Diaporthe tulliensis R. G. Shivas, Vawdrey & Y. P. Tan

Phukhamsakda, Chayanard, Hyde, Kevin D., Samarakoon, Milan C., Louangphan, Johnny, Navasit, Kedsara, Al-Otibi, Fatimah & Bhunjun, Chitrabhanu S., 2025, Unveiling Sordariomycetes taxa associated with woody litter in Northern Thailand, MycoKeys 115, pp. 155-185 : 155-185

Diaporthe tulliensis R. G. Shivas, Vawdrey & Y. P. Tan , Persoonia 35: 301 (2015)

Fig. 6

Description.

Saprobic on decaying Bambusa . Sexual morph: Undetermined. Asexual morph: Conidiomata 91–148 × 311–974 μm (x ̄ = 120 × 583 μm, n = 20), pycnidial, scattered or aggregated, embedded in host surface, slightly erumpent through host surface, 1–3 - locular conidioma, nearly flat, elongated, discoid, or variable in shape, black, consisting of hyaline, thin-walled cells of textura angularis, outer layer thick walled. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 4–16 × 1.2–2.6 μm (x ̄ = 7.5 × 1.8 μm, n = 40), cylindrical, unbranched, aseptate, smooth, straight or slightly curved, tapering towards the apex, wider at base, hyaline. Alpha conidia 4.1–7.8 × 1.7–3.1 μm (x ̄ = 5.8 × 2.5 μm, n = 40), apex bluntly rounded, 1–2 - guttulate, mostly bi-guttulate, oval or oblong to ellipsoid, hyaline, smooth, base obtuse to subtruncate. Beta and Gamma conidia not observed.

Culture characteristics.

Colonies on PDA, reaching 40 mm diam., after 2 weeks at 25 ° C, initially white, turning olivaceous grey after 7–10 days, darker at the centre and marginal area, lacking aerial mycelium; reverse, olivaceous grey bordered by dark margins.

Material examined.

Thailand, Chiang Mai Province, Mae Taeng District , on dead terrestrial stem of Bambusa ( Poaceae ), 19 November 2022, J. Louangphan, MJ 11 ( MFLU 23–0475 ); living culture, MFLUCC 24–0524 = MFLUCC 23–0301 .

Hosts.

Actinidia spp. ( Actinidiaceae ), Alangium kurzii ( Cornaceae ), Bambusa sp. ( Poaceae ), Bougainvillea glabra ( Nyctaginaceae ), Celtis formosana ( Ulmaceae ), Morinda officinalis ( Rutaceae ), Tectona grandis ( Lamiaceae ), Theobroma cacao ( Malvaceae ), Soil, Vitis vinifera ( Vitaceae ) ( Chang et al. 2005; Crous et al. 2015; Bai et al. 2017; Doilom et al. 2017; Yang et al. 2018; Manawasinghe et al. 2019; Tennakoon et al. 2021; Luo et al. 2022; this study).

Distribution.

Australia, China, Korea, Thailand ( Chang et al. 2005; Crous et al. 2015; Bai et al. 2017; Doilom et al. 2017; Yang et al. 2018; Manawasinghe et al. 2019; Tennakoon et al. 2021; Luo et al. 2022; this study).

Notes.

In the phylogenetic analysis, our isolates ( MFLUCC 23–0301 and MFLUCC 24–0524 ) clustered with D. tulliensis isolates ( MFLUCC 14–1139 , JZB 320128, and BRIP 62248 a) with 100 % ML / 1.00 BPP support (Fig. 1 ). Our isolate has a similar morphology to D. tulliensis isolates but differs from D. tulliensis in the size of conidiomata (up to 500 µm (= D. celtidis and D. tulliensis ) vs. up to 510 µm (= D. hubeiensis ) vs. 135–330 μm (= D. alangii ) vs. 50–380 μm (= D. morindae ) vs. 725–820 μm diam. (= D. tectonae )) ( Crous et al. 2015; Doilom et al. 2017; Yang et al. 2018; Manawasinghe et al. 2019; Luo et al. 2022). Our isolate also differs due to the absence of beta conidia, which has been reported in some D. tulliensis isolates ( Chang et al. 2005; Crous et al. 2015; Doilom et al. 2017; Manawasinghe et al. 2019). Therefore, we report our isolate as a new host record of D. tulliensis .

Figures

Figure 1. Phylogram of the Diaporthe sojae species complex generated from a maximum likelihood analysis based on the combined ITS, tef 1, tub 2, cal, and his 3 sequence data. Diaporthella corylina (CBS 121124) was used as the outgroup. Bootstrap support values ≥ 60 % ML / ≥ 0.90 BPP are given at the nodes. The newly generated taxa are indicated in red. The holotype / ex-type strains are denoted with T.

Figure 6. Diaporthe tulliensis (MFLU 23–0475) a, b conidiomata on host c longitudinal section through conidioma d, e transverse section of conidioma f, g conidiogenous cells giving rise to conidia h, i alpha conidia j, k germinated conidia l front and reverse view of the colony on PDA. Scale bars: 200 µm (b – d); 20 µm (e); 10 µm (f – k).