Dna extraction, , PCR

Li, Chuanhua, Zhang, Ming, Li, Jipeng & Li, Taihui, 2025, Volvopluteus deliciosus sp. nov. (Pluteaceae, Agaricales), a new edible species from Xinjiang, China, Phytotaxa 683 (3), pp. 253-262 : 254

DNA extraction, PCR amplification and sequencing

Genomic DNA was extracted from the dried specimens using the MagenHiPure Fungal DNA Mini kit (Magen Biotech Co., Ltd., Guangzhou) according to the manufacturer’s instructions. The sequences of the nuclear ribosomal internal transcribed spacer (nrITS) ribosomal RNA gene regions and the nuclear large subunit (nrLSU) ribosomal RNA gene regions were amplified by polymerase chain reaction using universal primers ITS1F & ITS4 ( White et al. 1990; Gardes & Bruns 1993) and LR0R & LR5 (https://sites.duke.edu/vilgalyslab/rdna_primers_for_fungi), respectively. Amplification reactions followed Zhang et al. (2022). Amplified products were sent to Beijing Genomic Institute for sequencing. The sequences were submitted to GenBank after being assembled.