Phyllozyma guizhouensis Z. W. Xi & F. L. Hui, 2025

Xi, Zhi-Wen, Chai, Chun-Yue, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Phylogeny of Phyllozyma (Spiculogloeaceae, Spiculogloeales) with description of four new species from China, MycoKeys 123, pp. 235-251 : 235-251

Phyllozyma guizhouensis Z. W. Xi & F. L. Hui sp. nov.

Fig. 6

Etymology.

The specific epithet “ guizhouensis ” refers to the geographic origin of the type strain, East Mountain Park, Guiyang City, Guizhou Province.

Typus.

China • Guizhou Prov.: Guiyang City, East Mountain Park , in the phylloplane of Aucuba japonica , 15 Sept 2023, D. Lu, NYNU 239199 ( holotype CICC 33628 T preserved as a metabolically inactive state, culture ex-type PYCC 9994 ) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is pale-yellow, butyrous, smooth, and glossy, with an entire margin. After 3 days in YM broth at 20 ° C, cells are cylindrical, 2.2–3.5 × 7.0–10.4 μm and single, budding is polar. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA, or V 8 agar. Ballistoconidia are falcate or cylindrical, 2.8–3.4 × 7.9–9.6 μm. Glucose fermentation is absent. Glucose, inulin, sucrose, raffinose (delayed), trehalose, D-arabinose (delayed), glycerol, ribitol (delayed and weak), D-mannitol, D-glucitol (delayed), DL-lactate, and succinate are assimilated as sole carbon sources. Melibiose, galactose, lactose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, 5 - keto-D-gluconate, D-ribose, methanol, ethanol, erythritol, galactitol, myo-inositol, citrate, D-gluconate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate, D-glucuronate, and D-glucono- 1, 5 - lactone are not assimilated. Nitrate, nitrite (delayed and weak), and L-lysine are assimilated as sole nitrogen sources. Ethylamine and cadaverine are not assimilated. Maximum growth temperature is 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Hainan Prov.: Wuzhishan City, Wuzhi Mountain , in the phylloplane of Ervatamia divaricata , 12 Aug 2024, Y. F. Lu & F. L. Hui, NYNU 248104 .

GenBank accession numbers.

Holotype CICC 33628 T ( ITS: OR 958770, D 1 / D 2: OR 958769, TEF 1: PV 654549); additional strain NYNU 248104 ( ITS: OR 961462, D 1 / D 2: OR 958779, TEF 1: PV 654550).

Note.

Physiologically, P. guizhouensis sp. nov. differs from its closely related species P. subbrunnea in its ability to assimilate inulin and its inability to assimilate citrate. Furthermore, P. guizhouensis sp. nov. can grow at 30 ° C, while P. subbrunnea cannot (Table 2 ).

Figures

Figure 6. Morphological characteristics of P. guizhouensis sp. nov. NYNU 239199 T. A. The streak culture grown on YM agar after 7 d at 20 ° C; B. Budding cells grown in YM broth for 3 d at 20 ° C; C. Ballistoconidia produced on corn meal agar after 7 d at 20 ° C. Scale bars: 10 μm.