Camporesiomyces bhatii M. Y. Han & Tibpromma, 2025

Han, Mei-Yan, Karunarathna, Samantha C., Lu, Li, Zheng, De-Ge, Suwannarach, Nakarin, Elgorban, Abdallah M., Dai, Dong-Qin, Zhang, Li-Juan, Zhao, Wan-Tong, Chukeatirote, Ekachai & Tibpromma, Saowaluck, 2025, Three new species of Camporesiomyces (Tubeufiaceae, Tubeufiales, Dothideomycetes) associated with coffee in Yunnan, China, MycoKeys 117, pp. 315-336 : 315-336

Camporesiomyces bhatii M. Y. Han & Tibpromma sp. nov.

Fig. 2

Etymology.

The species epithet “ bhatii ” honors Prof. Jayarama Darbhe Bhat for his immense contributions to mycology.

Holotype.

GMB-W 1176

Description.

Saprobic on dead branches of Coffea liberica . Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies on natural substrate superficial, solitary or in clusters, hairy, yellow at apex, velvety. Mycelium exposed on the surface of substrate, except on the roots. Conidiophores 23–87.4 × 2.4–5.7 μm (x ̄ = 63 × 3.7 μm, n = 30) macronematous, mononematous, either solitary or forming small clusters, smooth or occasionally verruculose, cylindrical, brown, slightly flexuous, simple, unbranched, 2–8 - septate, sometimes slightly constricted at the septa. Conidiogenous cells 8.8–21.7 × 3–4.7 μm (x ̄ = 17 × 3.5 μm, n = 20), polyblastic, terminal, cylindrical, slightly tapering, conspicuously denticulate on conidial secession, pale brown. Conidia 16–30 × 3.3–6.3 μm (x ̄ = 24 × 6.5 μm, n = 30), solitary, acrogenous, cylindrical, obclavate or fusiform, 3–8 - septate, subhyaline to pale brown.

Culture characteristics.

Conidia germinate on PDA within 24 h at 28 ° C, colony on PDA reaching 3 cm diam. after two weeks, circular or irregular, umbonate, with the entire margin dark brown to black. The reverse side displays predominantly black colonies with brown peripheral edges.

Material examined.

China • Yunnan Province, Xishuangbanna Autonomous Prefecture (21°41'N, 101°25'E 570 m), on dead branches of Coffea liberica , 27 August 2024, M. Y. Han & Tibpromma, (DL 4 = GMB-W 1176 , holotype), ex-type GMBCC 1120 , other living culture GMBCC 1125 .

GenBank number.

GMBCC 1120 = ITS: PQ 763360, LSU: PQ 842543, tef 1 - α: PV 388888, rpb 2: PV 388894 and GMBCC 1125 = ITS: PQ 763361, LSU: PQ 842544, tef 1 - α: PV 388889, rpb 2: PV 388895.

Notes.

In the phylogenetic tree, our collection Camporesiomyces bhatii [ GMBCC 1120 (ex-type) and GMBCC 1125 ] formed a well-separated lineage, sister to C. vaccinii [ CBS 216.90 , ex-type] with 96 % ML / 0.99 PP support (Fig. 1 ). ITS and LSU gene sequences blast results showed that our strain showed 94.14 % and 99.23 % similarities to C. vaccinii ( MH 862204 ) and ( MH 873889 ), respectively ( Carris 1989). In the morphological comparison between C. bhatii and C. vaccinii , significant differences are observed in both conidial and conidiophore dimensions, as well as conidial shape ( Carris 1989). The conidia of C. bhatii are notably longer than those of C. vaccinii (16–30 × 3.3–6.3 μm) compared to (8.0–13.0 × 2.0–4.0 μm). Conversely, the conidiophores of C. bhatii are shorter than those of C. vaccinii (23–87.4 × 2.4–5.7 μm) compared to (64–145 × 4.2–5.0 μm) ( Carris 1989). Additionally, the conidia of C. bhatii exhibit complex shapes, such as obclavate or fusiform, and are subhyaline to pale brown. In contrast, the conidia of C. vaccinii are spirally coiled and hyaline or pale brown ( Carris 1989). These distinct morphological characteristics provide a clear basis for differentiating the two species. Therefore, based on molecular data and morphological comparison, C. bhatii was identified as a new species.

Figures

Figure 1. The ML analysis and Bayesian inference (BI) analyses yielded nearly identical tree topologies, with bootstrap support values for ML equal to or greater than 50 %, and BI analysis values equal to or greater than 0.90 PP are provided at each node. Newly generated sequences are in red, while the ex-type strains are in bold.

Figure 2. Camporesiomyces bhatii (GMB-W 1176, holotype) a, b colonies on the natural substrate c, d conidiophore with conidia and conidiogenous cells e, f conidiogenous cells g – m conidia n a germinated conidium o, p Culture on PDA from above and reverse. Scale bars: 50 μm (c); 20 μm (d); 10 μm (e, g – n); 5 μm (f).