Phytomyza agromyzina Meigen

Guglya, Yuliia, 2025, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2, Zootaxa 5658 (1), pp. 1-86 : 30-31

Phytomyza agromyzina Meigen

( Figs. 55–58 , 219–226 )

Material examined. Ukraine: Kyiv Region: S Rzhyshchiv, 49°56'07"N, 31°02'50"E, 25.vi.2021 —mines with puparia collected, 4 and 6.vii.2021 —imago, Yu. Guglya, ex Cornus sanguinea (2♀); near Rzhyshchiv, ecostation “Hlyboki Balyky”, 49°57'44"N, 31°07'08"E, 23.vii.2021 —mines with puparia collected, 4 and 20.viii.2021 —imago, Yu. Guglya, ex Cornus sanguinea (3♀) .

In addition empty mines on Cornus sanguinea were collected in Ukraine and preserved in the herbarium from: Transcarpathia: between Kamianytsa and Antalivska Polyana, 48°40'20"N, 22°25'43"E, 410 m a.s.l., 26.vi.2018 — empty fresh mines collected, Yu. Guglya, ex Cornus sanguinea (4 mines); Vinnytsa Region: near Chechelnyk, 48°10'N 29°20'E, 1.vii.2019 —empty fresh mine collected, Yu. Guglya, ex Cornus sanguinea (1 mine); Kyiv Region: near Ulianyky, 49°54'50"N 31°07'28"E, 20.vii.2021 —empty fresh mine collected, Yu. Guglya, ex Cornus sanguinea (3 mines); near Rzhyshchiv, ecostation “Hlyboki Balyky”, 49°57'44"N, 31°07'08"E, 28.viii.2021 —empty fresh mine collected, Yu. Guglya, ex Cornus sanguinea (1 mine); Kharkiv Region: Kharkiv, Botanical Garden, 50°01'46"N 36°14'02"E, 10.vi.2012 —empty dried mine collected, Yu. Guglya, ex Cornus sanguinea (1 mine).

Hosts. Cornaceae : Cornus sanguinea L. and others Cornus spp. ( Spencer 1976).

Mine. ( Figs. 55, 56 ) The solitary larva forms a greenish, wide linear, upper surface leaf mine. Several larvae can feed in one leaf at the same time, but the mine do not merge and stay distinct. Pupation takes place either within or outside the mine. Also see comments below.

Puparium. (Figs. 57,58) Orange, silky-shining, with black, glossy posterior spiracles, 1.8 mm long, with shallow but distinct segmentation; surface finely wrinkled except for wide bands of fine spines. Posterior spiracles set on short stout separate conical protuberances; with 11–14 fine black sessile bulbs set in a kidney-shaped configuration. Anal plate orange, scarcely protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly.

Cephalopharingeal skeleton. ( Fig. 222a, b ) Mouthhooks equal in size, with ventro-anterior portion acute and abducted anteriorly. Right mouthhook with rounded posterior margin; bears distinct acute dorsal tooth and scarcely expressed ventral tooth. Left mouthhook with flattened posterior margin; with two distinct acute teeth. Mouthhooks and intermediate sclerite ventrally are strongly sclerotized; intermediate sclerite dorsally and dorsal and ventral cornua very weakly so. Dorsal cornu more sclerotized in anterior third. Indentation index 84.

Female head. ( Figs. 219, 220 ) Brown, with gena and labellum orange, ocellar tubercle black; fronto-orbital plate not visible laterally; 2 ors, 1–2 ori. 1 st fl small, spherical, covered with thick pale pubescence. Gena wider at rear, in widest part 0.3× as wide as maximum height of eye. Vibrissa long, straight, 0.46× as long as maximum height of eye.

Wing. ( Fig. 221 ) Hyaline, with brown veins; costa ending slightly after R 3+4; second cross vein absent; second cross vein absent; calypter pale grey, margin and fringe black. Wing length 2.1 mm.

Female genitalia. ( Figs. 223–226 ) Spermathecae small, 0.08× as high as anterior part of oviscape. Proctiger generally cylindrical, slightly expanding posteriorly. Pair of long setae located on posterior margin between cerci. Cercus oval, 0.23× as long as proctiger; medium setae located subapically, brush of short setae located apically. Spermathecae subequal in size, brown, spherical. Spermathecal duct very weakly sclerotized; with cylindrical pigmented zone near the base of the capsule of spermatheca that is 0.5× as long as height of spermatheca. Ventral receptacle brown, S-shaped. Body of receptacle large, spherical; flattened side with opening, 0.54× as wide as length of receptacle. Basal connecting tube rather wide, slightly curved, 2.5× as wide as tail of receptacle, tail apically with 2 weakly sclerotized narrow blades.

Distribution. Belgium, Bulgaria, the Czech Republic, Denmark, France, Germany, Great Britain, Hungary, Lithuania, the Netherlands, Poland, Romania, Slovakia, Sweden, Kyrgyzstan, Canada, USA ( Papp & Černý 2019). Ukraine (first record).

Comments. Fresh mines ( Fig. 55 ) are greenish due to a wide area of frass located centrally along the full length of the mine and occupying almost its entire width. In 2–3 weeks the mine and frass dry and become whitish ( Fig. 56 ). In Ukraine the earliest dried mines were found in the beginning of June and later fresh mines were collected at the end of June, July and August in the same locality, suggesting that three or four generations developing during the year.

Figures

FIGURES 55–61. Figures 55–58: Phytomyza agromyzina Meigen; 55: mines with larvae in Cornus sanguinea leaf; 56: dried empty mines in Cornus sanguinea leaf; 57: empty puparium viewed from the side; 58: posterior segments of puparium (posterior view). Figures 59–61: Phytomyza astrantiae Hendel; 59: mine in Astrantia major leaf; 60: empty puparium viewed from the side; 61: posterior segments of puparium (posterior view).

FIGURES 219–226. Phytomyza agromyzina Meigen; 219: frontal view of head; 220: head viewed from the side; 221: wing; 222 a, b: cephalopharingeal skeleton; a: total viewed from the side; b: right mouthhook; 223: oviscape, ventral receptacle, proctiger and spermathecae proportionally; 224: spermatheca; 225: proctiger; 226: ventral receptacle.