Kurtzmanomyces guiyangensis C. Y. Chai & F. L. Hui, 2025

Wang, Peng, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Two new species of Chionosphaera and Kurtzmanomyces (Chionosphaeraceae, Agaricostilbomycetes) isolated from China, MycoKeys 125, pp. 81-94 : 81-94

Kurtzmanomyces guiyangensis C. Y. Chai & F. L. Hui sp. nov.

Fig. 3

Etymology.

The specific epithet guiyangensis refers to the geographic origin of the ex-type strain, Guiyang City, Guizhou Province.

Typus.

China • Guizhou Prov.: Guiyang City, East Mountain Park , in the phylloplane of Debregeasia orientalis , August 2023, D. Lu, NYNU 23983 ( holotype GDMCC 2.499 T preserved as a metabolically inactive state, culture ex-type PYCC 9992 ) .

Description.

On YM agar after 7 days at 20 ° C, streak cultures are pale orange, smooth and butyrous, and the margin is entire. In YM broth after 3 days at 20 ° C, cells are spherical to ovoid (3.4–4.7 × 4.5–6.8 µm) and occur singly or in pairs. The conidiogenous stalks, normally one to three per cell, are short, 0.4–1.0 µm. After a month in YM broth at 17 ° C, a sediment and a ring are formed, but no pellicle is observed. After 7 days in Dalmau plate culture on CMA at 17 ° C, pseudohyphae are absent. Sexual structures are not observed on PDA, CMA or V 8 agar at 17 ° C. Ballistoconidia are not produced. Glucose fermentation is absent. The following carbon sources are assimilated: glucose, inulin, sucrose, trehalose, melezitose, salicin, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol, ribitol, galactitol, D-mannitol, D-glucitol, and succinate. Raffinose, melibiose, D-galactose, lactose, maltose, methyl α-D-glucoside, cellobiose, L-sorbose, L-rhamnose, D-xylose, methanol, glycerol, erythritol, myo - inositol, DL-lactate, citrate, D-glucuronate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate, and D-glucono- 1, 5 - lactone are not assimilated. Nitrate and nitrite are weakly assimilated as sole nitrogen sources. Ethylamine, l-lysine, and cadaverine are not assimilated. Growth is observed at 25 ° C, but not at 30 ° C. Growth in the presence of 0.01 % cycloheximide, 10 % NaCl plus 5 % glucose as well as 1 % acetic acid is negative. Growth in the vitamin-free medium is absent. Growth on 50 % (w / w) glucose-yeast extract agar is positive. Starch-like compounds are not synthesized. Diazonium blue B reactions is positive.

Additional strain examined.

China • Hainan Prov.: Wuzhishan City, Wuzhi Mountain , in the phylloplane of Glochidion sp. , July 2025, S. L. Lv, NYNU 25716 .

GenBank accession numbers.

Holotype GDMCC 2.499 T ( ITS: OR 961459 , LSU: OR 958742 , tef 1 α: PX 046187 ) ; additional strain NYNU 25716 ( ITS: PX 046462 , LSU: PX 046461 ; tef 1 α: PX 046188 ) .

Note.

Physiologically, K. guiyangensis sp. nov. differs from the closely related species M. nipponica in its ability to assimilate inulin, L-arabinose, D-arabinose, and D-glucitol and its inability to assimilate raffinose, galactose, maltose, and glycerol. Furthermore, M. nipponica is able to grow at 30 ° C, whereas K. guiyangensis sp. nov. does not exhibit growth at this temperature. A comparative summary of the distinguishing physiological characteristics between K. guiyangensis sp. nov. and other Kurtzmanomyces species is presented in Table 2 .

Figures

Figure 3. Morphology of Kurtzmanomyces guiyangensis (NYNU 23983). A. Colony on YM agar after 7 days at 20 ° C; B. Budding cells in YM broth after 3 days at 20 ° C.