Dna extraction

Atria, M., Eurlings, M., Baker, W. J., Dransfield, J. & Welzen, P. C. van, 2020, Phylogenetic analysis of the Calamus javensis complex (Arecaceae: Calamoideae) in Malesia, Blumea 65 (3), pp. 205-211 : 206

DNA extraction and amplification

All 53 samples were successfully amplified for 5S and 57 amplified for mat K. The designed primers resulted for mat K in sequences of 253 bp long and for 5S of 332 bp long. In the combined matrix there were 53 entities and 561 characters, of which 484 characters were constant, 53 were variable characters but parsimony-uninformative, and 24 characters were parsimony informative.