Pestalotiopsis guangxiensis Ning Jiang

Wang, Quan Chao, Zhan, Zhao Jie, Sattar, Adil, Wang, Hao Nan, Zhou, Li Feng, Eckhardt, Lori, Li, Guo Qing, Liu, Fei Fei, Xu, Hua Chao & Zhou, Xu Dong, 2025, Pestalotiopsis (Amphisphaeriales, Sporocadaceae) species including six new taxa inhabiting pines from different climate zones in China, IMA Fungus 16, pp. e 151614-e 151614 : e151614-

Pestalotiopsis guangxiensis Ning Jiang , Microbiol. Spectr. 6: 14 (2022).

Fig. 6

Description.

Sexual state not seen. Conidiomata in culture sporodochial, saucer-shaped, scattered or gregarious, superficial to immersed, shining, releasing black conidial masses on the surface. Conidiophores branched, subcylindrical, hyaline to light brown, indistinct, often reduced to conidiogenous cells. Conidiogenous cells cylindrical or ampulliform, hyaline, smooth-walled, solitary to aggregated, (7.5 –) 9–13 (– 14.5) × (1.5 –) 3–4 (– 5) μm (x ± SD = 11 ± 1.9 × 3.5 ± 0.7 μm). Conidia fusoid, ellipsoid, smooth, slightly constricted at the septa, 4 - septate, (16 –) 17.5–20.5 (– 22) × (4 –) 6.5–9 (– 9.5) μm (x ± SD = 19 ± 1.6 × 7.6 ± 1.2 μm); apical cell conic with 2–4 tubular appendages (mostly four); apical appendages arising from an apical crest, unbranched, filiform, bent, (7 –) 11–16 (– 18.5) μm (x ± SD = 13.4 ± 3.3 μm); basal cell obconic with one appendage, basal appendage tubular, centric appendage tubular, unbranched, occasionally swollen at the tip, (2.5 –) 3–4 (– 4.5) μm (x ± SD = 3.4 ± 0.6 μm) long.

Materials examined.

CHINA • Zhejiang Province, Wenzhou City, Cangnan County, Dayu Town , 27°22'37"N, 120°36'48"E, on diseased needle of Pinus massoniana , 6 May 2023, Quanchao Wang, Guiyong Cao (cultures ZXD 63, ZXD 67, ZXD 71, ZXD 72, ZXD 89, ZXD 103) ; • Guangdong Province, Shanwei City, Zhelang Street , 22°47'29"N, 115°32'18"E, on diseased leaves of Pinus massoniana , 27 August 2023, Quanchao Wang, Guoqing Li, Feifei Liu & Runlei Chang (culture ZXD 952) ; • Guangdong Province, Jiangmen City, Taishan County, Chixi Town , 21°53'10"N, 112°57'0"E, on diseased needle of Pinus massoniana , 28 August 2023, Quanchao Wang, Guoqing Li, Feifei Liu & Yuhua Liang (cultures ZXD 960, ZXD 963, ZXD 971, ZXD 972) .

Notes.

Pestalotiopsis guangxiensis was first reported from Quercus griffithii in China ( Jiang et al. 2022 c). In this study, 11 strains were isolated from P. massoniana and clustered together with Pes. guangxiensis in the multi-locus phylogenetic tree (Fig. 3 ). Compared with the description of ex-type isolate CFCC 54308 ( Jiang et al. 2022 c), isolate ZXD 960 has shorter apical appendages (7–18.5 μm vs. 14–19 μm), while other morphological characteristics are similar. This is the first report of Pes. guangxiensis on the host of P. massoniana .

Figures

Figure 3. Phylogenetic trees based on Maximum Likelihood (ML) analyses from the multi-gene alignment (ITS, tef- 1 α and tub 2) for Pestalotiopsis. The ML bootstrap values (left; values of ≥ 60 % are shown, < 60 % are marked with * and absence is marked with -) and Bayesian posterior probabilities (right; values of ≥ 0.9 are shown, < 0.9 are marked with * and absence is marked with -) are indicated above the branches. Isolates representing ex-type material are marked with “ T ”. Neopestalotiopsis magna (MFLUCC 12-0652) was used as the outgroup taxon.

Figure 6. Morphology of Pestalotiopsis guangxiensis (ZXD 89). A Colony on MEA after 10 days at 25 ° C; B colony on PDA after 10 days at 25 ° C; C conidiomata formed on MEA; D, E conidiogenous cells giving rise to conidia; F – K conidia. Scale bars: 500 μm (C); 20 μm (D – G).