Dermoloma pruinosipes Corriol & Jančovič., 2025

Adamčíková, Katarína, Kiran, Munazza, Caboň, Miroslav, Matheny, Brandon P., Sánchez-García, Marisol, Arnolds, Eef, Caboňová, Michaela, Corriol, Gilles, Dima, Bálint, Friebes, Gernot, Griffith, Gareth W., Grootmyers, Django, Harries, David, Karich, Alexander, Mešić, Armin, Mihaljevič, Martin, Moreau, Pierre-Arthur, Pošta, Ana, Shapkin, Vasilii, Tkalčec, Zdenko, Vizzini, Alfredo, Vondrovicová, Lenka, Adamčík, Slavomir & Jančovičová, Soňa, 2025, A phylogenetic and morphological study of the genus Dermoloma (Agaricales, Tricholomataceae) in Europe and North America exposes inefficiency of opportunistic species descriptions, IMA Fungus 16, pp. e 157337-e 157337 : e157337-

Dermoloma pruinosipes Corriol & Jančovič. sp. nov.

Figs 39 e, f , 43 a , 44

Etymology.

In reference to the distinctly pruinose stipe.

Holotype.

Slovakia • Kremnické vrchy Mts., pasture 0.5 km W of Tajov , elev. 600 m, coord. 48°44'54"N, 19°03'31"E, terrestrial, 25 Oct 2020, S. Jančovičová ( SAV F-20834 ).

Diagnosis.

European species; basidiomata small, mycenoid or collybioid; pilei 9–20 mm, when wet translucently striate near margin and brown to dark brown, when dry light brown; stipes 1–3 mm wide, when young entirely pruinose; lamellae brownish ochraceous, brownish gray or grayish brown; spores amyloid, 6–7.1 × 3.9–4.4 μm; marginal cells lageniform, with long, narrow, flexuous, nodulose or diverticulate apical projections; caulocystidia, 5–18 μm wide, very variable in shape and size and often flexuous.

Pileus (7 –) 9–20 (– 30) mm; convex, later plano-convex, sometimes indistinctly umbonate or broadly conical; margin translucently striate to half of the radius when wet; surface smooth near margin, usually radially rugulose or rough near center, hygrophanous and starting to discolor from center; color when young dark brown (6 F 4, 6 F 5), when mature near margin dark brown (6 F 4, 6 F 5, 6 F 6), sometimes grayish brown (6 D 3), when dry light brown (5 D 4), brownish ochraceous (5 C 3, 5 C 4, 6 C 4), grayish brown (5 D 3), grayish ochraceous (5 B 4), near center dark brown (6 F 4, 6 F 6, 7 F 3), rarely brown (6 E 4), when dry brown (6 E 6), light brown (5 D 5, 5 D 4), grayish brown (5 D 3), brownish ochraceous (6 C 4). Stipe (22 –) 25–40 (– 50) × 1–3 (– 4) mm; cylindrical, narrowed towards the base, flexuous; surface finely longitudinally striate, first entirely pruinose, then pruinose near lamellae, towards the base glabrous and shiny; color near lamellae brownish ochraceous (5 C 3, 6 C 3, 6 C 4), brownish gray (5 D 2), grayish brown (5 D 3, 6 D 3,6E 3) to brown (6 E 4), near the base light brown (6 D 4), grayish brown (6 E 3), brown (6 E 4), dark brown (6 F 3, 6 F 4, 6 F 5, 7 F 4) Lamellae L = (13 –) 17–28, l = (0 –) 1–3 (– 5); 2.5–4 mm wide; adnexed or adnate-emarginate; color ochraceous-gray (5 B 2), brownish ochraceous (5 C 4, 6 C 4), brownish gray (6 C 2, 6 D 2), grayish brown (6 D 3); edges entire. Context elastic to fragile; odor indistinct.

