Pyrenochaetopsis tabarestanensis M. Papizadeh, Soudi, Amini, Wijayaw. & K.D. Hyde, 2017

Papizadeh, Moslem, Soudi, Mohammad Reza, Amini, Leila, Wijayawardene, Nalin N. & Hyde, Kevin D., 2017, Pyrenochaetopsis tabarestanensis (Cucurbitariaceae, Pleosporales), a new species isolated from rice farms in north Iran, Phytotaxa 297 (1), pp. 15-28 : 20-23

Pyrenochaetopsis tabarestanensis M. Papizadeh, Soudi, Amini, Wijayaw. & K.D. Hyde , sp. nov. ( Fig. 1).

MycoBank: MB 805919, Facesoffungi number: FoF 01918.

Etymology:―tabarestanensis, from Tabarestan, the ancient name of the Mazandaran Province where the strain was isolated.

Type:― IRAN. Mazandaran Province: Amol, Buran Village , isolated from soil (rice farms: N: 36° 27.5 3 and E: 052° 23.8 31), 21 Feb. 2011, Leila Amini ( IBRC-H 2019, holotype), ex-type living cultures IBRC-M 30051 = CBS 139506 .

Additional strain: IBRC-M 30047; isolated as the same as IBRC-M 30051.

Pycnidia up to 350 diam. (on OA medium), solitary or arranged in clusters of up to 4, globose to subglobose and rarely flask-shaped, unilocular, ostiolate. Ostiole apapillate, single, central, and circular. Conidiomata wall of 2–3 layers of brown-walled cells of textura angularis. Setae exclusively ostiolar, approximately straight, unbranched, tapered towards the apex. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 4–5 × 3–4 μm, with a single supporting cell, subcylindrical to ampulliform, hyaline, smooth. Conidia 3.5–6 × 1.5–2.5 μm (x = 4.75 × 2 μm, n = 20), hyaline, aseptate, cylindrical to oblong and tapered to the both ends. Terminal and intercalary chlamydospores, with diameter of 8–13 μm, solitary but also in chains and rarely in clusters.

Colonies moderately growing, circular and relatively compact, umbonate, somehow felty and dry. Colony texture somewhat velvety and margins rather irregular to entire. Colonies flat on OA and a high number of pycnidia and micropycnidia were observed on OA and CMA. The mycelia grew deep into the agar when cultivated on CZ medium; a character which was not seen on the other media. A high number of pycnidia was found when the pH of the medium was as low as 2.5 up to 3.5, as observed on MEA medium. Pycnidia mostly solitary and rarely seen in clusters or in chains, on the surface and within the agar.

Colonies on MEA attaining a diam. of 20 mm within 14 days at 28 °C, butter milk-coloured (FEF1B5) to mandarin orange (E47833). Colonies on PDA attaining a diam. of 30 mm within 14 days at 28 °C, dark wood-coloured (855E42) to medium wood-coloured (A68064). Colonies on OA, coconut shell-coloured (BC7642), spreading broadly, attaining a diam. of 50 mm within 14 days at 28 °C. Colonies on CMA attaining a diam. of 20 mm within 14 days at 28 °C, mandarin orange (E47833). Colonies on DG18 attaining a diam. of 23 mm within 14 days at 28 °C, coconut shell-coloured (BC7642). Colonies on CYA attaining a diameter of 40 mm within 14 days at 28 °C, mandarin orange (E47833) to coconut shell-coloured (BC7642). The optimum pH and temperature for growth were recorded as 4.5 and 25 °C, respectively. Furthermore, 0.6% (w/v) NaCl was mostly favoured for growth, as assessed on MEA medium.

Physiology

According to the Lye test, strains IBRC-M 30051 and IBRC-M 30047 did not produce colourless antibiotic metabolite E.

No growth was detected on MEA at 10 °C and thus, 15 °C was recorded as the lowest temperature which support the strains’ growth. They did not grow at 40 °C. The optimum temperature for growth was 25 °C. As assessed on MEA medium, the lower and upper limits of tolerable pH values were 2.5 and 10, respectively. The optimum pH value for growth was around 4.5 ( Table 2 ). The greatest tolerable NaCl concentration was 10% (w/v) and the most preferred NaCl concentration was 0.6% (w/v) in MEA medium ( Table 2 ).