Pseudorhypophila poae H. Liu & H. Sang, 2025

Liu, Haifeng, Choi, Hyeongju, Paul, Narayan Chandra, Ariyawansa, Hiran A. & Sang, Hyunkyu, 2025, Discovering fungal communities in roots of Zoysia japonica and characterising novel species and their antifungal activities, IMA Fungus 16, pp. e 138479-e 138479 : e138479-

Pseudorhypophila poae H. Liu & H. Sang sp. nov.

Fig. 18

Etymology.

Name refers to its host family Poaceae.

Description from living culture CMML 20-36 :

Sexual morph: undetermined. Asexual morph: Sporulation abundant on MEA. Conidiophore erect, 1.5–2.5 μm in width, Conidia solitary or in clusters, pyriform, obovoid or triangular, 4.2–5.6 × 2.5–4.5 μm (Fig. 18 b – e ).

Type.

Korea • South Jeolla Province, Hwasun , isolated from roots of Zoysia japonica , October 2020, H. Liu and H. Sang, holotype CMML 20-36 H (permanently preserved in a metabolically inactive state), ex-holotype CMML 20-36 , ex-isotype CMML 20-89 .

Culture characteristics.

Colony reaching 82.88 mm diam. on PDA after 7 days in darkness at 25 ° C, white to buff in both front and reverse sides (Fig. 18 a ).

Notes.

The genus Pseudorhypophila was recently introduced by accommodating four species including Triangularia mangenotii , Zopfiella marina , Z. pilifera and Z. submersa ( Harms et al. 2021) . In phylogenetic analysis using combined sequences of ITS, LSU, RPB 2 and TUB 2, strains in the present study CMML 20-36 and CMML 20-89 formed a single clade in the genus Pseudorhypophila supported with a high statistical support (100 % / 1.00) close to clade comprising ex-type strains of P. pilifera ( CBS 413.73 ) and P. mangenotii ( CBS 419.67 ) (Fig. 19 ). However, both P. pilifera and P. mangenotii produce sexual morph, which was not observed in strains CMML 20-36 and CMML 20-89 . In addition, these two strains differ from P. mangenotii in producing conidia singly or in clusters, whereas the latter produces conidia singly ( Harms et al. 2021). Therefore, based on phylogenetic analysis and morphological characteristics, Pseudorhypophila poae sp. nov. was introduced in this study.

Figures

Figure 18. Pseudorhypophila poae sp. nov. (CMML 20-36) a front and reverse sides of colony on PDA b – d conidiophores and conidia e conidia. Scale bars: 10 μm (b – d); 8 μm (e).

Figure 19. Maximum Likelihood phylogenetic tree based on combined sequences of ITS, LSU, RPB 2 and TUB 2 from Pseudorhypophila and relevant genera. Bootstrap values (BS) and Bayesian posterior probabilities (PP) are given at the nodes (BS / PP). Strains obtained from this study are in bold blue. Ex-type isolates are marked with T. Lasiosphaeris hirsuta (SMH 1543) was used as the outgroup taxon.