Diatrypella (Ces. & De Not.) De Not.

Rathnayaka, Achala R., Chethana, K. W. Thilini, Manowong, Areerat, Bhagya, Amuhenage T., Win, Hsan, Tun, Zaw L., Mapook, Ausana & Hyde, Kevin D., 2025, Taxonomy, phylogeny, and bioactive potential of Xylariales (Sordariomycetes, Ascomycota) from Thailand: novel species discovery, new host and geographical records, and antibacterial properties, MycoKeys 120, pp. 35-117 : 35-117

Diatrypella (Ces. & De Not.) De Not. , Sfer. Ital.: 29 (1863)

Notes.

Cesati and De Notaris (1863) introduced Diatrypella with D. verruciformis as the type species. This genus is characterized by stromata, which are conical to truncate, cushion-like or discoid, and usually delimited by a black zone within host tissues, umbilicate or sulcate ostiolar necks. Asci are cylindrical, polysporous, and long-stalked, and ascospores are hyaline to yellowish. Diatrypella species have a libertella-like coelomycete asexual morph ( Kirk et al. 2008; Hyde et al. 2020 a). There are 74 Diatrypella species in Species Fungorum (2024), and only 23 of them have sequence data.

Phylogenetic analysis for Diatrypaceae

The ITS and β-tub combined data set consists of 144 taxa representing strains of Diatrypaceae , including Kretzschmaria deusta ( CBS 826.72 ) and Xylaria hypoxylon ( CBS 122620 ) as the outgroup taxa. The aligned data set comprises 1404 characters, including gaps (ITS = 504 bp, β-tub = 897 bp). The topology of the BI tree was similar to that of the ML tree. The best-scoring RAxML tree, with a final likelihood value of - 20102.616796, is shown in Fig. 17 . The matrix comprises 944 distinct alignment patterns, with 36.45 % undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.222804, C = 0.272806, G = 0.233404, T = 0.270985; substitution rates AC = 1.101521, AG = 3.499467, AT = 1.354291, CG = 0.845871, CT = 4.738241, GT = 1.0; gamma distribution shape parameter α = 0.360572. In the BI analysis, the average standard deviation of split frequencies was 0.009 after 9,000,000 generations of runs. The phylogenetic tree topology is similar to the study by Dissanayake et al. (2024). According to the phylogenetic analyses, our strains, MFLU 24-0533 and MFLU 24-0534 , formed a separate clade with Diatrypella oregonensis ( CA 117 and DPL 200 ), D. pseudooregonensis ( GMB 0041 and GMB 0040 ), and D. verruciformis ( UCROK 1467 and UCROK 754 ) with 93 % ML bootstrap and 0.94 pp, while MFLUCC 24-0614 clusters with Paraeutrypella citricola ( HVGRF 01 and HKAS 133111 ) with 99 % ML and 0.97 pp bootstrap support.

Figures

Figure 17. Phylogram generated from ML analysis based on the combined dataset of ITS and β-tub. The tree is rooted to Kretzschmaria deusta (CBS 826.72) and Xylaria hypoxylon (CBS 122620). Bootstrap support values for ML ≥ 70 % and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. Strains isolated in this study are presented in blue, and type strains are in bold.