Apiospora intestini (Kajale, Sonawane and Roh. Sharma) Pintos and P. Alvarado

Absalan, Sahar, Armand, Alireza, Jayawardena, Ruvishika S., Suwannarach, Nakarin, Monkai, Jutamart, Jungkhun, Nootjarin, Lumyong, Saisamorn & Hyde, Kevin D., 2025, Endophytic xylariaceous fungi from rice in northern Thailand: discovery of novel species and new host records, MycoKeys 120, pp. 1-34 : 1-34

Apiospora intestini (Kajale, Sonawane and Roh. Sharma) Pintos and P. Alvarado , Fungal Syst. Evol. 7: 206 (2021)

Fig. 1

Description.

Endophytic from healthy leaf of Oryza sativa . Sexual morph: not observed. Asexual morph: Hyphae 4–5.5 μm wide, septate, thick-walled, hyaline to brown. Conidiophores, conidiogenous cells, and conidia not observed.

Culture characteristics.

Colonies on PDA reaching 86–90 mm in diameter after 7 days at 28 ° C, white, medium dense with some immersed dark brown mycelia, cottony; reverse white.

Material examined.

Thailand • Chiang Rai Province, Phan District, Mueang Phan Subdistrict , from healthy tissue part of rice leaf, 5 January 2022, Sahar Absalan (NS 50-1 a = MFLU 25-0029 ) (living culture MFLUCC 24-0510 ) .

GenBank numbers.

MFLUCC 24-0510 : ITS = PV 235258, tef 1 - α = N / A, tub 2 = PV 275687.

Notes.

Apiospora intestini was introduced by Crous et al. (2015) as Arthrinium gutiae isolated from the gut of a grasshopper. It was also isolated as a saprobe from bamboo in Chiang Rai Province, Thailand ( Tian et al. 2021 a). Phylogenetic analysis of a concatenated ITS, tef 1 - α, and tub sequence dataset indicated that our strain ( MFLUCC 24-0510 ) has a close affinity with A. intestini with 98 % ML and 0.98 PP support (Fig. 2 ). However, we were not able to compare the morphological characteristics because our isolate remained sterile in culture. Therefore, we report this species as a new host record from rice.

Figures

Figure 1. Apiospora intestini (MFLUCC 24-0510, new host record). a. Front and reverse of the colony on PDA; b – e. Hyphae. Scale bars: 20 μm (c – e).

Figure 2. Phylogram of ML analysis based on combined ITS, tef 1 - α, and tub 2 sequence data. ML bootstrap support values equal to or higher than 60 % and Bayesian probability values (PP) equal to or above 0.80 are given at the nodes (ML / PP). The tree is rooted to Nigrospora gorlenkoana (CBS 480.73) and N. camelliae-sinensis (CGMCC 3.18125). The isolate from the current study is highlighted in red, and type strains are indicated in bold black.