Phyllosticta capitalensis Henn.

Zhang, Meng-Yuan, Zhang, Zhao-Xue, Li, Du-Hua, Zhang, Xiu-Guo, Xia, Ji-Wen & Li, Zhuang, 2025, Morphological and phylogenetic analysis reveal three new species Phyllosticta (Phyllostictaceae, Botryosphaeriales) in China, MycoKeys 118, pp. 35-54 : 35-54

Phyllosticta capitalensis Henn. , Hedwigia 48: 13 (1908)

Fig. 2

Description.

Leaf endogenic and associated with leaves of Mangifera indica . Sexual morph: Not observed. Asexual morph: Conidiomata pycnidial, mostly aggregated in clusters, black, erumpent. In PDA culture, exuding colorless to opaque conidial masses within 12 d or longer. Pycnidial walls multilayered, textura angularis, brown, inner walls of hyaline. Conidiophores indistinct, often reduced to conidiogenous cells. Conidiogenous cells 6–12 × 2.2–2.8 μm, subcylindrical, ampulliform, hyaline, smooth. Conidia 6.7–12 × 3.6–5.8 μm (L / W 1.42 –1.53), ovoid, ampulliform, ellipsoidal to subglobose, hyaline, aseptate, thin and smooth walled, multi-guttulate, or with a single large central guttule, surrounded by a mucilaginous sheath. Sheath 2.6–2.9 μm thick, thicker on both sides, and bearing a hyaline, apical mucoid appendage. Appendages 5.7–6.7 × 1.2–2.1 μm, flexible, unbranched, tapering towards an acutely rounded tip.

Culture characteristics.

Colonies on PDA 46–52 mm in diameter after 14 d at 25 ° C in darkness, with a growth rate of 3.3–3.7 mm / day, undulate at edge, grey white to greenish-black on obverse and reverse.

Additional specimen examined.

China • Hainan Province, Wanning City, Xinglong tropical botanical garden , on diseased leaves of Mangifera indica L., 28 March 2024, M. Y. Zhang ( HSAUP 7403 ), living culture CGMCC 3.28668 ; ibid., Z. X. Zhang ( HSAUP 7400 ), living culture SAUCC 7400–1 .

Notes.

The holotype ( CBS 128856 ) of P. capitalensis was collected from Stanhopea graveolens ( Glienke et al. 2011) . Two isolates ( CGMCC 3.28668 and SAUCC 7400 –1), collected from diseased leaves of Mangifera indica , cluster in the P. capitalensis clade (Fig. 1 ). In morphology, they possess the same morphological characters, such as subcylindrical to ampullate conidiogenous cells (6–12 × 2.2–2.8 vs. 7–10 × 3–5 μm), ellipsoidal to subglobose conidia (6.7–12 × 3.6–5.8 vs. 11–12 × 6–7 μm), and hyaline, apical mucoid appendages (5.7–6.7 × 1.2–2.1 vs. 6–8 × 1–1.5 μm). Therefore, we defined these two isolates as P. capitalensis .

Figures

Figure 1. Phylogram of the Phyllosticta, inferred from a concatenated alignment of ITS, LSU, tef 1, ACT, and GPDH sequences. Botryosphaeria obtusa (CMW 8232) and B. stevensii (CBS 112553) were used as outgroup taxa. BI posterior probabilities and ML bootstrap support values above 0.90 and 70 % are shown at the first and second positions, respectively. Ex-type cultures are highlighted in bold, while strains obtained in this study are marked in red. Some branches have been shortened for layout optimization, indicated by double diagonal lines with the corresponding reduction factor. The scale bar at the bottom left represents the number of substitutions per site.

Figure 2. Phyllosticta capitalensis (CGMCC 3.28668) a diseased leaf of Mangifera indica b, c colonies (left-above, right-reverse) after 14 d on PDA d conidiomata e, f conidiogenous cells with conidia g, h conidia. Scale bars: 10 μm (e – h).