Strumigenys lojanensis, Lattke & Aguirre, 2015

Lattke, John E. & Aguirre, Nikolay, 2015, Two New Strumigenys F. Smith (Hymenoptera: Formicidae: Myrmicinae) from Montane Forests of Ecuador, Sociobiology 62 (2), pp. 175-180 : 176-178

Strumigenys lojanensis n. sp.

Figs 1 – 3. Strumigenys lojanensis n. sp. Worker. Scale bar =

0.25mm. Pilosity omitted.

Fig 1. Strumigenys lojanensis n. sp., head dorsal view.

Fig 2. Strumigenys lojanensis n. sp., head lateral view.

Fig 3. Strumigenys lojanensis n. sp., body lateral view. Elongated shape on mesopleuron outlined by broken line defines area of smooth and shining sculpturing.

JE Lattke and N Aguirre – Two new Strumigenys species from Ecuador

Diagnosis. Mandibles straight with small relatively samesized 5-7 preapical denticles along anterior half; mandibular apex with two minute intercalary denticles that appear midway between upper and ventral apical teeth. Disc of postpetiolar dorsum weakly reticulate-punctate, spongiform processes of postpetiole well-developed. Head, most of mesosoma, coxae, femora and tibiae densely reticulate-punctate; katepisternum mostly smooth and shining.

Type Material. Holotype. Ecuador, Loja, Reserva El Madrigal, 4.04655°S 79.17583°W, 6.3 km SSE of Loja, 2350m, 28 August 2014, J. Lattke 3590-14. Holotype (worker) deposited in QCAZ . Paratypes. (1) One worker and one queen from the same sample as the holotype also deposited in QCAZ . (2) Same locality data and date as the holotype: sample Lattke 3590- 15 1w in RBINS . (3) Same locality data and date as the holotype: sample Lattke 3590- 03 1w in DZUP, 1w in ICN, 1w in MCZC, 1w in MZSP . (4) Ecuador, Loja, Estación Fundación Arco Iris, Loja – Zamora road, km 23 , 3.98846°S 79.09326°W, 12.12k E of Loja, 2105m, 18 August 2014, J. Lattke 3573. From sifted leaf litter sample. One dealate queen and 1 worker deposited in CISEC .

Worker description. Holotype (Paratypes, n=4): HL 0.62(0.60-0.63), HW 0.47(0.46-0.50), ML 0.49(0.48-0.50), SL 0.43(0.40-0.43), PW 0.31(0.31-0.34), AL 0.67(0.66-0.69) mm; CI 76(74-79), MI 79(76-82), SI 91(0.82-93). Posterior cephalic margin deeply concave, occipital lobes well developed; surface of cephalic dorsum transversely convex; anterior clypeal margin transverse, slightly sinuate with low median convex lobe. Dorsolateral margin of head with flagellate hair just posterad of eye level; cephalic dorsum with two pairs of slightly spatulate erect hairs, highest pair close to posterior cephalic border whilst shorter pair close to eye level. Leading edge of scape with slender hair at base that arches apicad, followed by 5 thicker, spatulate hairs: second hair from base arches apicad and is more slender than hairs 3-6, hairs 3-4 arch basad and hairs 5-6 usually arch apicad, though some specimens may have hair 6 arching towards base. Apical lobes of labrum clearly visible with mandibles closed, short and converge towards each other, slightly wider at base than at blunt apex; trigger hairs extend anterad to midpoint of mandibular length. Mandible straight, external margin straight to weakly convex, internal margin weakly sinuate, basally convex and apically concave, basal lamella convex; preapical denticles 5-6 on left mandible and 5-7 on right mandible with mandible pointing towards observer; denticles closest to apex usually larger though minute denticles or stubs may be present. Mandibular apex with two minute intercalary denticles that appear midway between upper and ventral apical teeth.

