Bambusicola autumnalis R. R. Liang, S. N. Zhang & Jian K. Liu, 2023

Feng, Yao, Chen, Ya-Ya, Lin, Chuan-Gen, Liu, Zuo-Yi, Chen, Xiao-Fang, Pei, Guo-Shun & Liu, Jian-Kui, 2025, Morpho-phylogenetic evidence reveals novel Bambusicolous fungi from Guizhou Province, China, MycoKeys 118, pp. 81-103 : 81-103

Bambusicola autumnalis R. R. Liang, S. N. Zhang & Jian K. Liu , Phytotaxa 601 (3): 208 (2023)

Figs 6 , 7

Description.

Saprobic on dead bamboo culms. Sexual morph: Ascomata 263–370 μm diam., 185–202 μm high, solitary, scattered to gregarious, initially growing under the host surface, breaking through the surface after maturity, with cracks on the host, subglobose with a flattened base, some arranged in rows, brown to dark brown. Peridium comprising several layers of cells of textura angularis, with dark brown outer layers and hyaline inner layers. Hamathecium of dense, 1–2 μm wide, filamentous, branched, indistinctly septate, smooth-walled, trabeculate pseudoparaphyses, anastomosing at the apex, embedded in a hyaline, gelatinous matrix. Asci 64–95 (- 102) × (10 –) 11-14 μm (x ̄ = 83 × 12 μm, n = 20), 8 - spored, bitunicate, broadly cylindrical to cylindrical-clavate, with a short pedicel, apically rounded with a well-developed ocular chamber. Ascospores (24 –) 26–29 × 4–6 μm (x ̄ = 26 × 5 μm, n = 30), overlapping, biseriate, hyaline, fusiform, with acute ends, 1 - septate, slightly constricted at the septa, asymmetric, slightly curved, smooth-walled with 2–4 guttules, surrounded by a thin, inconspicuous mucilaginous sheath. Asexual morph: Coelomycetous, produced on PDA. Conidiomata pycnidial, solitary to gregarious, superficial. Conidiogenous cells 5–17 × 2–4 μm (x ̄ = 9 × 3 μm, n = 15), holoblastic, integrated or discrete, subcylindrical to cylindrical, hyaline, smooth, occasionally with an enlarged structure at the top. Conidia with two types, macro- and microconidia. Macroconidia 23–31 (– 34) × 4–6 (– 7) μm (x ̄ = 28 × 5 μm, n = 30), cylindrical to ellipsoidal, with rounded ends, 3 - septate, pale brown to brown, slightly constricted at the septa, smooth. Microconidia 3–6 × 1–2 (– 3) μm (x ̄ = 5 × 2 μm, n = 30), subglobose to ellipsoidal, with rounded to obtuse ends, pale brown, aseptate, smooth-walled.

Culture characteristics.

Colonies on PDA reaching 16 mm diam. after 10 d at 25 ° C in dark, white, circular, velvety, with uneven margin. Reverse white with a yellowish center.

Material examined.

China • Guizhou Province, Xingyi City . On dead bamboo stems, 5 September 2019, Yao Feng, XY 19-4 ( HKAS 112598 , GZAAS 21-0500 , living cultures CGMCC 3.20354 = GZCC 21-0800 ; ibid., 6 September 2019, Yao Feng, XY 19-1 ( GZAAS 21-0389 ), living culture GZCC 21-0897 .

Notes.

Liang et al. (2023) described Bambusicola autumnalis as a novel species based on its sexual morph and phylogenetic analysis. The phylogenetic results (Fig. 1 ) confirmed that our new collections are conspecific with B. autumnalis , and this study expands upon their findings by providing a detailed description of the asexual morph, thereby enhancing the taxonomic understanding of this species.

Figures

Figure 1. Phylogram generated from maximum likelihood analysis based on a combined dataset of SSU, ITS, LSU, rpb 2 and tef 1 - α sequences. Bootstrap support values for ML (≥ 75 %) and bayesian posterior probabilities (≥ 0.95) are given at the nodes (ML BS / PP). Branches with 100 % ML BS and 1.00 PP are thickened. New strains are shown in red. The tree is rooted with Sulcatispora berchemiae KT 1607 and Sulcatispora acerina KT 2982, and ex-type strains are in bold.

Figure 6. Bambusicola autumnalis (HKAS 112598) A, B appearance of ascomata on the host C pseudoparaphyses D vertical section of an ascoma E peridium F germinated ascospore G, H cultures on PDA G from above H from below I – M asci N – R ascospores. Scale bars: 100 µm (D); 25 µm (E, F); 20 µm (I – M); 15 µm (C, N – R).

Figure 7. Reproduced Bambusicola autumnalis (CGMCC 3.20354) A, B colony on PDA C-F conidiogenous cells with macroconidia G-K macroconidia L-N conidiogenous cells with microconidia O microconidia. Scale bars: 10 µm (C-O).