Pestalotiopsis disseminata (Thüm.) Steyaert

Wang, Quan Chao, Zhan, Zhao Jie, Sattar, Adil, Wang, Hao Nan, Zhou, Li Feng, Eckhardt, Lori, Li, Guo Qing, Liu, Fei Fei, Xu, Hua Chao & Zhou, Xu Dong, 2025, Pestalotiopsis (Amphisphaeriales, Sporocadaceae) species including six new taxa inhabiting pines from different climate zones in China, IMA Fungus 16, pp. e 151614-e 151614 : e151614-

Pestalotiopsis disseminata (Thüm.) Steyaert , Bull. Jard. bot. Etat Brux. 19: 319. (1949).

Fig. 5

Description.

Sexual state not seen. Conidiomata in culture sporodochial, saucer-shaped, scattered or gregarious, superficial to immersed, shining, releasing black conidial masses on the surface. Conidiophores branched, subcylindrical, hyaline to light brown, indistinct, often reduced to conidiogenous cells. Conidiogenous cells cylindrical or ampulliform, hyaline, smooth-walled, solitary to aggregated, (5.5 –) 7–10 (– 11.5) × 2–3 μm (x ± SD = 8.4 ± 1.6 × 2.4 ± 0.4 μm). Conidia fusoid, ellipsoid, smooth, slightly constricted at the septa, 4 - septate, (8 –) 17.5–25.5 (– 26) × (4 –) 6–7.5 (– 8) μm (x ± SD = 21.6 ± 4.1 × 6.7 ± 0.8 μm); apical cell with 2–3 tubular appendages (mostly three); apical appendages arising from an apical crest, unbranched, filiform, bent, (3.5 –) 10.5–16.5 (– 22) μm (x ± SD = 13.6 ± 3.1 μm) long; basal cell with one appendage; basal appendage tubular, centric appendage tubular, unbranched, occasionally swollen at the tip, (2 –) 2.5–4 (– 4.5) μm (x ± SD = 3.4 ± 0.7 μm) long.

Materials examined.

CHINA • Zhejiang Province, Ningbo City, Xiangshan County, Fengmenkou Forest Park , 29°5'44"N, 121°57'19"E, on diseased needle of Pinus elliottii , 19 April 2023, Quanchao Wang & Guiyong Cao (culture ZXD 34) ; • Shandong Province, Qingdao City, Jimo District, Tianheng Island Resort , 36°25'2"N, 120°51'37"E, on diseased needle of Pinus thunbergii , 9 August 2023, Quanchao Wang, Guoqing Li, Feifei Liu & Runlei Chang (cultures ZXD 526, ZXD 529, ZXD 530, ZXD 532, ZXD 546, ZXD 552, ZXD 553, ZXD 577, ZXD 578, ZXD 579, ZXD 580, ZXD 582, ZXD 585, ZXD 586, ZXD 587, ZXD 588) .

Notes.

Pestalotiopsis disseminata was first reported from Eucalyptus botryoides in Portugal ( Von Thümen 1881) and subsequently reported on a wide range of hosts and locations, including the genus Pinus ( Hu et al. 2007; Liu et al. 2019; Silva et al. 2020). In this study, 17 strains were isolated from P. elliottii and P. thunbergii and clustered together with Pes. disseminata in the multi-locus phylogenetic tree (Fig. 3 ). Compared to the description of the isolate CBS 118552, ZXD 579 exhibits smaller conidiogenous cells (5.5–11.5 × 2–3 μm vs. 7–24.5 × 2–5 μm), while other morphological characteristics are similar. This is the first report of Pes. disseminata on the host of P. elliottii and P. thunbergii .

Figures

Figure 3. Phylogenetic trees based on Maximum Likelihood (ML) analyses from the multi-gene alignment (ITS, tef- 1 α and tub 2) for Pestalotiopsis. The ML bootstrap values (left; values of ≥ 60 % are shown, < 60 % are marked with * and absence is marked with -) and Bayesian posterior probabilities (right; values of ≥ 0.9 are shown, < 0.9 are marked with * and absence is marked with -) are indicated above the branches. Isolates representing ex-type material are marked with “ T ”. Neopestalotiopsis magna (MFLUCC 12-0652) was used as the outgroup taxon.

Figure 5. Morphology of Pestalotiopsis disseminata (ZXD 579). A Colony on MEA after 10 days at 25 ° C; B colony on PDA after 10 days at 25 ° C; C conidiomata formed on MEA; D, E conidiogenous cells giving rise to conidia; F – K conidia. Scale bars: 500 μm (C); 20 μm (D – G).