Anteaglonium parvulum (Schwein.) Mugambi & Huhndorf, Syst. Biodiv.

Xu, Rui-Fang, Karunarathna, Samantha C., Hyde, Kevin D., Kakumyan, Pattana, Elgorban, Abdallah M., Phukhamsakda, Chayanard & Tibpromma, Saowaluck, 2025, Morphology and multigene phylogeny reveal a new species and a new record of saprobic Dothideomycetes from Hevea brasiliensis in Yunnan Province, China, Phytotaxa 690 (1), pp. 63-76 : 66-67

Anteaglonium parvulum (Schwein.) Mugambi & Huhndorf, Syst. Biodiv. 7(4): 460 (2009) Fig. 2

Index Fungorum Number: IF543261; Facesoffungi number: FoF01931

Saprobic on dead branch of Hevea brasiliensis . Sexual morph: Ascomata 420–810 × 180–320 μm, (x = 600 × 263 µm, n = 25), superficial or semi-immersed or immersed at the base, oval to elongate or subglobose, uni to multi-loculate, carbonaceous, straight or curved, black. Peridium 30–90 μm thick, relatively thick, strongly carbonaceous texture, composed of thick-walled dark angular or relatively compressed pseudoparenchymatous cells, inner layers composed of hyaline to brown cells of textura angularis. Hamathecium comprising 1–2 μm wide, numerous, cylindrical to filiform, hyaline, branched, septate, anastomosing pseudoparaphyses. Asci 40–75 × 5–9 μm (x = 57 × 7 µm, n = 20), 8-spored, bitunicate, fissitunicate, elongate cylindric-clavate, straight or slightly curved, sessile or with short pedicel, apically rounded, with a minute ocular chamber. Ascospores 5–10 × 2–4.5 μm (x = 7 × 3 µm, n = 20), 1-seriate, hyaline, ellipsoidal, 1-septate, constricted at the septa, upper cell wider and tapering towards the narrow ends, guttulate, smooth-walled. Asexual morph: Coelomycetous. Conidiomata grouped to scattered, superficial, semi-immersed at the base, subglobose to globose, brown to dark brown. Conidioma wall pseudoparenchymatous, composed of dark brown cells of textura angularis. Conidiophores reduce to conidiogenous cell. Conidiogenous cells phialidic, hyaline, simple, and smooth, with a conspicuous collarette at the apex. Conidia 2–3.5 × 1.5–2 μm (x = 2.8 × 1.7 µm, n = 25), 1-celled, oblong to ellipsoidal or oval, rounded ends, slightly curved, aseptate, hyaline, smooth-walled.

Culture characteristics: Ascospores germinated on PDA within 24h with germ tubes produced from both end cells, circular, cottony, raised, entire margin, superficial, smooth with margin, colony from above, irregular in the center, entire edges, brown; from below, orange in the center, brown edges, with smooth margin, producing orange pigmentation in PDA, the asexual state developed within a three months period when cultured on PDA under dark conditions, maintained at temperatures ranging between 15 °C and 25 °C.

Material examined: China, Yunnan Province, Lincang , Yongde County, on a dead branch of Hevea brasiliensis , 18 March 2023, Rui-Fang Xu, PWR-03 (GMB-W1071) ; living culture, GMBCC 1089.

GenBank numbers: GMBCC 1089 = ITS: PQ724979, LSU: PQ725019, SSU: PQ725027, tef 1-α: PQ757609, rpb 2: PQ757606

Notes: Based on blast results, ITS, LSU and SSU sequences of our isolate had 99.37%, 100%, and 100% similarities to Anteaglonium parvulum (MFLUCC 10-0928) respectively; tef 1-α sequence had 99.86% similarity to Anteaglonium parvulum (C 09); rpb 2 sequence had 90.89% similarity to A. rubescens (OR1). The new isolate (GMBCC 1089) formed a group together with A. parvulum (CBS 205.34, MFLUCC 10-0928) in the combined ITS, LSU, SSU, tef 1-α, and rpb 2 phylogenetic analysis with 91% ML. As sexual and asexual morphological characteristics in ascomata, ascospore, and conidia of our strain largely overlap with A. parvulum (MFLUCC 10-0928), we report our isolate (GMBCC 1089) as a new host record of Hevea brasiliensis . Anteaglonium parvulum (MFLUCC 10-0928) has been recorded from undetermined trees in Thailand ( Jayasiri et al. 2016; Hyde et al. 2020), dead wood from the USA ( Mugambi & Huhndorf 2009), and Murraya exotica in India (Hongsanan et al. 2020). This is the first host and country record of A. parvulum from Hevea brasiliensis in Yunnan Province, China.

Figures

FIGURE 2. Anteaglonium parvulum (GMB-W1071, new host and new geographical record). a–c Habit on substrate. d Section of ascoma. e–f Asci with pseudoparaphyses. g Ascus. h–l Ascospores. m Germinated ascospore. n, o Colonies on PDA media. p, q Conidiomata on PDA. r Squash mount of conidioma. s Conidia. Scale bars: d–f = 50 μm, g = 30 μm, h–l, s = 5 μm, m = 20 μm, r = 200 μm.