Agromyza nana Meigen

Guglya, Yuliia, 2025, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2, Zootaxa 5658 (1), pp. 1-86 : 9

Agromyza nana Meigen

( Figs. 8–10 , 107–113 )

Material examined. Ukraine: Transcarpathia: 6 km SE Luhy, 760 m a. s. l., 48°01'45"N, 24°30'59"E, 12.viii.2019 — mine with larva collected, Yu. Guglya, ex Trifolium repens (2 puparia); Ivano-Frankivsk Region: Chornohory Massif, 1439 m a. s. l., 48°9'N, 24°32'E, 9.vi.2018, Yu. Guglya (1♂); Volyn Region: Ostroviye, 51°33'06"N, 23°47'24"E, 24.v.2019, Yu. Guglya (2♂); Vinnytsa Region: near Olhopil, 48°07'42"N, 29°39'25"E, 11.v.2019 — pupation outside the mine, Yu. Guglya, ex Trifolium repens (2♂ 3 puparia); Poltava Region: near Luchky, 48°57'N, 34°9'E, 16.v.2016, Yu. Guglya (1♀); Kharkiv Region: Kharkiv, Sokolnyky, 50°01'41"N, 36°15'35"E, 17.vi.2011 — pupation outside the mine, Yu. Guglya, ex Trifolium repens (3puparia); Donetsk Region: Kryva Luka, 48°52'34"N, 37°54'37"E, 2.vi.2019, Yu. Guglya (1♀). Other material listed in Guglya (2011, 2013, 2015, 2017, 2020).

Hosts. Fabaceae : Anthyllis L., Medicago L., Melilotus Mill. , Onobrychis Mill. , Trifolium Tourn ex L., Trigonella L., Vicia L.; Oxalidaceae : Oxalis L. ( Benavent-Corai et al. 2005).

Mine. ( Fig. 8 ) The solitary larva develops in a white blister-blotch mine in the center of the leaf. Pupation takes place outside the mine in the soil.

Puparium. ( Figs. 9, 10 ) Yellowish-brown, matt, 3.0 mm, with deep segmentation; surface uniformly covered with numerous fine brown spines, posterior spiracle set on a conical protuberations in a triangular configuration. Ventral portion of last abdominal segment not protruding posteriorly viewed from the side. Anal plate brown and directed ventrally. Two oval, yellowish-brown appendages developed between the anal plate and posterior spiracles viewed from the side and posteriorly.

Cephalopharingeal skeleton. ( Fig. 110 ) Left mouthhook is slightly larger ventrally than the right, both with ventro-anterior portion wide and triangular. Both mouthhooks bear two accessory teeth; both dorsal and right ventral teeth long and strong, uniformly curved ventrally; left medial tooth much shorter, triangular. Parastomal bar is slender, 0.5× as long as intermediate sclerite. Mouthhooks and intermediate sclerite are strongly sclerotized, parastomal bar, dorsal cornua and ventral cornu much less so. Intermediate sclerite centrally curved, bearing sharp tooth directed posteriorly and located on the middle of ventral side. Indentation index 87.

Female head. ( Figs. 107, 108 ) Bicolour; ocellar tubercle, hind margin, 1 st fl, palps are black, the remain dark orange. I vt s and o vt s both in black ground. 1 st fl bearing with orange elongated and dorsally curved hairs. 1 st fl in male larger than in female.

Wing. ( Fig. 109 ) Hyaline, with beige undertone and brownish veins; costa ending after R 3+4; last section of CuA 1 slightly shorter than penultimate; calypter and margin pale brown, fringe brown. Wing length 2.5 mm.

Female genitalia. ( Figs. 111–113 ) Capsule of spermatheca medium sized, 0.3–0.4× as high as anterior part of oviscape. Spermathecae unequal in size, brown, oval. Neck of spermatheca is barrel-shaped, 0.3–0.4× as long as height of spermathecae. Spermathecal duct is very weakly sclerotized. Proctiger is wide, 2.4× as long as maximum width; more sclerotized posteriorly; all setae strong.

Distribution. Widespread and abundant European species, reported also from Oriental region ( Papp & Černý 2015).

Comments. Adults of Agromyza nana were captured in Ukraine from 1 May to 28 August evenly throughout the season.

Figures

FIGURES 8–12. Figures 8–10: Agromyza nana Meigen; 8: mine in Trifolium repens leaf; 9: puparium viewed from the side; 10: posterior segments of puparium (posterior view). Figures 11–12: Melanagromyza stemivora sp. nov.; 11: empty puparium viewed from the side; 12: posterior spiracles (posterior view).

FIGURES 107–113. Agromyza nana Meigen; 107: frontal view of head; 108: head viewed from the side; 109: wing; 110: cephalopharingeal skeleton; 111: oviscape and spermathecae proportionally; 112: spermathecae; 113: proctiger.