Spores (5.2 –) 6–6.5 – 7.1 (– 8) × (3.6 –) 3.9–4.1 – 4.4 (– 5) μm; narrowly ellipsoid to oblong, Q = (1.32 –) 1.45–1.59 – 1.72 (– 2.00); walls amyloid; hilar appendage 0.5–1 μm long. Basidia (20 –) 26–29.2 – 32 (– 34) × (5.5 –) 6.5–7 – 7.5 (– 9) μm; clavate; with 4 sterigmata. Basidioles first cylindrical, then clavate, ca. 3–6 μm wide. Marginal cells (18 –) 21.5–28.5 – 35.5 (– 53) × (3.5 –) 4–5 – 6 (– 8.5) μm; lageniform, with long, narrow, flexuous, nodulose or diverticulate apical projections. Pileipellis 48–60 μm deep; suprapellis 28–35 μm deep, usually of one or three layers of inflated, densely arranged cells; subpellis well-differentiated, 20–30 μm deep, of densely packed, puzzled, 2.5–10 μm wide hyphae, gradually passing to horizontally oriented hyphae in trama; hyphal terminations with brownish yellow parietal pigments, in subpellis near center also with incrusted brownish yellow pigments, walls thickened up to 0.5 μm, in subpellis up to 1.5 μm. Terminal cells near pileus margin (18 –) 28.5–37.7 – 47 (– 67) × (10 –) 13.5–18.8 – 24 (– 35) μm; usually obpyriform, sphaeropedunculate or clavate; subterminal cells usually unbranched, fusiform or clavate and ventricose, rarely cylindrical, often with lateral swellings or branches. Terminal cells near pileus center (20 –) 27.5–38.3 – 49 (– 72) × (7 –) 13.5–19.1 – 24.5 (– 42) μm; similar to cells near margin; subterminal cells usually narrower, narrowly fusiform. Caulocystidia (15 –) 18.5–26.8 – 35 (– 48) × 5–11.5 – 18 (– 42) μm; very variable in shape and size, some small and clavate, others large and obpyriform, sphaeropedunculate, often flexuous, sometime with projections or lobate, repent and in small or larger clusters, often intermingled, subterminal cells often with lateral projections or branches; usually with slightly thickened walls up to 0.5 μm, but near septa and on subterminal cells up to 1 μm, with brownish yellow parietal pigments. Clamp connections present.

Distribution and ecology.

Known from Austria, Croatia, Germany, The Netherlands, Norway and Slovakia; in semi-natural grasslands on calcareous soil.

Additional material studied.

Austria • Burgenland, Oberwart, Rechnitz, Galgenberg , elev. 344 m, coord. 47°17'52"N, 16°25'09"E, semi-dry grassland, soil among moss and grass, 17 Nov 2019, G. Friebes GF 20190130 ( SAV F-23423 ) . Croatia • Kamenjak peninsula, 3.5 km S-SE of Premantura, near Pula , coord. 44°46'17"N, 13°55'06"E, grassland with Rosmarinus sp. , 20 Nov 2005, M. Čerkez ( CNF 1/3789 ) . France • Hautes-Pyrénées, Arrens-Marsous, Caytivère , coord. 42°58'51"N, 00°14'53"E, acidophilic mountain grazed grassland (Nardion), 4 Oct 2016, C. Hannoire CH 16100418 ( BBF, as D. phaeopodium ) ; • Hautes-Pyrénées, Campan, vallon du Garet , coord. 42°55'06"N, 00°12'35"E, neutrophilous mountain grassland, 8 Oct 2016, G. Corriol GC 16100816 ( BBF, as D. pseudocuneifolium var. obscurum ) ; • Puy-de-Dôme, Orcines, Puy de la Charité , coord. 45°46'17"N, 03°01'42"E, lawn on volcanic soil (Koelerio-Phleion), 16 Oct 1999, G. Corriol GC 99101603 ( BBF, as D. phaeopodium ) . Germany • Altranft, Hütelandschaft , coord. 52°45'19"N, 14°03'42"E, on loamy alkaline soil in extensively grazed (sheep) semi-natural grassland, 6 Nov 2022, A. Karich, R. Ullrich and R. Jarling IHI- 22 Der 05 ( GLM-F 137757 ) ; • Baden-Württemberg, Justingen, Schachenheide , coord. 48°24'35"N, 09°40'25"E, terrestrial in semi-natural grassland, 2 Oct 2021, S. Adamčík ( SAV F-20894 ) ; • ibid., 3 Oct 2021, S. Adamčík ( SAV F-20914 ) . The Netherlands • Beilen, nature area Schepping , in poor grassland on calcareous loam, 1 Oct 2004, E. Arnolds Arnolds 04-110 ( L, as D. rancidum n. prov.) . Norway • Akershus, Jevnaker, Rustad , semi-natural, calcareous grassland, 16 Aug 2023, T. E. Brandrud TEB 332-23 ( O) . Slovakia • Kremnické vrchy Mts., pasture 0.5 km W of Tajov , elev. 600 m, coord. 48°44'54"N, 19°03'31"E, terrestrial, 24 Oct 2020, S. Adamčík ( SAV F-20815 ) ; • ibid., 25 Oct 2020, M. Caboň ( SAV F-20845 ) ; • Poloniny Mts., 4 km N of Stakčín, pastures above the water reservoir Stariná , elev. 380–420 m, coord. 49°02'431"N, 22°14'56"E, terrestrial, 25 Sep 2017, S. Adamčík ( SAV F-20230 ) .

