229203 T E V. tianchiensis sp. nov. NYNU 236163 T following growth in YM broth for three days at 20 ° C F Elongate budding cells of V. diospyri sp. nov. NYNU 221044 T following growth on CMA for seven days at 20 ° C. Scale bars: 10 μm.