Data
publication ID |
https://doi.org/10.3767/000651916X691196 |
persistent identifier |
https://treatment.plazi.org/id/5E1B3975-FF9D-AC6A-4D79-3FE6FE9DF05E |
treatment provided by |
Felipe |
scientific name |
Data |
status |
|
Data View in CoL analysis
Complementary strands of the sequenced region were assembled using Gene Runner v. 3.05 (Hastings Software Inc., USA) and manually trimmed to obtain a clean sequence. The obtained sequences were deposited in GenBank. A homology check was carried out using NCBI BLAST program (http://blast. ncbi.nlm.nih.gov/Blast.cgi) to identify the presence of species with similar sequence combination. Nucleotide multiple align- ments were conducted with ClustalW embedded in MEGA6 ( Tamura et al. 2013). Genetic distances were generated using the Kimura 2-parameter model ( Kimura 1980), with all gaps treated as missing (complete deletion option). Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster trees were constructed using MEGA6, with bootstrap consensus of 1 000 replicates.
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