Halorosellinia Whalley, E. B. G. Jones, K. D. Hyde & Læssøe

Rathnayaka, Achala R., Chethana, K. W. Thilini, Manowong, Areerat, Bhagya, Amuhenage T., Win, Hsan, Tun, Zaw L., Mapook, Ausana & Hyde, Kevin D., 2025, Taxonomy, phylogeny, and bioactive potential of Xylariales (Sordariomycetes, Ascomycota) from Thailand: novel species discovery, new host and geographical records, and antibacterial properties, MycoKeys 120, pp. 35-117 : 35-117

Halorosellinia Whalley, E. B. G. Jones, K. D. Hyde & Læssøe , Mycol. Res. 104 (3): 368 (2000)

Notes.

Halorosellinia was introduced by Whalley et al. (1999) as a monotypic genus to accommodate H. oceanica (previously referred to as Hypoxylon oceanicum ). This genus is characterized by uniperitheciate ascomata immersed in a pseudostroma ( Hyde et al. 2016). Halorosellinia currently comprises five species ( Index Fungorum 2025). Only three Halorosellinia species are included in Wijayawardene et al. (2022), while five species are listed in the Index Fungorum (2025) and Hyde et al. (2024).

Phylogenetic analyses for Xylariaceae

For Xylariaceae , the ITS, rpb 2, and β-tub gene regions were used in the combined data set. Seventy-two isolates of Xylariaceae species were included in the analysis, with Hypoxylon fragiforme ( HAST 383 and MUCL 51264 ) as the outgroup taxa. After alignment, the dataset comprises 2747 characters, including gaps (ITS = 580 bp, rpb 2 = 1122 bp, β-tub = 1045 bp). The topology of the BI tree was similar to that of the ML tree. The best-scoring RAxML tree, with a final likelihood value of - 49702.0557, is shown in Fig. 10 . The matrix comprises 1637 distinct alignment patterns, with 16.93 % undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.243971, C = 0.271335, G = 0.241282, and T = 0.243412; substitution rates were AC = 1.350468, AG = 5.371719, AT = 1.169242, CG = 1.195494, CT = 7.102768, and GT = 1.0; and the gamma distribution shape parameter α = 0.335659. In the BI analysis, the average standard deviation of split frequencies was 0.01 after 3,000,000 generations of runs. The phylogenetic tree topology is similar to the study by Konta et al. (2020 a). According to the phylogenetic analyses, our strains ( MFLU 24-0536 and MFLUCC 25-0025 ) cluster with Halorosellinia xylocarpi ( MFLU 18-0545 ) with 100 % ML bootstrap and 1.00 PP support, while MFLUCC 24-0611 clusters sister to Stilbohypoxylon quisquiliarum ( YMJ 172 ) with 90 % ML bootstrap and 0.98 PP support (Fig. 10 ).

Figures

Figure 10. Phylogram generated from ML analysis based on the combined dataset of ITS, rpb 2, and β-tub. The tree is rooted to Hypoxylon fragiforme (HAST 383 and MUCL 51264). Bootstrap support values for ML ≥ 70 % and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. Strain numbers are noted after the species names. Strains isolated in this study are represented in blue, and type strains are in bold.