Filobasidium qingyuanense C. Y. Cai & F. L. Hui, 2025

Chai, Chun-Yue, Xi, Zhi-Wen, Niu, Qiu-Hong & Hui, Feng-Li, 2025, Phylogeny and phenotype of Filobasidium revealing three new species (Filobasidiaceae, Filobasidiales) from China, MycoKeys 114, pp. 49-65 : 49-65

Filobasidium qingyuanense C. Y. Cai & F. L. Hui sp. nov.

Fig. 3 C

Etymology.

The specific epithet qingyuanense refers to the geographic origin of the type strain, Qingyuan Mountain, Quanzhou City, Guangdong Province.

Typus.

China • Guangdong Province, Quanzhou City, Qingyuan Mountain , in the phylloplane of Lespedeza formosa , March 2022, W. T. Hu and S. B. Chu, NYNU 223211 (holotype GDMCC 2.309 T preserved as a metabolically inactive state in the Guangdong Microbial Culture Collection Center, culture ex-type PYCC 9927 deposited in the Portuguese Yeast Culture Collection) .

Description.

On YM agar, after 7 days at 20 ° C, the streak culture is gray-cream, mucoid, smooth, and glossy. The margin is entire. On YM agar, after 7 days at 20 ° C, cells are globosal and ellipsoidal, 6.7–10.2 × 7.6–10.4 μm and single, budding is polar. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on PDA, CM agar, and YCBS agar for two months. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, glycerol (weak), ribitol, D-mannitol, D-glucitol, myo-inositol, succinate, citrate, D-gluconate, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone are assimilated as sole carbon sources. L-Sorbose, D-ribose, methanol, ethanol, erythritol, galactitol, DL-lactate, D-glucosamine, and N-acetyl-D-glucosamine are not assimilated. Nitrate, nitrite, ethylamine, and L-lysine are assimilated as sole nitrogen sources. Cadaverine is not assimilated. Maximum growth temperature is 30 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Guizhou Province, Qianxinan Buyei and Miao Autonomous Prefecture, Pingtang County, in the phylloplane of Mussaenda pubescens , February 2023, D. Lu, NYNU 23239 .

GenBank accession numbers.

Holotype GDMCC 2.309 T (ITS: OP 278683 , D 1 / D 2: OP 278680 ); additional strain NYNU 23239 (ITS: PP 114093, D 1 / D 2: PP 114095).

Note.

Filobasidium qingyuanense sp. nov. can be physiologically distinguished from its closest known species, F. dingjieense , by its ability to assimilate inulin, raffinose, melibiose, lactose, salicin, L-rhamnose, ribitol, D-mannitol, and D-glucitol, as well as an inability to assimilate ethanol. Additionally, F. qingyuanense nov. can grow at 25 ° C, while F. dingjieense cannot (Table 3 ).

Figures

Figure 3. Vegetative cells of F. pseudomali sp. nov. NYNU 228108 T (A), F. castaneae sp. nov. NYNU 2111105 T (B), and F. qingyuanense sp. nov. NYNU 223211 T (C) following growth in YM broth for 7 days at 20 ° C. Scale bars: 10 μm.