Phytomyza pubicornis Hendel

Guglya, Yuliia, 2025, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2, Zootaxa 5658 (1), pp. 1-86 : 42-44

Phytomyza pubicornis Hendel

( Figs. 81–84 , 299–303 )

Material examined. Ukraine: Transcarpathia: Rakhiv , 430 m a. s. l., 48°03'N, 24°13"E, 8.v.2018 —mine collected, 11.v.2018 —pupation outside the mine, Yu. Guglya, ex Aegopodium podagraria (1♂) ; Kyiv Region: Rzhyshchiv , 49°58'25"N, 31°06'20"E, 21.v.2021 —mines collected, pupation outside the mine, x.2021 – ii.2022 —overwintered, 5– 8.ii.2022 —imago, Yu. Guglya, ex Aegopodium podagraria (2♀ 3 puparia); Kharkiv Region: Sharivka, 50°01'13"N 35°26'52"E, 10.v.2016 —pupation outside the mine, Yu. Guglya, ex Aegopodium podagraria (1♂ 2♀) .

Host: Apiaceae : Aegopodium podagraria L. ( Spencer 1976).

Mine. ( Fig. 81 ) The solitary larva forms a greenish or yellowish linear upper surface leaf mine, with grains and narrow lines of frass arranged irregularly along the margins irregularly; the third instar larva forms a very wide linear mine, that resembles a blotch. Pupation outside the mine.

Puparium. ( Figs. 82–84 ) Pale brown, glossy, 2.2 mm long, with distinct but shallow segmentation; surface quite smooth except the narrow bands of fine spines between all segments. Both pairs of spiracles black. Posterior spiracles set flat on the body cuticle and entirely separate; with 23–27 fine sessile bulbs in a kidney-shaped configuration. Two tiny sharp black appendages develope on each side of anal plate. Anal plate slightly protruding ventro-posteriorly viewed from the side.

Cephalopharingeal skeleton. ( Fig. 299a, b ) Right mouthhook larger dorsally and narrower than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks bear two sharp accessory teeth which are uniformly curved and directed ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua much less so. Intermediate sclerite straight; bearing one sharp tooth on the posterior third of ventral margin directed ventrally. Ventral cornu bears long “closed” window posteriorly. Indentation index 84.

Female genitalia. ( Figs. 300–303 ) Spermathecae relatively large, 0.31× as high as anterior part of oviscape. Proctiger generally cylindrical, slightly curve and concaved posteriorly; with two pairs medium setae located on posterior margin between cerci. Cerci distinctly spaced from each other. Cercus wide oval, flattened apically, 0.33× as long as proctiger; medium and short setae located subapically and apically. Spermathecae equal in size, brown, with wrinkled surface, squared. Internal duct invagination cylindrical, 0.8× as deep as height of spermatheca. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, S-shaped, with well sclerotized tail that is bowl-shaped in basal half. Body of receptacle squared, with acutely curved basal connecting tube, strongly sclerotized. Opening large, equal to the diameter of spherical part of the body, located on wide bowl-shaped projection, 1.04× as wide as a diameter of spherical part of the body. Basal connecting tube at the confluence of the tail with finger-shaped appendage, 2.0× as wide as width of the tail.

Distribution.West Palaearctic species recorded from 20 countries ( Papp & Černý 2019). Ukraine (first record).

Comments. There are 5 frorbs in all males and 1 female reared in Ukraine. Spencer (1976) and Papp & Černý (2019) noted 2 ors and 2 ori.

Figures

FIGURES 78–84. Figures 78–80: Phytomyza flavicornis Fallén; 78: empty puparium viewed from the side; 79: posterior segments of puparium (posterior view); 80: posterior spiracles (posterior view). Figures 81–84: Phytomyza pubicornis Hendel; 81: mine in Aegopodium podagraria leaf; 82: puparium viewed from the side; 83: posterior segments of puparium (posterior view); 84: posterior spiracles (posterior view).

FIGURES 299–305. Figures 299–303: Phytomyza pubicornis Hendel; 299 a, b: cephalopharingeal skeleton; a: total viewed from the side; b: right mouthhook; 300: oviscape, ventral receptacle and spermathecae proportionally; 301: spermathecae; 302: ventral receptacle; 303 a, b: proctiger; a: viewed from above; b: viewed from below. Figures 304–305: Phytomyza serriola sp. nov. 304: frontal view of head; 305: head viewed from above.