Mesosoma in lateral view with pronotal margin broadly convex, mesonotal to propodeal margin relatively flat. Transverse section of pronotal dorsum broadly convex; pronotal humeral hair fine and flagellate, sometimes forming apical loop; mesonotum with single pair of flagellate hairs; propodeal spiracle separated from posterior edge of propodeal lamella by over single

Sociobiology 62(2): 175-180 (June, 2015) 177

width; propodeal tooth triangular, apex pointed. Propodeal tooth triangular, sharply pointed and slightly higher than basal length; metapelural lobe bluntly triangular, not higher than propodeal tooth.Petiole lacking spongiform processes, ventrum with low anterior lamella that extends posterad to midlength; spongiform sculpturing well-developed on postpetiolar ventrum and posterolaterally, vestigial strands of spongiform sculpturing usually present along anterior margin of abdominal sternite IV. Postpetiolar tergite with narrow lamella along anterior margin. Dorsum of abdominal segments II – V with series of straight to weakly arched hairs, all semi-erect over cuticular surface. Petiolar node with posterior pair, postpetiole with 3 pairs, two dorsal pairs and a lateral pair. Anterior border of abdominal tergite IV with transverse arched crest that parallels posteriorly projecting lamella of postpetiole, a few brief longitudinal costulae may be present along this crest; most of tergite smooth and shining with 20-24 erect, slightly arched, spatulate hairs and no appressed pubescence.

Head, most of mesosoma, petiole, postpetiole, coxae, femora and tibiae densely reticulate-punctate, sculpturing attenuated on discal area of postpetiolar node and along its anterior margin; katepisternum mostly smooth and shining with strip of reticulate-punctate sculpture along anteroventral and ventral margins, propodeal declivity mostly smooth and shining posteriorly; abdominal tergite IV smooth and shining. Body mostly dark brown, almost black; mandibles, antennae, and legs brown.

Gyne. (Gyne 1 - Gyne 2): HL(0.62-0.64), HW(0.5-0.51), ML(0.51-0.48), SL(0.41-0.41), PW(0.38-0.39), AL(0.77-0.77) mm; CI(81-80), MI(82-75), SI(82-80). Besides the expected morphological differences the gyne is quite comparable to the workers except for the better developed petiolar ventral lamella, although still low it bears more resemblance to spongiform sculpturing whilst in the worker it is simply a brief, low lamella.

Etymology. The specific epithet “lojanensis ” is derived from the name of the Ecuadorian state in which the specimens were found, Loja.

Discussion. This species fits quite well within the gundlachi complex as defined by Bolton (2000:176) but the spongiformprocessesofthepostpetiolearemuchmoredeveloped in S. lojanensis . Within the gundlachi complex, S. lojanensis fits comfortably into the gundlachi species cluster, with the only difference consisting in the weaker reticulate sculpturing of the postpetiolar dorsum. Using the key to Neotropical Pyramica in Bolton (2000:137) specimens of S. lojanensis will flow directly to S. enopla (Bolton). Using the description of S. enopla ( Bolton, 2000: 185) and images of a Paratype from Antweb (CASENT0900177) it was possible to identify a number of discrete differences between the two species: The cephalic subdecumbent pilosity is not as arched and high as in S. enopla; the subdecumbent hairs on the mesosomal dorsum are also higher and more arched in S. enopla, as well as the standing hairs on petiole, postpetiole and gaster. The mesosomal dorsal margin of S. lojanensis is straight in lateral view, whilst in S. enopla it

is distinctly sinuate and the propodeal denticles are stouter in S. lojanensis than in S. enopla. There are 2 standing hairs on the petiolar dorsum of S. lojanensis , compared with 4 in S. enopla. In S. enopla the posterior edge of the spongiform sculpturing on the postpetiole is convex, but in S. lojanensis there is a distinct low median lobe.

The reticulate-punctate sculpture of the postpetiole is mostly strong and comparable to that of the petiole, covering most of it but weakens posteromedially on the dorsum, so it could be difficult to decide which way to go at couplet 7 of Bolton’s key. If the user decides for first option then the specimen will become stranded at couplet 9 due to the number of denticles, exceeding the maximum amount for either option. If the modification of Bolton’s key proposed by Rigato and Scupola (2008: 481) is used, then the specimen will key directly to S. osellai (Rigato & Scupola). In this case differences in the preapical dentition and metapleural sculpturing will permit easy separation: S. lojanensis bears only small denticles and never a large pair of denticles, plus the metapleuron in S. osellai is smooth and shining, but not so in S. lojanensis .