Notes.

Dermoloma pruinosipes is a member of D. subgenus Amylospora , section Atrobrunnea . It belongs to an inclusive clade of species with mainly collyboid basidiomata, all of which are morphologically similar. For more comments and recommendations, see the notes under the previous species. Compared to other members of the clade it is relatively distinct, as the stipes are typically distinctly pruinose along their entire length and the marginal cells have long attenuated apical projections. The species cluster of D. pruinosipes includes 14 samples from seven countries, but it was represented only by a single sample in the previous phylogeny by Sánchez-García et al. (2021) and labelled as “ Dermoloma sp. 8 ”. It is represented here by three distinct sequence variants in the ITS tree (Suppl. material 8), but the multilocus phylogeny (Fig. 2 ) did not support to distinguish them as three separate taxa and we did not find distinct morphological differences to distinguish them.

Figures

Figure 2. Phylogram generated by ML analysis based on combined sequence data of ITS, LSU, rpb 1, rpb 2, mcm 7 and tef 1 a showing phylogenetic relationships within genus Dermoloma. ML bootstrap support values greater or equal to 50 % and Bayesian posterior probabilities greater or equal to 0.90 are indicated above or below the nodes. Sequences originating from type collections are labelled in red and bold.

Figure 39. Basidiomata of Dermoloma in field appearance. a Dermoloma obscurum (SAV F- 20553), photo S. Jančovičová; b Dermoloma parvisporum (SAV F- 20229, holotype), photo S. Jančovičová; c Dermoloma phaeopodium (SAV F- 20546), S. Jančovičová; d Dermoloma phaeopodium (SAV F- 22208), photo S. Jančovičová; e Dermoloma pruinosipes (SAV F- 20834, holotype), photo S. Jančovičová; f Dermoloma pruinosipes (SAV F- 20504), photo S. Jančovičová. Scale bar: 10 mm.

Figure 43. Basidiomata of Dermoloma in field appearance. a Dermoloma pruinosipes (GLM-F 137757), photo A. Karich; b Dermoloma aff. pruinosipes, (SAV F- 23429), photo G. Friebes; c Dermoloma pseudojosserandii [BBF (CH 17100504)], holotype, photo C. Hannoire; d Dermoloma pseudojosserandii [BBF (GC 17092306)], photo G. Corriol; e Dermoloma rostratum (SAV F- 20811, holotype), photo S. Jančovičová; f Dermoloma rostratum (SAV F- 20817), photo S. Jančovičová. Scale bar: 10 mm.

Figure 44. Dermoloma pruinosipes (SAV F- 20230), microscopic elements. a Spores; b caulocystidia; c basidia and basidioles; d marginal cells; e pileipellis elements near the pileus margin; f pileipellis elements near the pileus center. Scale bar: 5 µm for spores and 10 µm for other elements.