Amauromyza (Amauromyza) rameli Černý

Guglya, Yuliia, 2025, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2, Zootaxa 5658 (1), pp. 1-86 : 16-18

Amauromyza (Amauromyza) rameli Černý

( Figs. 25–27 , 155–161 )

Material examined. Ukraine: Kyiv Region: Rzhyshchiv,the River Dnipro Bank , 49°58'39"N, 31°06'18"E, 24.vi.2021, 26.vi.2021 —pupation, 8.vii.2021 —imago, Yu. Guglya, ex Lycopus europaeus (1♂, 4 puparia); Kharkiv Region: Pisochyn, the River Merla Bank , 49°57'58"N, 36°05'13"E, 29.vi.2024 —mines with larva collected, 11.vii.2024 — imago, Yu. Guglya, ex Lycopus europaeus (3♀) .

Hosts. Lamiaceae : Lycopus europaeus L.—the first recorded host plant.

Mine.( Fig.25 )The larva forms a brown blotch mine (not apical or marginal as in Phytomyza lycopi Nowakowski !), visibly dried. Pupation takes place either within or outside the mine attached to the leaf surface, but either case on the underside of the leaf. When pupation takes place inside the mine the larva still makes an exit slit.

Puparium. ( Figs. 26, 27 ) Orange-brown, glossy, 2.0 mm long, with deep segmentation; surface quite smooth except for narrow brown spine bands and two wrinkled last abdominal segments. Posterior spiracles set on stout conical protuberances that are strongly separated; with three hook-like sessile bulbs set in a circular configuration. Anal plate distinctly protruding above the surface of the puparium viewed from the side; directed ventrally.

Cephalopharingeal skeleton. ( Fig. 158 ) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally.Both mouthhooks with two accessory teeth; all sharp, uniformly curved and directed ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal cornu very weakly so. Ventral cornu very weakly sclerotized; bears large “closed” window medially. Intermediate sclerite straight, uniformly widening posteriorly; bears sharp tooth on the posterior third of ventral margin directed ventrally. Indentation index 80.

Female head. ( Figs. 155, 156 ) Black, only frons and gena dark brown; fronto-orbital plate slightly visible laterally; 2 ors, 2 ori; 1 st fl of medium size, round, flattened apically; bears short white pubescence. Gena wider at rear, in widest part 0.32× as wide as maximum height of eye. Vibrissa short, straight, directed anteriorly, 0.25× as long as maximum height of eye.

Wing. ( Fig. 157 ) Hyaline, with costa black, the remaining veins brown; costa reaching M 1; last section of CuA 1 2.38× as long as penultimate; calypter grey, margin and fringe black. Wing length 2.0 mm.

Female genitalia. ( Figs. 159–161 ) Spermathecae of medium size, 0.34× as wide as height of anterior part of oviscape. Proctiger relatively wide, 2.4× as long as maximum width; with cylindrical narrowing dramatically on posterior half; anterior half narrow, cylindrical, 0.12× as wide as posterior half; two pairs of long setae located on posterior margin between cerci. Cercus oval, slightly flattened apically, 0.24× as long as proctiger; patches of long and short setae located apically, laterally and ventrally. Spermathecae equal in size, brown, wider than high, flattened basally and convex apically. Internal duct invagination wider than high, 0.68× as deep as height of spermatheca, narrowing medially. Spermathecal duct weakly sclerotized.

Distribution. Greece, Hungary ( Papp & Černý 2016). Ukraine (first record).

Comments. In the end of June 2024 numerous mines with larvae of Amauromyza rameli and Phytomyza lycopi were collected together in Lycopus europaeus thickets. Most of them belonged to P. lycopi and were moustly already empty. In the end of June and in the end of July 2021 mines with larvae and puparia of P. lycopi were collected on Lycopus europaeus in the same location. Mines of A. rameli and P. lycopi can be easily separated from each other based on the position in the leaf. Both species forms visibly dried mine, but in P. lycopi it arranges along leaf margin, and in A. rameli not. (see Figs. 25, 28, 29 ). Puparia are rather different too. In A. rameli puparium is orange-brown, opaque, posterior spiracles with three hook-like sessile bulbs, and in P. lycopi puparium straw-coloured, transparent with black, two-horned posterior spiracles. (see Figs. 26, 27 and Figs. 213–215 in Guglya (2021)). So, one generation of A. rameli and two generations of P. lycopi were observed in Ukraine to date.

Figures

FIGURES 25–32. Figures 25–27: Amauromyza (Amauromyza) rameli Černý; 25: mine in Lycopus europaeus leaf; 26: empty puparium viewed from the side; 27: posterior segments of puparium (posterior view). Figures 28, 29: Phytomyza lycopi Nowakowski; 28: mine with larva in Lycopus europaeus leaf (view from upper side); 29: mine with larva in Lycopus europaeus leaf (view from lowerside).Figures 30–32: Amauromyza (Cephalomyza) gyrans (Fallén); 30: mine in Campanula rapunculoides leaf; 31: empty puparium viewed from the side; 32: posterior segments of puparium (posterior view).

FIGURES 155–161. Amauromyza (A.) rameli Černý; 155: frontal view of head; 156: head viewed from the side; 157: wing; 158: cephalopharingeal skeleton; 159: oviscape, proctiger and spermathecae proportionally; 160: spermatheca; 161: proctiger.

FIGURES 211–218. Phytomyza aconiti Hendel; 211: frontal view of head; 212: head viewed from the side; 213: wing; 214 a, b: cephalopharingeal skeleton; a: total viewed from above; b: mouthhooks viewed from the side; 215: oviscape, proctiger and spermathecae proportionally; 216: spermathecae; 217: proctiger; 218: ventral receptacle.