The Arco Iris site is located within Podocarpus National Park along the road that joins Loja with Zamora within dense montane cloud forest, bearing a canopy not more than 20 m high upon steep slopes. It is within the catchment area of the Río San Francisco, with an estimated average annual rainfall of 3500-4000 mm, relative cloud cover of 70-80% (Richter et al. 2013), and an estimated mean annual temperature of 15-16°C ( Wilcke et al., 2008). The Reserva Madrigal locality is a private reserve that neighbors Podocarpus National Park approximately 7 km SSE from Loja. It was previously a dairy farm and has been undergoing restoration towards forest during the last ten years. The specimens were taken from several samples along a 200 m long transect in mostly secondary vegetation, ranging from trees approximately 15 m high forming a loose canopy to more open bracken (Pteridium sp.) dominated understory with scattered trees and shrubs. Neighboring slopes to the sampling site across the stream bear dense forest with a canopy apparently not more than 15 m high. The area has an estimated average annual rainfall of 3000- 2500 mm (Richter et al., 2013).

With the discovery of S. lojanensis it is possible to discern a group of 5 northern andean species of the gundlachi complex with a preference for cold forests above 2000 m altitude, where the presence of most ants is negligible ( Longino, 2014). The other species are S. enopla, known from altitudes between 1900 and 2200 m in SW Colombia, S. nubila Lattke and Goitía , sampled from altitudes between 2000 and 2500 m in Colombia and Venezuela, S. vartana (Bolton), a Colombian species known from altitudes between 1800 and 2530 m and S. heterodonta (Rigato & Scupola) which was recently described from 2940 m altitude in Ecuador ( Rigato & Scupola, 2008).

Are these cold weather specialists closely related? Do they form a monophyletic group? Bolton recognizes two complexes of species within the gundlachi -group based upon the mandibular dentition and the development of the apical lobes of the labrum

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and their accompanying trigger hairs: the crassicornis complex, and the gundlachi complex. Bolton (2000) informally divides the gundlachi complex into four clusters of apparently related species using the mandibular index, number and position of intercalary denticles of the mandibular fork, number and position of the preapical mandibular dentition, configuration of the main pilosity, and sculpture of the postpetiolar node. In his scheme both S. enopla and S. lojanensis fit in the gundlachi cluster, where they are the only cloud forest specialists, though widely distributed species such as S. denticulata (Mayr) , and S. gundlachi (Roger) also have broad altitudinal ranges that include cloud forests but none approaching 2000 m ( Bolton, 2000; Lattke & Goitia, 1997). S. vartana lies in the laevipleura cluster, which includes S. gemella Kempf recorded from cloud forests in Colombia close to 1700 m ( Bolton, 2000). S. nubila shares the nubila cluster along with S. lalassa (Bolton), a species found from sea level to 1500 m from Costa Rica to Ecuador ( Bolton, 2000). Rigato and Scupola (2008) described S. heterodonta as part of the gundlachi complex, and opted for placing it in fifth species cluster as they were not satisfied with its affinities to any of the other species. Nevertheless S. heterodonta is quite comparable with the nubila cluster as it fits most of the traits used by Bolton, the differences being more of a degree than categorical. Most of the seven species of the subedentata cluster are found in low to medium altitudes except for S. connectens (Kempf) , known from forests close to 1500 m in Ecuador ( Bolton, 2000), and S. paniaguae (Longino) found in Costa Rica between altitudes of 500-1500 m ( Longino, 2006). A brief overview of the ten species making up the crassicornis complex ( Bolton, 2000) shows most of them to inhabit low to medium altitudes except for S. pasisops (Bolton), found in cloud forests of 1500 m, so it seems that the cold weather (> 2000 m) specialists are, for the while being, restricted to the gundlachi complex, implying some close relationship.

A phylogenetic analysis is in order to get a better grip on the issue but it should include not only the species as they are presently known but also representative populations from different sites for each species, especially the more widespread ones with broad geographical and altitudinal coverage such as S. gundlachi or S. denticulata . Upon discussing morphological variability within S. gundlachi, Bolton (2000) points out the distinctness of some high altitude specimens from Colombia, which could represent a different species. A similar situation repeats itself when Bolton discusses variability within S. denticulata and some high altitude specimens from SW Colombia. Longino (2006) found and described three species apparently closely related to S. subedentata Mayr that form an elevational succession along the slopes of his sampling sites in Costa Rica with each occupying a determined altitudinal range. A similar situation is then conceivable for S. gundlachi and S. denticulata , or perhaps other species. More sampling and alpha taxonomy is needed, especially in the mountains of Colombia, Ecuador, Peru, and Bolivia, before we may have a better picture of the distribution and relations of the gundlachi complex cold weather specialists. Climate change and the ever increasing destruction and fragmentation of the Andean cloud forests actively work against such a research agenda.

JE Lattke and N Aguirre – Two new Strumigenys species from Ecuador