Deniquelata quercina A. Alidadi, M. Javan-Nikkhah & M. Kowsari, 2019

Alidadi, Amin, Kowsari, Mojegan, Javan-Nikkhah, Mohammad, Karami, Saadi, Ariyawansa, Hiran A. & Jouzani, Gholamreza S., 2019, Deniquelata quercina sp. nov.; a new endophyte species from Persian oak in Iran, Phytotaxa 405 (4), pp. 187-194 : 192

Deniquelata quercina A. Alidadi, M. Javan-Nikkhah & M. Kowsari , sp. nov. ( Fig. 2 )

MycoBank number MB 826956

Etymology:—the epithet was named based on the host genus from which the fungus was isolated.

Description:— Ascomata on OA after 25−35 days under 12/12 nUV/light at 25°C, solitary, globose to sub-globose, dark brown, covered with mycelial hairs, with a central, circular, papillate, depressed and wide opening ostiole with visible pore, exudate black spore matrix at maturity and 120−300 × 150−325 μm ( x = 270 × 285 μm, n = 20); hamathecium embedded in a gelatinous matrix, dense, hyaline, septate and 1−2 μm in diameter. Peridium composed of several layers (more than 10 layer) and two types of cells, 20−50 μm in diameter laterally, up to 70 μm thick at the apex; outer layer composed of brown to black thick-walled cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis. Asci 85−155 × 13−18 μm ( x =112.23 × 15.66 μm, n = 20), 8-spored, bitunicate with a 2−3 × 3−5 μm ocular chamber, fissitunicate, rounded at apex, cylindrical to cylindro-clavate, with long and broad furcate pedicel 17−48 μm ( x = 22.13, n = 20). Ascospores 9−11 × 18−21 μm ( x = 10.16 × 19.7 μm, n=20), oblong, ellipsoid, ovoid to fusoid, straight or slightly inequilateral, hyaline at first, yellow to yellowish brown when immature, becoming reddish-brown to black at maturity, with 3 transe septa (rarely 4) and 1−3 longitudinal septa, constricted at the septa, usually with the wide second cell, apical cell mostly aseptate (rarely with 1 longitudinal septum), uniseriate or overlapping biseriate in the middle to the top part of ascus and without mucilaginous sheath.

Cultural characteristics: Ascospores germinated on WA during 12 h and germ tubes produced from each cell ( Fig. 2P ). Colonies on MEA effuse, velvety, white to yellowish white, reaching to 54 mm diameter after 10 days at 25 °C ( Fig. 2A ). Colonies on OA reaching to 20 mm diameter after 10 days, brown in the center and white at the edge ( Fig 2B ). Colonies on PDA reached to 52 mm diameter after 10 days.

Material examined: Iran, Ilam Province, Tangeh Dalab region, Zagros forest, on branches of Quercus brantii ( Fagaceae ) September 2014, A. Alidadi 20SA (UTFC 852; holotype), extype living culture ( ABRIICC 10068); Ilam Province, Chaqa Sabz region, Zagros forest, on branches of Quercus brantii ( Fagaceae ) September 2014, A. Alidadi, 20SB (UTFC 853, paratype)—living culture ( ABRIICC 10111).

Figures

FIGURE 2. Deniquelata quercina (holotype). A–B. Surface of colonies on MEA and OA respectively C. Ascomata on Surface of OA D. Ascomata. E. Ostiole. F. Cellular and hyaline pseudoparaphyses G–H Section of peridium. I–L Eight-spored asci. M. pedicel N–P. Ascospores. Scale bars: D = 100 μm, E=50 μm, G–H = 20 μm, I–O and F = 10 μm.