Fusarium weifangense S. L. Han, M. M. Wang & L. Cai

Ai, Congcong, Liu, Qiyun, Wang, Yaling, Zhang, Zhaoxue, Li, Duhua, Geng, Yun, Zhang, Xiuguo & Xia, Jiwen, 2025, Morphological and phylogenetic analyses reveal new species and records of Fusarium (Nectriaceae, Hypocreales) from China, MycoKeys 116, pp. 53-71 : 53-71

Fusarium weifangense S. L. Han, M. M. Wang & L. Cai , Studies in Mycology 104: 87–148. 2023.

Fig. 4

Synonym.

Fusarium caulendophyticum H. Zhang & Y. L. Jiang , Mycosphere 14 (1): 2092–2207. 2023.

Description.

Conidiophores arising from aerial mycelium, 14–18 μm long, unbranched or irregularly branched, often reduced to single phialides; aerial phialides monophialidic, subulate to subcylindrical, smooth- and thin-walled, with inconspicuous or absent periclinal thickening, 9.2–12.2 × 4.0–4.4 μm; aerial conidia hyaline, rarely ellipsoidal to falcate, slightly curved with almost parallel sides, tapering towards both ends, with a blunt to conical and slightly curved apical cell, blunt to barely notched basal cell, smooth and thin-walled, (1 –) 3–5 - septate; 1 - septate conidia: (14 –) 15–19 (– 20) × 3–4 μm (av. 17 × 3 μm, n = 8); 2 - septate conidia: (19 –) 19–21 (– 24) × 3–5 μm (av. 21 × 4 μm, n = 14); 3 - septate conidia: (22 –) 26–31 (– 34) × 3–6 μm (av. 28 × 4 μm, n = 22); 4 - septate conidia: (30 –) 35–36 (– 45) × 3–6 μm (av. 36 × 5 μm, n = 17); 5 - septate conidia: (31 –) 34–37 (– 46) × 4–6 μm (av. 38 × 5 μm, n = 15). Sporodochia salmon to orange, formed abundantly on surface of carnation leaves. Sporodochial conidiophores densely, bearing apical whorls of one phialide; sporodochial phialides monophialidic, subulate to subcylindrical, 16–24 × 2–3 μm, smooth. Sporodochial conidia falcate, slightly curved, tapering towards both ends, with a slightly elongated conical or whip-like curved apical cell, a foot-like to notched basal cell, (0 –) 4–5 - septate, hyaline, thin, and smooth-walled; 0 - septate conidia: 26–28 × 3–4 μm; 1 - septate conidia: (17 –) 26–36 (– 37) × 3–6 μm (av. 28 × 4 μm, n = 10); 2 - septate conidia: (20 –) 21–37 × 3–5 μm (av. 25 × 4 μm, n = 7); 3 - septate conidia: 21–33 (– 38) × 3–5 μm (av. 32 × 5 μm, n = 12); 4 - septate conidia: (31 –) 32–35 (– 44) × 3–6 μm (av. 36 × 4 μm, n = 22); 5 - septate conidia: (34 –) 40–45 (– 48) × 3–6 μm (av. 42 × 4 μm, n = 16). Chlamydospores not observed.

Culture characteristics.

Colonies on PDA incubated at 25 ° C in the dark, reaching 86–90 mm diameter in 7 d; surface white, flat, felty to velvety, aerial mycelia dense, colony margin entire; reverse white, odor absent. Colonies on OA incubated at 25 ° C in the dark, reaching 85–89 mm diameter in 7 d; surface white and aerial mycelia scant, radiate, reverse white, radiate, odor absent.

Materials examined.

China • Sichuan Province, Baoting Li and Miao Autonomous County , on leaves of Prunus salicina , 2 July 2023, Q. Y. Liu ( HSAUP 20852 , HSAUP 30852 ), living cultures SAUCC 5208 C-2 = CGMCC 3.27939 , SAUCC 5208 C-3 .

Notes.

Fusarium weifangense ( LC 18333, ex-type strain) was proposed by Han et al. (2023). Fusarium caulendophyticum ( CGMCC 3.25474, ex-type strain) was proposed by Zhang et al. (2023 a). Fusarium weifangense ( LC 18333, ex-type strain) was the first to be discovered. Fusarium weifangense ( LC 18333 and LC 18243) are clustered with Fusarium caulendophyticum ( CGMCC 3.25474 and GUCC 191050.2) clade in the combined phylogenetic tree (Fig. 1 , Suppl. material 4). Fusarium weifangense ( LC 18333, ex-type strain) and Fusarium caulendophyticum ( CGMCC 3.25474, ex-type strain) were similar in cal (0 / 535), rpb 2 (1 / 657), and tef 1 (2 / 462) sequences. We therefore considered the Fusarium caulendophyticum synonym of Fusarium weifangense . In this study, our strains ( SAUCC 5208 C- 2 and SAUCC 5208 C- 3) are clustered with the Fusarium weifangense ( LC 18333 and LC 18243) clade in the combined phylogenetic tree (Fig. 1 ). SAUCC 5208 C- 2 and SAUCC 5208 C- 3 were similar to the latter in cal (with 100 % sequence identity), rpb 2 (99.85 %), and tef 1 (98.70 %) sequences. Fusarium weifangense was previously isolated from wheat, Capsicum sp. , Triticum sp. , Medicago sativa , Lactuca sativa , Chenopodium quinoa , and Rosaceae roxburghii , and it has now been reported for the first time on Prunus salicina ( Wang et al. 2019; Xia et al. 2019; Yin et al. 2021; Han et al. 2023; Zhang et al. 2023 a) (Suppl. material 3).

Figures

Figure 1. Phylogeny inferred based on the combined cal - rpb 2 - tef 1 sequence dataset of the Fusarium incarnatum-equiseti species complex (FIESC), with Fusarium concolor (NRRL 13459) as the outgroup. The RAxML Bootstrap support values (MLBS ≥ 70 %) and Bayesian posterior probabilities (BIPP ≥ 0.90) were shown at the nodes. Ex-type, ex-epitype, and ex-neotype strains were indicated by T, ET, and NT, respectively. Strains isolated in this study were indicated in red.

Figure 4. Fusarium weifangense (SAUCC 5208 C- 2) a colony on PDA after 7 days at 25 ° C (left: above, right: reverse) b colony on OA after 7 days at 25 ° C (left: above, right: reverse) c sporodochia on carnation leaves d polyphialide e monophialide f sporodochial conidiophores g sporodochial conidia h aerial conidia. Scale bars: 10 μm (d – h).