Melanocamarosporioides ugamica D. Pem, R. Jeewon, Gafforov & K. D. Hyde

Pem, Dhandevi, Jeewon, Rajesh, Gafforov, Yusufjon, Urinboev, Islomjon, Alrefaei, Abdulwahed Fahad, Senanayake, Indunil C., Esankulov, Alijon & Hyde, Kevin David, 2025, Introducing Melanocucurbitaria uktampratovii sp. nov. and the sexual morph of Melanocamarosporioides ugamica in Melanommataceae (Dothideomycetes, Pleosporales), MycoKeys 115, pp. 19-42 : 19-42

Melanocamarosporioides ugamica D. Pem, R. Jeewon, Gafforov & K. D. Hyde , in Pem et al. Mycol. Progr. 18 (3): 474 (2019)

Fig. 3

Description.

Saprobic on dead branches of Rosa ecae Aitch. Sexual morph: Ascomata 145–335 μm wide, 70–195 μm high, solitary to gregarious, flattened, semi-immersed to superficial, dark brown to black, globose to ovoid, carbonaceous, papillate. Peridium 15–20 μm, two layered, inner layers composed of hyaline to subhyaline cells of textura prismatica, outer layer composed of irregular, thick-walled, brown cells of textura angularis. Hamathecium comprising numerous, 1.3–2.5 µm (n = 10) wide, filamentous, branched, anastomosing, septate, hyaline, pseudoparaphyses. Asci 90–120 × 14.5–16.7 μm (x ̄ = 104.9 × 15.5 µm, n = 10), 8 - spored, bitunicate, fissitunicate, cylindrical-clavate, pedicellate, apically rounded, with a minute ocular chamber. Ascospores 19.2–25.0 × 7.9–10.0 μm (x ̄ = 21.8 × 8.7 µm, n = 10), uni to biseriate, ellipsoidal, muriform, 5–7 transversely septate, with 5–11 vertical septa, slightly constricted at the septa, dark brown, broadly rounded at the ends, smooth and thick-walled. Asexual morph: Undetermined.

Culture characteristics.

Colonies on MEA, reaching 25–30 mm diam. after 3 weeks at 25 ° C, medium dense, circular, flattened to slightly raised, smooth surface, with edge entire, cottony, mycelium composed of septate, branched hyphae, colony from above whitish, reverse whitish gray to reddish brown in center gradually becoming white towards the edges from the below.

Known distribution

(based on molecular data). Uzbekistan ( Pem et al. 2019 b, this study).

Confirmed hosts

(based on molecular data). Lonicera altmannii ( Caprifoliaceae ) ( Pem et al. 2019 b), Rosa ecae (this study).

Material examined.

Uzbekistan • Tashkent Province, Bostanlik District, Ugam-Chatkal National Nature Park, Charvak Reservoir, Chimyon in Western Tien Shan Mountain , on dead branches of Rosa ecae , 21 July 2019, Y. Gafforov, A. Esankulov, YG-S 2-2 ( TASM 6175 ), living culture MFLUCC 24-0466 .

GenBank numbers.

LSU: PQ 453821, SSU: PQ 433587, ITS: PQ 453019.

Notes.

Our new isolate MFLUCC 24-0466 is morphologically and phylogenetically related to Melanocamarosporioides ugamica ( MFLUCC 17-2314) but collected from a different host. Melanocamarosporioides ugamica was reported from Uzbekistan on dead trunk and branches of Lonicera altmannii ( Caprifoliaceae ) ( Pem et al. 2019 b), while our collection was found on dead branches of Rosa ecae . Melanocamarosporioides ugamica ( TASM 6133) is characterized by an asexual morph, namely black, globose, superficial conidiomata and large conidia, which are multiseptate and distinctively dark brown, while our collection ( TASM 6175) is in its sexual state, characterized by globose to ovoid, dark brown to black ascomata, cylindrical asci, and dark brown, ellipsoidal, muriform ascospores. Multi-gene phylogeny ( LSU, SSU, ITS, and TEF- 1) shows that our collection groups with Melanocamarosporioides ugamica ( MFLUCC 17-2314) in a 99 % ML and 1.00 BYPP supported clade. With regard to DNA sequence data comparison, there is a difference of 0.69 % (6 out of 865), 0.84 % (9 out of 1067), and 0 % (0 out of 470) in nucleotide variations within the LSU, SSU, and ITS genes, respectively. Hence, we introduce our collection as a new host record of Melanocamarosporioides from Rosa ecae . This is also the first sexual morph report in Melanocamarosporioides . The phylogenetic placement of our strain TASM 6175 is shown in Fig. 1 .

Figures

Figure 1. Phylogram generated from maximum likelihood analysis based on combined LSU, SSU, ITS and TEF- 1 sequence data for Melanommataceae. The tree is rooted with Cyclothyriella rubronotata (CBS 121892, CBS 141486). The new isolates are in red and ex-type strains and are shown in boldface. Bootstrap support values for maximum likelihood (ML) equal to or greater than 70 % and Bayesian posterior probabilities (BYPP) equal to or greater than 0.90 are given above the nodes, respectively.

Figure 3. Melanocamarosporioides ugamica (TASM 6175, new host record and sexual morph) a, b appearance of ascomata on the host surface c pseudoparaphyses d – g asci h, i ascospores j germinated ascospore k, l culture characteristics on MEA (k above view, l reverse view). Scale bars: 500 μm (a, b); 5 μm (c); 20 μm (d – g, j); 10 μm (h, i).